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qPCR primer pairs and template standards against Homo sapiens gene AKR1C3
| Application | Plasmid of exact quantity for transcript copy number calculation |
Products
AKR1C3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
3' UTR clone of aldo-keto reductase family 1 member C3 (3-alpha hydroxysteroid dehydrogenase type II) (AKR1C3) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
AKR1C3 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Recombinant Human Aldo-Keto Reductase 1C3/AKR1C3 (C-6His)
| Tag | C-His |
Transient overexpression of AKR1C3 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
