VPS35 CRISPRa kit - CRISPR gene activation of human VPS35 retromer complex component
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
VPS35 CRISPRa kit - CRISPR gene activation of human VPS35 retromer complex component
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
Tag | C-Myc/DDK |
Expression Host | HEK293T |
VPS35 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene VPS35
Application | Plasmid of exact quantity for transcript copy number calculation |
VPS35 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
3' UTR clone of vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) for miRNA target validation
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene VPS35
Recombinant Human VPS35 Protein
Tag | C-His |
Transient overexpression of VPS35 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack