Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

Over-expression Lysates

CytoSections

Mass Spec Standards

Mutant ORF Clones

Native Proteins

Mutant Proteins

Lentiviral Particles

Human

Mouse

Chicken

Human, Rat

Porcine

Bovine

Hamster

BDNF

VEGFA

NRG1

ALPHA 1 ANTITRYPSIN

G PROTEIN ALPHA S

EGFR

EMA

FGF1

ST3GAL3

IL32

FGFR2

GM CSF RECEPTOR ALPHA

ALPHA DYSTROGLYCAN

GELSOLIN

RNASE9

M-CSF

HYAL1

IGF1

ANNEXIN A2

IL1RA

OSTEOPONTIN

DECORIN

KALLIKREIN 11

PACE4

IL1RAP

GDNF

IL18BP

FGL1

G CSF

MIDKINE

MTLRP

PERIOSTIN

SIGLEC6

CD16

SDF1

KALLIKREIN 6

PARATHYROID HORMONE RELATED PROTEIN

SEX HORMONE BINDING GLOBULIN

FGF13

NEUROPILIN 1

PLAUR

RAGE

SPAG11B

FCRLM1

CRISP2

GROWTH HORMONE

IL37

CALUMENIN

CD8B

FAM3A

HSD17B13

MAGP1

REG3A

APRIL

ERBB 3

IGF2

MTMR14

RAB37

RIBONUCLEASE A

SYNAPTOTAGMIN 1

TCTN1

AMCASE

CLUSTERIN

KALLIKREIN 8

NELL2

CALCITONIN

DKK3

ELASTIN

MESOTHELIN

APOLIPOPROTEIN E

BMP4

CD95

FOLATE BINDING PROTEIN

LIPF

NBL1

RNASE4

RTBDN

THROMBOPOIETIN

CDW75

CTRP1

FGF8

ICAM4

KALLIKREIN 5

REG3G

TRYPSIN

APOLIPOPROTEIN L 1

C2

CCL14

EFEMP1

IL4

LEPTIN RECEPTOR

SPOCK3

TNFRSF18

VAMP1

APOER2

FAM3C

IL1F10

VMO1

ARTEMIN

ARTS1

IHC

WB

HEK293T

E. coli

HEK293

Insect

CHO

Sf9

Pichia

Hi-5 insect

Plasmids

Particles

Myc-DDK

C-Myc/DDK

Tag Free

mGFP

TurboGFP

C-MYC/DDK

C-His

His-tag

N-His

C-6His

C-Fc

N-GST and C-His

N-GST and C-HIS

C-DDK

C-HIS

C-Fc&His

C-DDK/His

C-8His

N-GST

DDK-His

C-Human Fc

N-His-Trx

C-10His

C-mFc

N-6His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

Tag-free

hIgG-His-tag

N-Avi

C-hFc

N-Fc

T7-tag

C-Avi-6His

N-His/DDK

Calmodulin-tag

Myc-His-tag

N-8His

N-His-PDCD1(Pro21-Val170)

C-AVI

C-AVI&His

C-Avi

C-Avi&6His

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FLAG

C-Fc-Avi

C-Fc/His

C-Flag,C-6His

C-His&Flag

C-His&HA&AVI

C-Mouse Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-terminal His

N-6His&Avi

N-6His-Avi

N-AVI&His

N-DDK/His

N-FLAG

N-Fc-FLAG

N-Flag&His

N-HIS

N-His&C-His

N-His&Flag

N-T7

N-Trx-His

N-hFc

N-mFc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

Tag free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

Biotin

FITC

HRP

TRITC

Texas Red

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

WB

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

Induced pluripotent stem cells

P450

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

ELISA.
Western Blot.

FACS

Suitable for use in ELISA.

ELISA.

ELISA.

ELISA.
Western blot.
Lateral flow.

Immunogen

Secreted

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

000 or less. This should be performed in a cold room.

1 mM DTT

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

RIA.
Western blot.

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Apo AI can be renatured into PBS

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA and Western blot.

ELISA. Can be used for all immunological/laboratory methods requiring highly purified antigens.

ELISA.
Western blot.

ELISA: 0.4-0.8 µg/well.
After thawing

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Second order rate constants for inhibition of:
uPA = 6.3 x 10e6 M-1s-1
tPA = 5.7 x 10e6 M-1s-1

Sodium citric aci d 0.8g

Specific methodologies have not been tested using this product.

