E2F1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
E2F1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
E2F1 - human gene knockout kit via CRISPR, HDR mediated
Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
Donor DNA | mBFP-Neo |
E2F1 - human gene knockout kit via CRISPR, HDR mediated
Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
Donor DNA | Luciferase-Puro |
E2F1 - human gene knockout kit via CRISPR, HDR mediated
Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
Donor DNA | RFP-BSD |
E2F1 CRISPRa kit - CRISPR gene activation of human E2F transcription factor 1
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
E2F1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |
E2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005216)
Tag | C-Myc/DDK |
Expression Host | HEK293 |
E2F1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
3' UTR clone of E2F transcription factor 1 (E2F1) for miRNA target validation
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
qPCR primer pairs and template standards against Homo sapiens gene E2F1
Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene E2F1
Transient overexpression of E2F1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack