Products

cDNA Clones

Proteins

Expression Plasmids

CytoSections

Recombinant Proteins

Mass Spec Standards

Mutant ORF Clones

cDNA Clone Sets

Human

MU OPIOID RECEPTOR

GPR56

CHRM2

GPR64

PTGER3

P2Y6

ANGIOTENSIN II TYPE 1 RECEPTOR

5HT4 RECEPTOR

SIGMA1 RECEPTOR

CRF1

CANNABINOID RECEPTOR I

GPR85

METABOTROPIC GLUTAMATE RECEPTOR 4

GPR1

GPR17

PAF RECEPTOR

ENDOTHELIN B RECEPTOR

P2RY5

METABOTROPIC GLUTAMATE RECEPTOR 1

CCR3

CD97

GPR126

GPR161

LGR7

CCR7

CX3CR1

HRH1

CMKLR1

TSH RECEPTOR

ALPHA 1A ADRENERGIC RECEPTOR

CALCITONIN RECEPTOR

ADENOSINE RECEPTOR A2A

DARC

FRIZZLED 6

P2Y2

RGR

G PROTEIN COUPLED RECEPTOR 30

GABA B RECEPTOR 1

P2Y12

NOCICEPTIN RECEPTOR

5HT7 RECEPTOR

CXCR2

DOPAMINE RECEPTOR D3

GPR155

HRH4

VIP RECEPTOR 1

CYSLT1

EMR2

ENDOTHELIN A RECEPTOR

GPR86

GPRC5C

LPHN2

CCR6

CCR9

DOPAMINE D2 RECEPTOR

EDG2

GHRELIN RECEPTOR

GPR105

GPR35

LGR5

NEUROKININ 1 RECEPTOR

PACAP RECEPTOR

5 HT 2A

ADENOSINE A1 RECEPTOR

ADENOSINE A3 RECEPTOR

CALCIUM SENSING RECEPTOR

CCR2

CXCR3

CXCR4

CXCR5

MC2 RECEPTOR

PARATHYROID HORMONE RECEPTOR 1

C5L2

FPRL1

G2A

GPR172B

GPR34

HTR2C

LEUKOTRIENE B4 RECEPTOR 2

METABOTROPIC GLUTAMATE RECEPTOR 8

TMEM147

TMEM171

AVPR V2

C5R1

CCR5

CCRL2

GHRHR

GNRHR

GPR116

GPR172A

GPR176

HRH2

MCH2

METABOTROPIC GLUTAMATE RECEPTOR 5

METABOTROPIC GLUTAMATE RECEPTOR 7

P2Y10

PROSTAGLANDIN F2 ALPHA RECEPTOR

THROMBOXANE A2 RECEPTOR

5HT5A RECEPTOR

APJ RECEPTOR

IHC

HEK293T

HEK293

E. coli

Plasmids

Myc-DDK

mGFP

Tag Free

TurboGFP

C-MYC/DDK

C-Myc/DDK

C-His

N-His

N-His-ABP (Albumin-Binding Protein)

C-Fc

C-HUMAN

N-GST and C-His

N-TRX

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL4

pCMV6-XL5

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-AC-Myc-DDK

No

Yes

LentiORF

TrueORF

TrueClone

TrueORF Gold, TrueORF

MVPro

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Neuroactive ligand-receptor interaction

Calcium signaling pathway

Chemokine signaling pathway

Cytokine-cytokine receptor interaction

Vascular smooth muscle contraction

Gap junction

Melanogenesis

Regulation of actin cytoskeleton

Endocytosis

Basal cell carcinoma

Colorectal cancer

Pathways in cancer

Wnt signaling pathway

Long-term depression

Olfactory transduction

Renin-angiotensin system

Complement and coagulation cascades

Long-term potentiation

Taste transduction

Epithelial cell signaling in Helicobacter pylori infection

Autoimmune thyroid disease

Axon guidance

Leukocyte transendothelial migration

GnRH signaling pathway

Huntington's disease

Parkinson's disease

Dilated cardiomyopathy

Human

Neomycin

Puromycin

None

1

2

Yes

In Stock