活动过滤器
clear Category
-
Proteins
- Recombinant Proteins
优化搜索结果
Target Symbol
- PD-L1 (36)
- PD1 (34)
- ALPHA 1 ANTITRYPSIN (32)
- MESOTHELIN (32)
- VEGFA (32)
- EPCAM (31)
- NCALD (31)
- TACD2 (29)
- HER2 (28)
- IRF5 (24)
- NDRG2 (24)
- FGF1 (22)
- ISOCITRATE DEHYDROGENASE (22)
- SNCA (22)
- ALKALINE PHOSPHATASE (21)
- ALPHA SYNUCLEIN (21)
- CD22 (21)
- FGFR1 (21)
- IL2 (21)
- IL6 (21)
- PQBP1 (21)
- RIBONUCLEASE INHIBITOR (21)
- TNF ALPHA (21)
- UBE2D3 (21)
- CPNE1 (20)
- HMGA1 (20)
- MAX (20)
- MORTALITY FACTOR 4 LIKE 2 (20)
- TPM1 (20)
- BCMA (19)
- CD7 (19)
- EGFR (19)
- IGF1 (19)
- MTH1 (19)
- AURORA A (18)
- CD19 (18)
- CD30 (18)
- CD47 (18)
- ICAM2 (18)
- IL4 (18)
- MYOSIN (18)
- PRKAR1B (18)
- UBE2I (18)
- CD137 (17)
- FSTL1 (17)
- IL8 (17)
- M-CSF (17)
- S100 CALCIUM BINDING PROTEIN A13 (17)
- SULT1A1 (17)
- ALDOLASE (16)
- BETA 2 MICROGLOBULIN (16)
- CATHEPSIN B (16)
- CD38 (16)
- CD3E (16)
- CTLA4 (16)
- FHL1 (16)
- GALE (16)
- GLUTAMINE SYNTHETASE (16)
- GM CSF (16)
- HMG1 (16)
- MICA (16)
- MYELIN BASIC PROTEIN (16)
- NDUF3 (16)
- PCBP2 (16)
- RNA5-8SN2 (16)
- TROPOMYOSIN 3 (16)
- 14-3-3 ZETA (15)
- ARSA (15)
- ASPARTATE BETA HYDROXYLASE (15)
- B3GALT5 (15)
- CD16 (15)
- CD70 (15)
- CEACAM1 (15)
- CUTA (15)
- DYNLL1 (15)
- ERBB 3 (15)
- FHL2 (15)
- FKBP2 (15)
- GLYPICAN 3 (15)
- HNF 4 ALPHA (15)
- HNRNP F (15)
- MBNL1 (15)
- P38 (15)
- PCSK9 (15)
- ACRV1 (14)
- AKT1 (14)
- ANNEXIN A2 (14)
- BDNF (14)
- C4BPB (14)
- CARBONIC ANHYDRASE X (14)
- CD36 (14)
- ESTROGEN RELATED RECEPTOR GAMMA (14)
- FBP1 (14)
- FGL1 (14)
- G CSF (14)
- GMPR2 (14)
- HOMER2 (14)
- IL1F10 (14)
- IL1RA (14)
- IL22 (14)
Expression Hosts
Tags
- C-Myc/DDK (27868)
- His-tag (5455)
- C-His (3467)
- N-His (2804)
- Tag Free (698)
- N-GST and C-His (441)
- C-Fc (327)
- C-6His (202)
- C-DDK (195)
- N-GST and C-HIS (150)
- N-GST (111)
- C-DDK/His (102)
- C-His-Avi (68)
- C-FLAG (58)
- Myc-DDK (57)
- C-Fc&His (52)
- N-His-ABP (Albumin-Binding Protein) (52)
- N-His-SUMO (49)
- C-Fc-Avi (48)
- C-HIS (45)
- C-Human Fc (44)
- C-mFc (43)
- N-6His (43)
- N-His&C-His (42)
- C-6His-Avi (36)
- His-Tag (32)
- C-Mouse Fc and 6×His (30)
- T7-tag (30)
- C-8His (24)
- N-T7&C-His (24)
- N-Trx&His (24)
- C-Mouse Fc (21)
- N-His-Trx (18)
- C-hFc (17)
- C-6×His (15)
- C-Avi-6His (14)
- C-Human Fc and 6×His (14)
- N-GST, C-His (13)
- C-10His (12)
- N-Fc (10)
- hIgG-His-tag (10)
- C-MYC/DDK (9)
- N-His&SUMO (9)
- N-GST&His (8)
- C-Avi&6His (7)
- Tag-free (7)
- C- 6×His (6)
- hIgG-Tag (6)
- C-Fc&AVI (5)
- N-Avi (5)
- N-HIS (5)
- N-His PDCD1(Pro21-Val170) (5)
- N-Mouse Fc and C-6×His (5)
- C-6xHis (4)
- His-Cam-tag (4)
- N-8His (4)
- N-His&C-Fc (4)
- N-His&T7 (4)
- N-His-GST (4)
- N-His/DDK (4)
- N-MARI (4)
- N-Trx (4)
- C-Fc/His (3)
- C-hFc&His (3)
- hIgG-His-Tag (3)
- C-Avi&His (2)
- C-Flag (2)
- C-HUMAN (2)
- CaM-tag (2)
- Calmodulin-tag (2)
- His-MBP-tag (2)
- Myc-His-tag (2)
- N-6His-Avi (2)
- N-DDK (2)
- N-GFP (2)
- N-His&Flag (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-Human Fc (2)
- N-TrxA (2)
- N-mFc (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- TrxA (2)
