Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

Over-expression Lysates

CytoSections

Mass Spec Standards

Mutant ORF Clones

Native Proteins

Human

Mouse

Humon

BDNF

ALPHA 1 ANTITRYPSIN

EGFR

BETA CATENIN

HNF 4 ALPHA

CD44

ICAM2

JNK1

TCF4

PAX3

M-CSF

IGF1

JNK2

CD32B

ER81

C-MYB

AKT1

CD133

G CSF

CD16

SDF1

CD147

LDL RECEPTOR

HGF

PTCH1

CD14

IGF2

ACTIVIN RECEPTOR TYPE IA

LAMP2

SMAD2

STAT6

MAP2

CD95

PRION PROTEIN PRP

INTEGRIN BETA 1

SMAD3

THYROID PEROXIDASE

CD105

NCR3

CD4

CD244

EGF

FIBRONECTIN

GATA2

VCAM1

AKT3

IL6R

TCF7

CCN4

FLT3 LIGAND

GCSF RECEPTOR

KLF6

LEF1

RUNX1

SRC

AXIN 1

BCL2

CD56

CD8A

CD97

ERK1

PAX7

PVRL1

CHRDL1

CKII ALPHA

GM CSF

JMJD6

SMAD5

TCF3 / E2A

CASEIN KINASE 1 ALPHA

CASPASE 3

CD71

KLF4

L1CAM

TAZ

APH1A

CD117

CD30

CD34

GATA3

ALPHA ACTININ

CYSTATIN C

E CADHERIN

SMAD1

TAL1

CD16B

CD32A

CD51

CD70

GAPDH

GFAP

GLI1

INSULIN

LIF

BCL X

CDCP1

GDF 9

MANNOSE PHOSPHATE ISOMERASE

SONIC HEDGEHOG

STAT5A

IHC

WB

HEK293T

E. coli

HEK293

Insect

Sf9

CHO

Pichia

Hi-5 insect

Plasmids

Myc-DDK

C-Myc/DDK

mGFP

Tag Free

TurboGFP

C-MYC/DDK

His-tag

C-His

N-His

C-Fc

C-6His

C-DDK

DDK-His

N-GST and C-His

C-DDK/His

N-GST and C-HIS

C-Fc&His

N-Trx&His

C-6His-Avi

C-hFc

C-mFc

N-His-ABP (Albumin-Binding Protein)

C-8His

C-Avi&His

C-Avi-6His

His-Cam-tag

N-6His

N-His-Trx

hIgG-His-tag

C-FLAG-6His

C-Flag,C-6His

C-HIS

C-Human Fc

N-6His&Avi

N-6His-Avi

N-AVI&His

N-Avi

N-FC

N-Fc

N-GST

N-His&Flag

N-His-SUMO

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

WB

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

Induced pluripotent stem cells

P450

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

ELISA.
Western Blot.

FACS

Suitable for use in ELISA.

ELISA.

ELISA.

ELISA.
Western blot.
Lateral flow.

Immunogen

Secreted

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

000 or less. This should be performed in a cold room.

1 mM DTT

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

RIA.
Western blot.

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Apo AI can be renatured into PBS

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA and Western blot.

ELISA. Can be used for all immunological/laboratory methods requiring highly purified antigens.

ELISA.
Western blot.

ELISA: 0.4-0.8 µg/well.
After thawing

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Second order rate constants for inhibition of:
uPA = 6.3 x 10e6 M-1s-1
tPA = 5.7 x 10e6 M-1s-1

Sodium citric aci d 0.8g

Specific methodologies have not been tested using this product.

Suitable for ELISA and Western blot.

Suitable for use in ELISA and Western blot.

Suitable for use in Western blot.

Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces

TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14

VICTOR X3)

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

calibrator in immunoassays and in vitro studies.

calibrator in immunoassays and in vitro studies.

completely thaw the tube at 37°C without agitation

e.g. Dulbecco's PBS).

flicking or mixing

glassware and microcarrier beads. After spin and opening of the vial

including plasticware

mix thoroughly by vortexing before use!

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

then gently mix the contents with a pipette.

Pathways in cancer

Cytokine-cytokine receptor interaction

Focal adhesion

MAPK signaling pathway

Colorectal cancer

Hematopoietic cell lineage

Wnt signaling pathway

Regulation of actin cytoskeleton

Cell adhesion molecules (CAMs)

Prostate cancer

Jak-STAT signaling pathway

Melanoma

Pancreatic cancer

Adherens junction

ErbB signaling pathway

Endometrial cancer

Basal cell carcinoma

Neurotrophin signaling pathway

TGF-beta signaling pathway

Natural killer cell mediated cytotoxicity

Leukocyte transendothelial migration

Acute myeloid leukemia

Glioma

Endocytosis

Melanogenesis

T cell receptor signaling pathway

Hedgehog signaling pathway

Progesterone-mediated oocyte maturation

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Insulin signaling pathway

Fc gamma R-mediated phagocytosis

Toll-like receptor signaling pathway

Fc epsilon RI signaling pathway

GnRH signaling pathway

Small cell lung cancer

Tight junction

Chronic myeloid leukemia

Maturity onset diabetes of the young

Non-small cell lung cancer

Epithelial cell signaling in Helicobacter pylori infection

ECM-receptor interaction

Gap junction

Adipocytokine signaling pathway

Hypertrophic cardiomyopathy (HCM)

B cell receptor signaling pathway

Metabolic pathways

Chemokine signaling pathway

Dilated cardiomyopathy

Pathogenic Escherichia coli infection

Apoptosis

Renal cell carcinoma

Type II diabetes mellitus

mTOR signaling pathway

VEGF signaling pathway

Thyroid cancer

Systemic lupus erythematosus

Huntington's disease

Bladder cancer

Calcium signaling pathway

Axon guidance

NOD-like receptor signaling pathway

Complement and coagulation cascades

Alzheimer's disease

Notch signaling pathway

RIG-I-like receptor signaling pathway

Dorso-ventral axis formation

Autoimmune thyroid disease

p53 signaling pathway

Oocyte meiosis

Prion diseases

Primary immunodeficiency

Long-term depression

Amyotrophic lateral sclerosis (ALS)

Cell cycle

Viral myocarditis

Antigen processing and presentation

Type I diabetes mellitus

Allograft rejection

Lysosome

Glycolysis / Gluconeogenesis

Graft-versus-host disease

Tyrosine metabolism

Renin-angiotensin system

Long-term potentiation

Neuroactive ligand-receptor interaction

Vascular smooth muscle contraction

Retinol metabolism

Nicotinate and nicotinamide metabolism

PPAR signaling pathway

Phosphatidylinositol signaling system

Parkinson's disease

Starch and sucrose metabolism

Amino sugar and nucleotide sugar metabolism

Fructose and mannose metabolism

Vibrio cholerae infection

Regulation of autophagy

ABC transporters

Citrate cycle (TCA cycle)

Pyruvate metabolism

Inositol phosphate metabolism

Purine metabolism

Pyrimidine metabolism

Asthma

Drug metabolism - cytochrome P450

Drug metabolism - other enzymes

Linoleic acid metabolism

Metabolism of xenobiotics by cytochrome P450

Cardiac muscle contraction

Oxidative phosphorylation

Cysteine and methionine metabolism

Phenylalanine

Phenylalanine metabolism

Primary bile acid biosynthesis

Ubiquinone and other terpenoid-quinone biosynthesis

Ubiquitin mediated proteolysis

tyrosine and tryptophan biosynthesis

Glutathione metabolism

Galactose metabolism

Human

E. coli

Human 293

Cardiac muscle

Plasma

Urine

Neomycin

Puromycin

None

1

2

Yes

In Stock