Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Over-expression Lysates

Mutant Proteins

cDNA Clone Sets

Lentiviral Particles

Human

BRCA1

MENIN

P53

CREM

NRG1

MORTALITY FACTOR 4 LIKE 2

WILMS TUMOR PROTEIN

CONSTITUTIVE ANDROSTANE RECEPTOR

BETA CATENIN

IKAROS

IRF5

PLAGL1

HNF 4 ALPHA

ESTROGEN RELATED RECEPTOR GAMMA

PQBP1

ATAXIN 3

TGIF

RUNX1T1

HMGA1

TCF7L2

GLUCOCORTICOID RECEPTOR

PML PROTEIN

MAX

MEIS2

PRKCBP1

UBE2V1

P73

CAMKK2

MBD1

TCF4

MITF

ZNF133

BORIS

NFAT2

PAX3

XPD

ZNF302

P63

PPAR GAMMA

ZNF83

ERG

NFAT1

PTGER3

ESTROGEN RECEPTOR 1

GABPB2

HNRNPD

IKK GAMMA

RNF14

C-MYB

ER81

FE65

HOPX

NCOA4

HDAC9

RBFOX2

BMAL1

STAU2

VGL4

ZNF268

DYNAMIN 2

IRF3

NFYC

NONO

PAX5

PROTEIN KINASE A REGULATORY SUBUNIT I ALPHA

ZBTB20

ATF3

CNBP

EVI1

ZINC FINGER PROTEIN 655

APE1

RHOH

SCMH1

SUPT5H

CALCIPRESSIN 1

ESTROGEN RECEPTOR BETA

HEXIM2

ING4

RBPJK

EWSR1

FOXP1

FUSIP1

GROWTH ARREST SPECIFIC PROTEIN 7

KAT5

PAX8

ZFP200

CARHSP1

CNOT4

EYA1

SPLICING FACTOR 1

CBL

DAXX

DP2

HDAC8

HIF1 BETA

LIM DOMAIN ONLY 3

NUP62

PITX2

SOD2

AREB6

IHC

WB

HEK293T

HEK293

E. coli

Sf9

CHO

Insect

Plasmids

Particles

Myc-DDK

Tag Free

TurboGFP

mGFP

C-Myc/DDK

C-MYC/DDK

N-His

N-GST and C-His

C-His

C-DDK

N-GST and C-HIS

N-GST

His-tag

N-His&SUMO

N-His-ABP (Albumin-Binding Protein)

N-MARI

N-6His

C-DDK/His

N-GST, C-His

N-His-GST

C-6×His

C-Fc

C-Fc/His

C-Mouse Fc and 6×His

C-mFc

N-6His-FLAG

N-Avi

N-His&Flag

N-His/DDK

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pCMV6-XL5

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL4

pCMV6-AC

pCMV6-AC-Myc-DDK

pCMV6-XL6

PCMV6-Neo

pCMV6-AN-Myc-DDK

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Pathways in cancer

MAPK signaling pathway

Wnt signaling pathway

Ubiquitin mediated proteolysis

Prostate cancer

Cell cycle

Huntington's disease

Chronic myeloid leukemia

Thyroid cancer

Acute myeloid leukemia

Small cell lung cancer

Neurotrophin signaling pathway

Pancreatic cancer

Colorectal cancer

Metabolic pathways

TGF-beta signaling pathway

T cell receptor signaling pathway

Endometrial cancer

Apoptosis

Basal cell carcinoma

Toll-like receptor signaling pathway

Purine metabolism

Pyrimidine metabolism

B cell receptor signaling pathway

Basal transcription factors

Adipocytokine signaling pathway

Melanogenesis

Non-small cell lung cancer

p53 signaling pathway

RNA polymerase

Cytosolic DNA-sensing pathway

Melanoma

Amyotrophic lateral sclerosis (ALS)

Adherens junction

Bladder cancer

Chemokine signaling pathway

Jak-STAT signaling pathway

Maturity onset diabetes of the young

Glioma

ErbB signaling pathway

Natural killer cell mediated cytotoxicity

RIG-I-like receptor signaling pathway

Axon guidance

Renal cell carcinoma

Endocytosis

Nucleotide excision repair

Spliceosome

VEGF signaling pathway

RNA degradation

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Tight junction

Insulin signaling pathway

PPAR signaling pathway

Oocyte meiosis

Neuroactive ligand-receptor interaction

Antigen processing and presentation

NOD-like receptor signaling pathway

Notch signaling pathway

Focal adhesion

Leukocyte transendothelial migration

Systemic lupus erythematosus

Alzheimer's disease

Epithelial cell signaling in Helicobacter pylori infection

Circadian rhythm - mammal

Pathogenic Escherichia coli infection

Viral myocarditis

Primary immunodeficiency

DNA replication

Allograft rejection

Graft-versus-host disease

Type I diabetes mellitus

Dorso-ventral axis formation

Fc gamma R-mediated phagocytosis

Base excision repair

Calcium signaling pathway

Cytokine-cytokine receptor interaction

Lysine degradation

Fc epsilon RI signaling pathway

Type II diabetes mellitus

GnRH signaling pathway

Autoimmune thyroid disease

Cell adhesion molecules (CAMs)

Long-term depression

Long-term potentiation

Regulation of actin cytoskeleton

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

Cysteine and methionine metabolism

mTOR signaling pathway

Hedgehog signaling pathway

Ribosome

Homologous recombination

Valine

Asthma

Hematopoietic cell lineage

Aminoacyl-tRNA biosynthesis

Non-homologous end-joining

Progesterone-mediated oocyte maturation

leucine and isoleucine biosynthesis

Ether lipid metabolism

Vascular smooth muscle contraction

Glycolysis / Gluconeogenesis

Glycerolipid metabolism

Glycine

Glyoxylate and dicarboxylate metabolism

Nicotinate and nicotinamide metabolism

Prion diseases

SNARE interactions in vesicular transport

serine and threonine metabolism

Proteasome

leucine and isoleucine degradation

Mismatch repair

Biosynthesis of unsaturated fatty acids

Human

E. coli

Human 293

Neomycin

Puromycin

None

1

2

Yes

In Stock