Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

Mutant ORF Clones

CytoSections

Mass Spec Standards

cDNA Clone Sets

Mutant CytoSections

Mutant Proteins

Over-expression Lysates

Human

BTK

RET

FGFR1

LKB1

EGFR

FGFR2

B RAF

CAMK2B

AURORA A

ABL2

CAMKK2

JNK1

NEK6

TRKB

FLT4

PRKACB

CAMK2D

MCK10

CSNK1G3

JNK2

PAK4

HER2

CAMK2G

HCK

PROTEIN KINASE D2

P38

PYK2

TNIK

TRKC

VRK2

CCK4

DYSTROPHIA MYOTONICA PROTEIN KINASE

IRAK4

AKT1

MARK2

STK25

LYK5

MARK3

FYN

CCRK

INTEGRIN LINKED KINASE

MET

PAK3

ACTIVIN RECEPTOR TYPE IA

ERBB 3

FGFR3

FGFR4

RAF1

SGK1

AMHR2

CAMKK

MELK

PKC GAMMA

ROR2

PCTAIRE1

PKC ZETA

ROR1

TRKA

ACTIVIN A RECEPTOR TYPE IC

ERK5

MYOSIN LIGHT CHAIN KINASE

TAK1

VEGF RECEPTOR 1

CHK2

DCAMKL1

GRK4

IRAK

JNK3

MAST4

MATK

DDR2

FGR

PXK

RPS6KC1

SGK3

CDK1

CDK2

CHK1

HSPC302

ALK

CGK2

FAK

MAP3K19

RYK

SYK

AKT3

BRDT

CKII ALPHA

GRK6

LIM KINASE 2

MNK1

PASK

SRC

AXL

CDK10

DYRK1A

EPH RECEPTOR A5

ERBB 4

ERK2

FES

IHC

WB

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Plasmids

Myc-DDK

Tag Free

mGFP

C-Myc/DDK

TurboGFP

C-MYC/DDK

N-His

DDK-His

C-His

C-DDK/His

C-DDK

C-Fc

N-GST and C-His

N-GST and C-HIS

His-tag

C-Fc&His

N-His-ABP (Albumin-Binding Protein)

N-GST

C-6His-Avi

C-6His

C-6×His

C- 6×His

C-Fc-Avi

C-Myc-DDK

C-hFc

C-mFc

N-10His-Flag

N-FUSION

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-XL5-DDK-His

pCMV6-XL6

pCMV6-AC

PCMV6-Neo

pCMV6-AC-Myc-DDK

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

MAPK signaling pathway

Pathways in cancer

Neurotrophin signaling pathway

ErbB signaling pathway

Focal adhesion

GnRH signaling pathway

Endocytosis

Fc epsilon RI signaling pathway

Regulation of actin cytoskeleton

Chemokine signaling pathway

Prostate cancer

Insulin signaling pathway

Progesterone-mediated oocyte maturation

T cell receptor signaling pathway

Glioma

Calcium signaling pathway

Pancreatic cancer

Colorectal cancer

Wnt signaling pathway

Axon guidance

Oocyte meiosis

Melanoma

Adherens junction

Toll-like receptor signaling pathway

B cell receptor signaling pathway

Long-term potentiation

Gap junction

Non-small cell lung cancer

Cytokine-cytokine receptor interaction

Vascular smooth muscle contraction

mTOR signaling pathway

Melanogenesis

Endometrial cancer

Chronic myeloid leukemia

Adipocytokine signaling pathway

Fc gamma R-mediated phagocytosis

VEGF signaling pathway

Renal cell carcinoma

Epithelial cell signaling in Helicobacter pylori infection

Bladder cancer

Natural killer cell mediated cytotoxicity

Acute myeloid leukemia

Tight junction

TGF-beta signaling pathway

Thyroid cancer

Long-term depression

Apoptosis

Cell cycle

Primary immunodeficiency

Olfactory transduction

NOD-like receptor signaling pathway

RIG-I-like receptor signaling pathway

Type II diabetes mellitus

Hedgehog signaling pathway

Leukocyte transendothelial migration

Prion diseases

Small cell lung cancer

p53 signaling pathway

Vibrio cholerae infection

Dorso-ventral axis formation

Jak-STAT signaling pathway

Amyotrophic lateral sclerosis (ALS)

Alzheimer's disease

Viral myocarditis

Dilated cardiomyopathy

Taste transduction

Regulation of autophagy

Cytosolic DNA-sensing pathway

Pathogenic Escherichia coli infection

Phosphatidylinositol signaling system

Hematopoietic cell lineage

PPAR signaling pathway

Purine metabolism

Circadian rhythm - mammal

Basal cell carcinoma

Ubiquitin mediated proteolysis

Hypertrophic cardiomyopathy (HCM)

Basal transcription factors

Parkinson's disease

Nucleotide excision repair

Human

E. coli

Human 293

Neomycin

None

Puromycin

1

2

Yes

In Stock