BSND (Myc-DDK-tagged)-Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
BSND (Myc-DDK-tagged)-Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
BSND (tGFP-tagged) - Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
BSND - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
Lenti ORF clone of Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND), mGFP tagged
Type | Human Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
BSND CRISPRa kit - CRISPR gene activation of human barttin CLCNK type accessory beta subunit
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
BSND (untagged)-Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-XL6 |
Barttin (54-320, His-tag) human protein, 20 µg
Tag | His-tag |
Expression Host | E. coli |
Barttin (54-320, His-tag) human protein, 0.1 mg
Tag | His-tag |
Expression Host | E. coli |
BSND HEK293T cell transient overexpression lysate (as WB positive control)
Tag | C-Myc/DDK |
Expression Host | HEK293T |
Transient overexpression lysate of Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)
Tag | C-Myc/DDK |
Expression Host | HEK293T |
Lenti ORF clone of Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND), Myc-DDK-tagged
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
BSND (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene BSND
Application | Plasmid of exact quantity for transcript copy number calculation |
BSND - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
BSND - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |