Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Lentiviral Particles

Over-expression Lysates

Native Proteins

cDNA Clone Sets

Human

Mouse

Humon

RET

FGFR1

BDNF

NRG1

MU OPIOID RECEPTOR

EMA

ASPARTATE BETA HYDROXYLASE

EGFR

CACNA1G

ST3GAL3

UTY

CD46

CACNA1C

CD1E

FGFR2

GPR56

ALPHA DYSTROGLYCAN

GM CSF RECEPTOR ALPHA

HFE

AMYLOID PRECURSOR PROTEIN

CD89

ZFYVE27

CD36

CD44

FXYD3

MYELIN OLIGODENDROCYTE GLYCOPROTEIN

TRKB

FLT4

MYADM

MCK10

TMPRSS4

B3GALT5

CEACAM1

CHRM2

C18ORF1

HEME OXYGENASE 2

CD299

GPR64

IL5RA

NEUROLIGIN 4

NRP2

P2X2

CADHERIN LIKE 23

CD133

ICAM2

M-CSF

MTLRP

PTGER3

BAX

CD32B

GLEPP1

HER2

LINGO1

OS9

PGAP2

SYNTENIN

CD10

IL1RAP

MACROPHAGE INFLAMMATORY PROTEIN 1 BETA

SLAMF7

CD39

CUTA

DYSFERLIN

FCRLM1

GABA A RECEPTOR ALPHA 1

NOGO A

SIGLEC10

TRPM3

TSPAN4

AIF

BCL RAMBO

CD147

ELASTIN

RAG1AP1

SEZ6L

SLITRK2

STRA6

TRKC

VRK2

ADAM15

CCK4

P2Y6

PAQR6

SEMA6D

ASIALOGLYCOPROTEIN RECEPTOR 2

ATP2C1

CMTM1

FXYD6

GDNF

GROWTH HORMONE RECEPTOR

LILRB1

MIDKINE

CD16

CD98

KCNMA1

LDL RECEPTOR

NEURONAL MEMBRANE GLYCOPROTEIN M6 A

NMDAR1

RAGE

SERCA3

IHC

WB

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Human

Plasmids

Particles

Myc-DDK

Tag Free

mGFP

TurboGFP

C-Myc/DDK

C-MYC/DDK

C-His

N-His

C-Fc

C-DDK/His

C-6His

N-GST and C-His

DDK-His

C-DDK

N-GST and C-HIS

C-mFc

C-Fc&His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

N-Trx&His

C-HIS

C-6×His

C-Fc-Avi

C-Human Fc

C-hFc

His-tag

C-Mouse Fc

C-8His

N-6His

N-His-Trx

N-GST

C-Mouse Fc and 6×His

C-Avi-6His

N-Fc

C- 6×His

C-10His

C-Fc&AVI

C-Human Fc and 6×His

N-Trx

C-Fc/His

C-hFc&His

N-GST, C-His

N-His/DDK

C-Flag

C-HUMAN

N-6His-Avi

N-Mouse Fc and C-6×His

Tag-free

C-6xHis/AVI

C-AVI&His

C-FC

C-FC-6HIS&C-FC-Flag

C-GST

C-His/DDK

C-hIgG2 Fc

C-hIgG4 Fc

C-mFC

N-6His-Flag

N-8His

N-8His&N-FLAG

N-Avi&6His

N-DDK/His

N-FC

N-Flag&His

N-GFP

N-His PDCD1(Pro21-Val170)

N-His-GST

N-TRX

N-TRXA

N-Trx-His

N-mFc

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

pCMV6-AC-Myc-DDK

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Metabolic pathways

Neuroactive ligand-receptor interaction

Cytokine-cytokine receptor interaction

Cell adhesion molecules (CAMs)

Olfactory transduction

Pathways in cancer

Calcium signaling pathway

Hematopoietic cell lineage

MAPK signaling pathway

Endocytosis

Regulation of actin cytoskeleton

Jak-STAT signaling pathway

Natural killer cell mediated cytotoxicity

Alzheimer's disease

Dilated cardiomyopathy

Axon guidance

Focal adhesion

Hypertrophic cardiomyopathy (HCM)

Lysosome

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Adherens junction

Antigen processing and presentation

ECM-receptor interaction

Chemokine signaling pathway

Leukocyte transendothelial migration

Vascular smooth muscle contraction

Viral myocarditis

Huntington's disease

N-Glycan biosynthesis

Prostate cancer

Oxidative phosphorylation

Systemic lupus erythematosus

ABC transporters

Colorectal cancer

Drug metabolism - cytochrome P450

Melanoma

Retinol metabolism

Cardiac muscle contraction

Adipocytokine signaling pathway

Graft-versus-host disease

Parkinson's disease

ErbB signaling pathway

Autoimmune thyroid disease

Melanogenesis

SNARE interactions in vesicular transport

Apoptosis

PPAR signaling pathway

Type I diabetes mellitus

Allograft rejection

Glycosphingolipid biosynthesis - lacto and neolacto series

Epithelial cell signaling in Helicobacter pylori infection

Toll-like receptor signaling pathway

Metabolism of xenobiotics by cytochrome P450

Neurotrophin signaling pathway

Purine metabolism

Gap junction

Tight junction

GnRH signaling pathway

TGF-beta signaling pathway

Glycerophospholipid metabolism

Pancreatic cancer

Starch and sucrose metabolism

Drug metabolism - other enzymes

Amyotrophic lateral sclerosis (ALS)

T cell receptor signaling pathway

Androgen and estrogen metabolism

Sphingolipid metabolism

Wnt signaling pathway

O-Glycan biosynthesis

Primary immunodeficiency

Basal cell carcinoma

Porphyrin and chlorophyll metabolism

Type II diabetes mellitus

Taste transduction

Asthma

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Long-term potentiation

Arachidonic acid metabolism

Keratan sulfate biosynthesis

Vibrio cholerae infection

Thyroid cancer

Long-term depression

Heparan sulfate biosynthesis

Bladder cancer

Fatty acid metabolism

B cell receptor signaling pathway

Complement and coagulation cascades

Fc gamma R-mediated phagocytosis

Hedgehog signaling pathway

Ascorbate and aldarate metabolism

p53 signaling pathway

Pentose and glucuronate interconversions

Ether lipid metabolism

Notch signaling pathway

Pyrimidine metabolism

Chondroitin sulfate biosynthesis

Glycosphingolipid biosynthesis - globo series

Linoleic acid metabolism

Endometrial cancer

Glioma

Glycerolipid metabolism

Renin-angiotensin system

Tyrosine metabolism

Non-small cell lung cancer

Fc epsilon RI signaling pathway

Glutathione metabolism

Glycosphingolipid biosynthesis - ganglio series

Prion diseases

Small cell lung cancer

Chronic myeloid leukemia

Steroid biosynthesis

Renal cell carcinoma

Pathogenic Escherichia coli infection

Cytosolic DNA-sensing pathway

Riboflavin metabolism

Progesterone-mediated oocyte maturation

RIG-I-like receptor signaling pathway

Biosynthesis of unsaturated fatty acids

Nicotinate and nicotinamide metabolism

Insulin signaling pathway

Oocyte meiosis

Glycosaminoglycan degradation

NOD-like receptor signaling pathway

Dorso-ventral axis formation

Galactose metabolism

Ubiquitin mediated proteolysis

VEGF signaling pathway

Primary bile acid biosynthesis

alpha-Linolenic acid metabolism

Phosphatidylinositol signaling system

Amino sugar and nucleotide sugar metabolism

Inositol phosphate metabolism

Nitrogen metabolism

Tryptophan metabolism

C21-Steroid hormone metabolism

Cyanoamino acid metabolism

Selenoamino acid metabolism

Taurine and hypotaurine metabolism

Caffeine metabolism

Acute myeloid leukemia

beta-Alanine metabolism

Phenylalanine metabolism

Glycine

serine and threonine metabolism

Glycolysis / Gluconeogenesis

Propanoate metabolism

Valine

leucine and isoleucine degradation

Protein export

Sulfur metabolism

Limonene and pinene degradation

Cell cycle

Lysine degradation

O-Mannosyl glycan biosynthesis

Arginine and proline metabolism

Histidine metabolism

Other glycan degradation

Pantothenate and CoA biosynthesis

Pyruvate metabolism

Citrate cycle (TCA cycle)

Butanoate metabolism

Base excision repair

Fatty acid elongation in mitochondria

Terpenoid backbone biosynthesis

Maturity onset diabetes of the young

Ubiquinone and other terpenoid-quinone biosynthesis

Fatty acid biosynthesis

Human

Human 293

E. coli

CHO Stable

HEK293

Mammalian

Neutrophils

P.Pichia

Plasma

Neomycin

Puromycin

None

1

2

Yes

In Stock