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Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1, mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Products
PPEF1 CRISPRa kit - CRISPR gene activation of human protein phosphatase with EF-hand domain 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
PPEF1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PPEF1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Lenti-ORF clone of PPEF1 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 3
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1, mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP |
- LentiORF®
PPEF1 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
PPEF1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene PPEF1
| Application | Plasmid of exact quantity for transcript copy number calculation |
3' UTR clone of protein phosphatase EF-hand calcium binding domain 1 (PPEF1) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
3' UTR clone of protein phosphatase EF-hand calcium binding domain 1 (PPEF1) transcript variant 1b for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
PPEF1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
PPEF1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
PPEF1 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1b
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
