United States
Germany
Japan
United Kingdom
China
AKT1S1 CRISPRa kit - CRISPR gene activation of human AKT1 substrate 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
AKT1S1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
AKT1S1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
AKT1S1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
qPCR primer pairs and template standards against Homo sapiens gene AKT1S1
| Application | Plasmid of exact quantity for transcript copy number calculation |
AKT1S1 (untagged) - Human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 4
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
3' UTR clone of AKT1 substrate 1 (proline-rich) (AKT1S1) transcript variant 3 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
AKT1S1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
AKT1S1 (untagged) - Human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 5
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
3' UTR clone of AKT1 substrate 1 (proline-rich) (AKT1S1) transcript variant 2 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene AKT1S1
