Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Native Proteins

Human

Mouse

Humon

BDNF

ALPHA 1 ANTITRYPSIN

EGFR

BETA CATENIN

HNF 4 ALPHA

CD44

TCF4

JNK1

PAX3

CD133

IGF1

JNK2

ICAM2

M-CSF

C-MYB

CD32B

ER81

CD147

G CSF

SDF1

CD16

LDL RECEPTOR

AKT1

PTCH1

PRION PROTEIN PRP

HGF

LAMP2

STAT6

ACTIVIN RECEPTOR TYPE IA

CD14

CD95

THYROID PEROXIDASE

IGF2

INTEGRIN BETA 1

MAP2

SMAD2

CD105

FIBRONECTIN

NCR3

SMAD3

CD4

GATA2

TCF7

CD56

FLT3 LIGAND

IL6R

VCAM1

AKT3

CCN4

CD244

CKII ALPHA

EGF

GCSF RECEPTOR

PVRL1

RUNX1

SRC

CD8A

CD97

LEF1

AXIN 1

CHRDL1

GM CSF

KLF6

L1CAM

TAZ

TCF3 / E2A

BCL2

CASEIN KINASE 1 ALPHA

CD16B

CD71

ERK1

SMAD5

ALPHA ACTININ

APH1A

CASPASE 3

CD30

CYSTATIN C

E CADHERIN

GATA3

GFAP

JMJD6

PAX7

TAL1

CD32A

CD34

CD51

GLI1

INSULIN

MANNOSE PHOSPHATE ISOMERASE

CD117

GAPDH

GDF 9

LIF

SMAD1

SONIC HEDGEHOG

UPA

BCL X

DDX3

KLF4

STAT5A

IHC

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Pichia

Plasmids

Myc-DDK

mGFP

C-Myc/DDK

Tag Free

TurboGFP

C-MYC/DDK

C-His

N-His

C-Fc

C-DDK

C-6His

DDK-His

N-GST and C-His

C-DDK/His

N-GST and C-HIS

C-Fc&His

His-tag

N-Trx&His

C-6His-Avi

C-6×His

C-mFc

N-His-ABP (Albumin-Binding Protein)

C-8His

C-Avi-6His

C-Human Fc

C-hFc

N-6His

N-His-Trx

C- 6×His

C-Flag,C-6His

C-HIS

C-Human Fc and 6×His

C-Mouse Fc

C-Mouse Fc and 6×His

N-6His-Avi

N-AVI&His

N-Avi

N-FC

N-Fc

N-GST

N-His&Flag

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Pathways in cancer

Cytokine-cytokine receptor interaction

Focal adhesion

MAPK signaling pathway

Colorectal cancer

Hematopoietic cell lineage

Wnt signaling pathway

Regulation of actin cytoskeleton

Cell adhesion molecules (CAMs)

Prostate cancer

Jak-STAT signaling pathway

Melanoma

Adherens junction

Pancreatic cancer

Endometrial cancer

ErbB signaling pathway

Basal cell carcinoma

Neurotrophin signaling pathway

TGF-beta signaling pathway

Leukocyte transendothelial migration

Natural killer cell mediated cytotoxicity

Acute myeloid leukemia

Glioma

Endocytosis

Melanogenesis

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

T cell receptor signaling pathway

Progesterone-mediated oocyte maturation

Hedgehog signaling pathway

Insulin signaling pathway

Fc gamma R-mediated phagocytosis

GnRH signaling pathway

Fc epsilon RI signaling pathway

Toll-like receptor signaling pathway

Small cell lung cancer

Tight junction

Maturity onset diabetes of the young

Chronic myeloid leukemia

Non-small cell lung cancer

Epithelial cell signaling in Helicobacter pylori infection

ECM-receptor interaction

Hypertrophic cardiomyopathy (HCM)

Gap junction

Adipocytokine signaling pathway

Dilated cardiomyopathy

Metabolic pathways

B cell receptor signaling pathway

Chemokine signaling pathway

Pathogenic Escherichia coli infection

Apoptosis

Type II diabetes mellitus

Renal cell carcinoma

mTOR signaling pathway

VEGF signaling pathway

Thyroid cancer

Systemic lupus erythematosus

Huntington's disease

Bladder cancer

Calcium signaling pathway

Axon guidance

Complement and coagulation cascades

Notch signaling pathway

NOD-like receptor signaling pathway

Alzheimer's disease

RIG-I-like receptor signaling pathway

Dorso-ventral axis formation

Autoimmune thyroid disease

p53 signaling pathway

Prion diseases

Oocyte meiosis

Primary immunodeficiency

Amyotrophic lateral sclerosis (ALS)

Long-term depression

Viral myocarditis

Antigen processing and presentation

Cell cycle

Type I diabetes mellitus

Allograft rejection

Lysosome

Glycolysis / Gluconeogenesis

Graft-versus-host disease

Tyrosine metabolism

Neuroactive ligand-receptor interaction

Renin-angiotensin system

Long-term potentiation

Vascular smooth muscle contraction

Retinol metabolism

PPAR signaling pathway

Nicotinate and nicotinamide metabolism

Phosphatidylinositol signaling system

Starch and sucrose metabolism

Parkinson's disease

ABC transporters

Amino sugar and nucleotide sugar metabolism

Fructose and mannose metabolism

Regulation of autophagy

Vibrio cholerae infection

Citrate cycle (TCA cycle)

Inositol phosphate metabolism

Pyruvate metabolism

Purine metabolism

Pyrimidine metabolism

Asthma

Drug metabolism - cytochrome P450

Drug metabolism - other enzymes

Linoleic acid metabolism

Metabolism of xenobiotics by cytochrome P450

Cardiac muscle contraction

Cysteine and methionine metabolism

Glutathione metabolism

Oxidative phosphorylation

Phenylalanine

Phenylalanine metabolism

Primary bile acid biosynthesis

Ubiquinone and other terpenoid-quinone biosynthesis

Ubiquitin mediated proteolysis

tyrosine and tryptophan biosynthesis

Galactose metabolism

Human

E. coli

Human 293

Plasma

Neomycin

Puromycin

None

1

2

Yes

In Stock