Suitable for ELISA and Western blot.

Suitable for use in ELISA and Western blot.

Suitable for use in Western blot.

Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces

TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14

VICTOR X3)

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

calibrator in immunoassays and in vitro studies.

calibrator in immunoassays and in vitro studies.

completely thaw the tube at 37°C without agitation

e.g. Dulbecco's PBS).

flicking or mixing

glassware and microcarrier beads. After spin and opening of the vial

including plasticware

mix thoroughly by vortexing before use!

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

then gently mix the contents with a pipette.

Cytokine-cytokine receptor interaction

Pathways in cancer

Jak-STAT signaling pathway

MAPK signaling pathway

Complement and coagulation cascades

Regulation of actin cytoskeleton

Chemokine signaling pathway

Hematopoietic cell lineage

Focal adhesion

Metabolic pathways

Melanoma

Toll-like receptor signaling pathway

TGF-beta signaling pathway

Natural killer cell mediated cytotoxicity

ECM-receptor interaction

Autoimmune thyroid disease

GnRH signaling pathway

Melanogenesis

Pancreatic cancer

Hedgehog signaling pathway

Bladder cancer

Wnt signaling pathway

Neuroactive ligand-receptor interaction

Cytosolic DNA-sensing pathway

Systemic lupus erythematosus

RIG-I-like receptor signaling pathway

Basal cell carcinoma

Dilated cardiomyopathy

Type I diabetes mellitus

Allograft rejection

Antigen processing and presentation

Prostate cancer

Endocytosis

ErbB signaling pathway

Renal cell carcinoma

T cell receptor signaling pathway

mTOR signaling pathway

NOD-like receptor signaling pathway

Long-term depression

Hypertrophic cardiomyopathy (HCM)

Axon guidance

Fc epsilon RI signaling pathway

VEGF signaling pathway

Apoptosis

Graft-versus-host disease

Calcium signaling pathway

Asthma

Prion diseases

PPAR signaling pathway

Regulation of autophagy

Gap junction

Neurotrophin signaling pathway

Vascular smooth muscle contraction

Adipocytokine signaling pathway

p53 signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Alzheimer's disease

Glioma

Small cell lung cancer

Colorectal cancer

Primary immunodeficiency

Huntington's disease

Cell adhesion molecules (CAMs)

Arachidonic acid metabolism

Ether lipid metabolism

Viral myocarditis

Fc gamma R-mediated phagocytosis

Glycosphingolipid biosynthesis - lacto and neolacto series

Glycerophospholipid metabolism

Glycerolipid metabolism

Non-small cell lung cancer

Keratan sulfate biosynthesis

Renin-angiotensin system

Taste transduction

Vibrio cholerae infection

Glycosaminoglycan degradation

Linoleic acid metabolism

alpha-Linolenic acid metabolism

Lysosome

Type II diabetes mellitus

Adherens junction

Dorso-ventral axis formation

Endometrial cancer

Cell cycle

Oocyte meiosis

Progesterone-mediated oocyte maturation

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Amyotrophic lateral sclerosis (ALS)

Chronic myeloid leukemia

SNARE interactions in vesicular transport

Amino sugar and nucleotide sugar metabolism

O-Glycan biosynthesis

Leukocyte transendothelial migration

Purine metabolism

Pyrimidine metabolism

N-Glycan biosynthesis

Glutathione metabolism

Histidine metabolism

Maturity onset diabetes of the young

Starch and sucrose metabolism

Proteasome

Other glycan degradation

Biotin metabolism

Glycosphingolipid biosynthesis - ganglio series

Glycosphingolipid biosynthesis - globo series

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Insulin signaling pathway

Acute myeloid leukemia

Nitrogen metabolism

Pathogenic Escherichia coli infection

beta-Alanine metabolism

Arginine and proline metabolism

Tryptophan metabolism

Galactose metabolism

Riboflavin metabolism

Tyrosine metabolism

Folate biosynthesis

Human

E. coli

Human 293

Plasma

Serum

Cell culture

Seminal Fluid

Baculovirus

HEK293

Insect

Mammalian

P.Pichia

Pancreas

Pituitary

Urine

Neomycin

Puromycin

None

1

2

false

Yes

In Stock