Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant Proteins

Mutant ORF Clones

Native Proteins

Human

TMPRSS4

CATHEPSIN B

CASPASE 1

PACE4

CASPASE-7

CD10

KALLIKREIN 11

ADAM15

FLIP

KALLIKREIN 6

SENP8

CALPAIN 3

DPP6

CASPASE 8

BACE1

HGF

PREPL

CATHEPSIN L

MEST

PSMA

DPP10

ECE1

PROSTATE SPECIFIC ANTIGEN

AMINOACYLASE 1

CASPASE 10

C2

EPOXIDE HYDROLASE

OTUD5

USP8

CASPASE 5

DPP3

DPP8

GGT1

MMP2

SENP7

USP20

ADAM22

PSMA4

ARTS1

CTSA

KALLIKREIN 5

MASP1

PEPD

CARBOXYPEPTIDASE M

USP16

BACE2

HAPTOGLOBIN

USP1

KALLIKREIN 15

SEC11A

USP39

ATG4B

ATG4C

CAPNS1

CASPASE 9

CD71

CPVL

KALLIKREIN 10

KALLIKREIN 12

L KYNURENINE HYDROLASE

MALT1

SIL1

TMPRSS3

TMPRSS5

USP33

ATP DEPENDENT METALLOPROTEASE YME1L1

BMP1

CASPASE 3

DPP1

MMP1

PAN2

PARK7

PSMB5

SIGNAL PEPTIDE PEPTIDASE

TRYPSIN

XPNPEP1

CARBOXYPEPTIDASE B2

GGT5

LEGUMAIN

MONOACYLGLYCEROL LIPASE

PLAT

PROTEASOME SUBUNIT ALPHA TYPE 6

PSMB8

USP14

USP44

ADAM12

AOPEP

ASRGL1

FACTOR VII

IDE

KALLIKREIN 2

UPA

USP2

USP5

XPNPEP3

C1S

DPEP1

LEUKOTRIENE A4 HYDROLASE

PP11

PROTEASOME 20S ALPHA 5

IHC

HEK293T

HEK293

E. coli

Sf9

Insect

Plasmids

Myc-DDK

mGFP

Tag Free

C-Myc/DDK

TurboGFP

C-MYC/DDK

C-His

N-His

N-GST and C-His

N-GST and C-HIS

C-6xHis

N-GST

N-His&C-His

C-DDK

C-6His

C-Fc

C-HIS

C-HUMAN

N-6His

N-Fc

N-GST, C-His

N-His-PDCD1(Pro21-Val170)

N-His/DDK

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Alzheimer's disease

Complement and coagulation cascades

Proteasome

Lysosome

Metabolic pathways

Apoptosis

Pathways in cancer

Renin-angiotensin system

Parkinson's disease

Antigen processing and presentation

NOD-like receptor signaling pathway

Huntington's disease

RIG-I-like receptor signaling pathway

Amyotrophic lateral sclerosis (ALS)

Viral myocarditis

p53 signaling pathway

Glutathione metabolism

Hematopoietic cell lineage

Arachidonic acid metabolism

Systemic lupus erythematosus

Neuroactive ligand-receptor interaction

Bladder cancer

Regulation of autophagy

Cyanoamino acid metabolism

Selenoamino acid metabolism

Taurine and hypotaurine metabolism

Prostate cancer

Endocytosis

Focal adhesion

Cytosolic DNA-sensing pathway

Colorectal cancer

Arginine and proline metabolism

GnRH signaling pathway

Leukocyte transendothelial migration

Alanine

aspartate and glutamate metabolism

Toll-like receptor signaling pathway

Wnt signaling pathway

Cytokine-cytokine receptor interaction

Melanoma

Protein export

Renal cell carcinoma

Epithelial cell signaling in Helicobacter pylori infection

Notch signaling pathway

Natural killer cell mediated cytotoxicity

Metabolism of xenobiotics by cytochrome P450

Amino sugar and nucleotide sugar metabolism

Type I diabetes mellitus

Spliceosome

B cell receptor signaling pathway

Endometrial cancer

Non-small cell lung cancer

Pancreatic cancer

Small cell lung cancer

T cell receptor signaling pathway

Tryptophan metabolism

VEGF signaling pathway

MAPK signaling pathway

PPAR signaling pathway

Glycerolipid metabolism

Hypertrophic cardiomyopathy (HCM)

Cardiac muscle contraction

Oxidative phosphorylation

Hedgehog signaling pathway

Histidine metabolism

Neurotrophin signaling pathway

beta-Alanine metabolism

Other glycan degradation

Purine metabolism

Regulation of actin cytoskeleton

Allograft rejection

Autoimmune thyroid disease

Graft-versus-host disease

Folate biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Basal transcription factors

Cell cycle

Oocyte meiosis

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Human

Neutrophils

Baculovirus

Human 293

Plasma

Neomycin

Puromycin

None

1

2

Yes

In Stock