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GRK5 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
Products
GRK5 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
GRK5 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
GRK5 CRISPRa kit - CRISPR gene activation of human G protein-coupled receptor kinase 5
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
GRK5 MS Standard C13 and N15-labeled recombinant protein (NP_005299)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
GRK5 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
GRK5 (untagged)-Kinase deficient mutant (K215M) of Human G protein-coupled receptor kinase 5 (GRK5)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
GRK5 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
GRK5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
qPCR primer pairs and template standards against Homo sapiens gene GRK5
| Application | Plasmid of exact quantity for transcript copy number calculation |
GRK5 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
3' UTR clone of G protein-coupled receptor kinase 5 (GRK5) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene GRK5
Transient overexpression of GRK5 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
