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VARS2 CRISPRa kit - CRISPR gene activation of human valyl-tRNA synthetase 2, mitochondrial
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
VARS2 MS Standard C13 and N15-labeled recombinant protein (NP_065175)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
Transient overexpression lysate of valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Lenti-ORF clone of VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 3
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti-ORF clone of VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qPCR primer pairs and template standards against Homo sapiens gene VARS2
| Application | Plasmid of exact quantity for transcript copy number calculation |
VARS2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
VARS2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
VARS2 (untagged)-Human valyl-tRNA synthetase 2 mitochondrial (putative) (VARS2) transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
VARS2 (untagged)-Human valyl-tRNA synthetase 2 mitochondrial (putative) (VARS2) transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
3' UTR clone of valyl-tRNA synthetase 2 mitochondrial (putative) (VARS2) nuclear gene encoding mitochondrial protein for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene VARS2
VARS2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