- C-6xHis/AVI (1)
- C-AVI (1)
- C-AVI&His (1)
- C-AVI-PLUS (1)
- C-Avi (1)
- C-Avi-10His (1)
- C-Avi-His (1)
- C-DDK-His (1)
- C-DDK/10xHis (1)
- C-DDK/6xHis (1)
- C-FC (1)
- C-FC-6HIS&C-FC-Flag (1)
- C-FLAG-6His (1)
- C-Fc-Flag&C-Fc-6His (1)
- C-Flag,C-6His (1)
- C-GST (1)
- C-HIS/C (1)
- C-HIS/DDK (1)
- C-His&AVI (1)
- C-His&Flag (1)
- C-His&HA&AVI (1)
- C-His/DDK (1)
- C-Human Fc, Avi (1)
- C-Human Fc-Avi (1)
- C-hIgG1 Fc (1)
- C-hIgG2 Fc (1)
- C-hIgG4 Fc (1)
- C-hIgG4P Fc (1)
- C-mFC (1)
- C-mIgG2a Fc (1)
- C-mIgG2aFc (1)
- C-terminal DDK (1)
- C-terminal His (1)
- Fc chimera (1)
- N-10His-Flag (1)
- N-6His&Avi (1)
- N-6His, C-6His (1)
- N-6His-FLAG (1)
- N-6His-Flag (1)
- N-8His&N-FLAG (1)
- N-8His-Flag (1)
- N-AVI (1)
- N-AVI&His (1)
- N-Avi&6His (1)
- N-DDK/His (1)
- N-FC (1)
- N-FLAG (1)
- N-FUSION (1)
- N-Fc-FLAG (1)
- N-Flag&C-His (1)
- N-Flag&His (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-Mouse Fc (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
- N-hFc (1)
- No (1)
- Tag free (1)
Conjugates
Mutants
Brands
QC Levels
Families
- Druggable Genome (19854)
- Transmembrane (7921)
- Secreted Protein (4748)
- Transcription Factors (4107)
- Protein Kinase (1695)
- ES Cell Differentiation/IPS (1552)
- Protease (1305)
- Stem cell - Pluripotency (1215)
- Phosphatase (544)
- Adult stem cells (346)
- Ion Channels: Other (262)
- Embryonic stem cells (259)
- Cancer stem cells (179)
- Nuclear Hormone Receptor (171)
- Induced pluripotent stem cells (152)
- P450 (134)
- GPCR (122)
- Stem cell relevant signaling - JAK/STAT signaling pathway (119)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (83)
- Stem cell relevant signaling - Wnt Signaling pathway (71)
- Stem cell relevant signaling - DSL/Notch pathway (64)
- Ion Channels: Potassium (40)
- Ion Channels: Cys-loop Receptors (19)
- Ion Channels: Glutamate Receptors (19)
- Ion Channels: ATP Receptors (13)
- Ion Channels: Transient receptor potential (13)
- Ion Channels: Sodium (10)
- ELISA (3)
-
ELISA.
Western Blot. (3) - FACS (3)
- Ion Channels: Cyclic nucleotide gated (3)
- ELISA. (2)
-
ELISA.
Western blot.
Lateral flow. (2) - Suitable for use in ELISA. (2)
-
0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes. (1) -
0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug (1) - 0.1 mg/ml Ovalbumin (1)
- 0.1% (w/v) insulin (1)
- 0.12 mM beta-nicotinamide adenine dinucleotide (1)
- 0.5 mM EDTA (1)
-
0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes. (1) - 0.9 mM Xanthine (1)
- 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl (1)
- 1 mM DTT (1)
- 10 mM Tris-HCl (1)
-
10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug (1) - 1mM DTT (1)
- 1mM MnCl2 (1)
- 2 mM dithiothreitol (1)
- 2.3 mM Pyruvate (1)
- 2mM DTT (1)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (1) -
3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm. (1) - 5.5 mM Ethylendiaminetetraacetic acid (1)
- 50mM NaCl (1)
- 63mM sodium phosphate (1)
-
655077)
- Fluorescent plate reader (PerkinElmer (1) - 66 mM Cytochrom-C (1)
-
ELISA (0.5 µg/ml).
Western blot. (1) - ELISA. (1)
-
ELISA.
Western Blot.
Lateral Flow. (1) -
ELISA.
Western Blot. (1) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) -
Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard. (1) -
BML-SE211)
- 96 Well Polystyrene Microplate (1) - Cat.No (1)
- Citric acid 0.055 g (1)
-
D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination. (1) - ELISA and Western blot. (1)
-
ELISA.
Western blot. (1) - Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng (1)
-
Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate (1) -
Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container (1) -
Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5) (1) -
Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl (1) -
Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g (1) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (1) - Sodium citric aci d 0.8g (1)
- Suitable for ELISA and Western blot. (1)
- Suitable for use in ELISA and Western blot. (1)
- Suitable for use in Western blot. (1)
- VICTOR X3) (1)
- adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate (1)
-
and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C. (1) - black (greiner bio-one (1)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1)
- e.g. Dulbecco's PBS). (1)
-
pH 7.4).
For immunization purposes (1) - pH 8.0 (1)
-
pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo (1) - reduced form (1)
- the solution can be further dialyzed against PBS (phosphate buffered saline (1)
Pathways
- Metabolic pathways (3762)
- Cytokine-cytokine receptor interaction (1642)
- Pathways in cancer (1582)
- MAPK signaling pathway (1271)
- Focal adhesion (892)
- Regulation of actin cytoskeleton (879)
- Chemokine signaling pathway (804)
- Jak-STAT signaling pathway (775)
- Endocytosis (727)
- Natural killer cell mediated cytotoxicity (655)
- Neurotrophin signaling pathway (620)
- T cell receptor signaling pathway (619)
- Hematopoietic cell lineage (604)
- Purine metabolism (584)
- Toll-like receptor signaling pathway (576)
- Alzheimer's disease (564)
- Ubiquitin mediated proteolysis (543)
- Cell adhesion molecules (CAMs) (540)
- Insulin signaling pathway (540)
- Axon guidance (486)
- Huntington's disease (485)
- Cell cycle (478)
- Wnt signaling pathway (477)
- Leukocyte transendothelial migration (467)
- ErbB signaling pathway (464)
- Prostate cancer (461)
- Apoptosis (454)
- Pancreatic cancer (442)
- Melanoma (427)
- Pyrimidine metabolism (424)
- Tight junction (423)
- Progesterone-mediated oocyte maturation (415)
- Fc epsilon RI signaling pathway (407)
- Oocyte meiosis (403)
- Spliceosome (403)
- Renal cell carcinoma (399)
- Adherens junction (391)
- Colorectal cancer (391)
- GnRH signaling pathway (384)
- TGF-beta signaling pathway (378)
- Antigen processing and presentation (370)
- Lysosome (370)
- NOD-like receptor signaling pathway (370)
- Calcium signaling pathway (365)
- Parkinson's disease (365)
- RIG-I-like receptor signaling pathway (361)
- Fc gamma R-mediated phagocytosis (360)
- Hypertrophic cardiomyopathy (HCM) (355)
- B cell receptor signaling pathway (353)
- Chronic myeloid leukemia (350)
- Glioma (347)
- Glycolysis / Gluconeogenesis (344)
- VEGF signaling pathway (344)
- Dilated cardiomyopathy (338)
- Epithelial cell signaling in Helicobacter pylori infection (324)
- Systemic lupus erythematosus (323)
- p53 signaling pathway (317)
- Adipocytokine signaling pathway (315)
- Small cell lung cancer (313)
- Bladder cancer (312)
- PPAR signaling pathway (311)
- Gap junction (306)
- Complement and coagulation cascades (295)
- Melanogenesis (287)
- Cytosolic DNA-sensing pathway (285)
- Acute myeloid leukemia (281)
- Vascular smooth muscle contraction (277)
- Arginine and proline metabolism (274)
- Type I diabetes mellitus (268)
- Endometrial cancer (267)
- Long-term potentiation (267)
- Non-small cell lung cancer (267)
- Oxidative phosphorylation (263)
- Amyotrophic lateral sclerosis (ALS) (261)
- Autoimmune thyroid disease (256)
- Pathogenic Escherichia coli infection (256)
- mTOR signaling pathway (256)
- Viral myocarditis (248)
- Glutathione metabolism (239)
- Long-term depression (238)
- Metabolism of xenobiotics by cytochrome P450 (238)
