Products

ACTG1 CRISPRa kit - CRISPR gene activation of human actin gamma 1

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

ACTG1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

ACTG1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pGFP-V-RS

ACTG1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

qPCR primer pairs and template standards against Homo sapiens gene ACTG1

Application Plasmid of exact quantity for transcript copy number calculation

ACTG1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti

3' UTR clone of actin gamma 1 (ACTG1) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase