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FADD CRISPRa kit - CRISPR gene activation of human Fas associated via death domain
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
FADD MS Standard C13 and N15-labeled recombinant protein (NP_003815)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
FADD (1-208, His-tag) human recombinant protein, 0.25 mg
| Tag | His-tag |
| Expression Host | E. coli |
FADD - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
FADD - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
FADD (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SR322535 is the updated version of SR305777.
3' UTR clone of Fas (TNFRSF6)-associated via death domain (FADD) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Transient overexpression of FADD in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
qSTAR qPCR primer pairs against Homo sapiens gene FADD