- Phosphatidylinositol signaling system (237)
- Amino sugar and nucleotide sugar metabolism (236)
- Drug metabolism - cytochrome P450 (227)
- Allograft rejection (222)
- Graft-versus-host disease (216)
- ECM-receptor interaction (212)
- Fructose and mannose metabolism (202)
- Cardiac muscle contraction (201)
- Type II diabetes mellitus (198)
- Tyrosine metabolism (196)
- Ribosome (195)
- Valine (195)
- Proteasome (194)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (192)
- Inositol phosphate metabolism (191)
- Prion diseases (189)
- Neuroactive ligand-receptor interaction (187)
- Drug metabolism - other enzymes (184)
- leucine and isoleucine degradation (182)
- Thyroid cancer (180)
- Glycerophospholipid metabolism (178)
- RNA degradation (176)
- Cysteine and methionine metabolism (173)
- Pyruvate metabolism (173)
- Tryptophan metabolism (168)
- Alanine (166)
- Regulation of autophagy (166)
- aspartate and glutamate metabolism (166)
- Hedgehog signaling pathway (165)
- Retinol metabolism (165)
- Fatty acid metabolism (164)
- Starch and sucrose metabolism (162)
- Vibrio cholerae infection (159)
- Pentose phosphate pathway (158)
- Base excision repair (155)
- Primary immunodeficiency (155)
- Arachidonic acid metabolism (149)
- Nitrogen metabolism (149)
- Asthma (145)
- Porphyrin and chlorophyll metabolism (144)
- Androgen and estrogen metabolism (139)
- Glycerolipid metabolism (138)
- Nucleotide excision repair (136)
- SNARE interactions in vesicular transport (133)
- Galactose metabolism (131)
- Butanoate metabolism (130)
- Citrate cycle (TCA cycle) (128)
- Propanoate metabolism (121)
- Basal cell carcinoma (116)
- DNA replication (115)
- Glycine (115)
- serine and threonine metabolism (115)
- Aminoacyl-tRNA biosynthesis (114)
- Lysine degradation (113)
- Histidine metabolism (112)
- beta-Alanine metabolism (107)
- Nicotinate and nicotinamide metabolism (106)
- Sphingolipid metabolism (105)
- Phenylalanine metabolism (101)
- Olfactory transduction (100)
- Notch signaling pathway (96)
- RNA polymerase (94)
- Dorso-ventral axis formation (93)
- Selenoamino acid metabolism (87)
- Maturity onset diabetes of the young (86)
- Sulfur metabolism (86)
- Ether lipid metabolism (85)
- Glyoxylate and dicarboxylate metabolism (82)
- Homologous recombination (80)
- Mismatch repair (77)
- Riboflavin metabolism (77)
- Linoleic acid metabolism (76)
- Pentose and glucuronate interconversions (76)
- Terpenoid backbone biosynthesis (75)
- Pantothenate and CoA biosynthesis (72)
- Basal transcription factors (71)
- Other glycan degradation (70)
- Renin-angiotensin system (70)
- Glycosaminoglycan degradation (68)
- N-Glycan biosynthesis (64)
- One carbon pool by folate (62)
- Ascorbate and aldarate metabolism (61)
- Glycosphingolipid biosynthesis - lacto and neolacto series (57)
- Folate biosynthesis (53)
- Primary bile acid biosynthesis (50)
- Taste transduction (50)
- C21-Steroid hormone metabolism (48)
- Limonene and pinene degradation (47)
- alpha-Linolenic acid metabolism (44)
- Glycosphingolipid biosynthesis - globo series (43)
- Synthesis and degradation of ketone bodies (43)
- O-Glycan biosynthesis (40)
- Thiamine metabolism (40)
- Biosynthesis of unsaturated fatty acids (37)
- Methane metabolism (37)
- Non-homologous end-joining (35)
- Heparan sulfate biosynthesis (33)
- Keratan sulfate biosynthesis (32)
- Circadian rhythm - mammal (31)
- Steroid biosynthesis (31)
- ABC transporters (29)
- Fatty acid elongation in mitochondria (28)
- Taurine and hypotaurine metabolism (28)
- Glycosphingolipid biosynthesis - ganglio series (26)
- Ubiquinone and other terpenoid-quinone biosynthesis (25)
- Chondroitin sulfate biosynthesis (24)
- Phenylalanine (24)
- leucine and isoleucine biosynthesis (24)
- tyrosine and tryptophan biosynthesis (24)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (23)
- Protein export (22)
- Caffeine metabolism (20)
- Vitamin B6 metabolism (20)
- Fatty acid biosynthesis (15)
- Cyanoamino acid metabolism (14)
- Lysine biosynthesis (14)
- D-Glutamine and D-glutamate metabolism (13)
- D-Arginine and D-ornithine metabolism (8)
- Biotin metabolism (6)
- O-Mannosyl glycan biosynthesis (4)
- Lipoic acid metabolism (1)