Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Over-expression Lysates

Mutant Proteins

Lentiviral Particles

Native Proteins

Mutant CytoSections

Human

Mouse

Humon

BRCA1

BTK

GLUCOKINASE

MENIN

BRCA2

MLH1

TUBERIN

MSH2

P53

RET

NOTCH3

FGFR1

NF2

CREM

PDE9A

BDNF

VEGFA

NRG1

ALPHA 1 ANTITRYPSIN

CTNND1

WILMS TUMOR PROTEIN

MSH6

CONSTITUTIVE ANDROSTANE RECEPTOR

G PROTEIN ALPHA S

DYSTROBREVIN ALPHA

MU OPIOID RECEPTOR

LKB1

EMA

ASPARTATE BETA HYDROXYLASE

EGFR

FGF1

CACNA1G

BETA CATENIN

IKAROS

HNF 4 ALPHA

ESTROGEN RELATED RECEPTOR GAMMA

FH

CD46

PI 3 KINASE CATALYTIC SUBUNIT ALPHA

TPM1

ATAXIN 3

CACNA1C

APC11

TGIF

HMGA1

TAU

TCF7L2

CD1E

FGFR2

GLUCOCORTICOID RECEPTOR

GPR56

PML PROTEIN

ALPHA DYSTROGLYCAN

MAX

B RAF

CAMK2B

GM CSF RECEPTOR ALPHA

P38

AURORA A

HFE

PRKCBP1

UBE2V1

RGS3

ABL2

AMYLOID PRECURSOR PROTEIN

MSF

PRKAR1B

CD36

CD44

P73

CAMKK2

MBD1

P16INK4A

BIM

CACNB2

CD59

GELSOLIN

MID1

OGG1

P21 RAS

PTEN

PTPN20B

SORBS1

TCF4

ENSA

JNK1

MITF

NEK6

TRKB

ALPHA SYNUCLEIN

FLT4

PRKACB

ALKALINE PHOSPHATASE

CAMK2D

DIAZEPAM BINDING INHIBITOR

MCK10

S100 CALCIUM BINDING PROTEIN A13

TMPRSS4

ACRV1

KCHIP2

IHC

WB

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Pichia

Baculovirus

Plasmids

Particles

Myc-DDK

Tag Free

mGFP

C-Myc/DDK

TurboGFP

C-MYC/DDK

C-His

N-His

C-Fc

N-GST and C-His

C-DDK

C-6His

C-DDK/His

DDK-His

N-GST and C-HIS

N-GST

His-tag

N-His-ABP (Albumin-Binding Protein)

C-Fc&His

C-6His-Avi

C-HIS

C-mFc

N-His&C-His

N-6His

C-8His

C-Human Fc

N-His-Trx

N-Trx&His

C-10His

C-6×His

C-hFc

C-Mouse Fc

C-Fc-Avi

N-GST&His

N-His&SUMO

C-Avi&6His

N-Fc

Tag-free

C-Avi-6His

C-Mouse Fc and 6×His

C- 6×His

C-6xHis

N-HIS

N-His&C-Fc

N-His&T7

N-MARI

N-T7&C-His

N-Trx

C-Fc&AVI

C-Human Fc and 6×His

C-hFc&His

N-8His

N-His/DDK

C-Fc/His

C-Flag

C-HUMAN

N-6His-Avi

N-GST, C-His

N-His&Flag

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc and C-6×His

C-6xHis/AVI

C-AVI&His

C-AVI-PLUS

C-Avi

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FC-6HIS&C-FC-Flag

C-Fc-Flag&C-Fc-6His

C-Flag,C-6His

C-GST

C-HIS/DDK

C-Myc-DDK

C-hIgG2 Fc

C-hIgG4 Fc

C-mFC

N-6His-FLAG

N-6His-Flag

N-AVI

N-AVI&His

N-Avi

N-Avi&6His

N-DDK

N-DDK/His

N-FC

N-FLAG

N-FUSION

N-Fc-FLAG

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-His-GST

N-Human Fc

N-T7

N-TRX

N-TRXA

N-hFc

N-mFc

N-terminal His

Tag free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

pCMV6-AC-Myc-DDK

PCMV6-Neo

pCMV6-XL5-DDK-His

pCMV6-AN-Myc-DDK

pRMT-tGFP, Promoter-less GFP Reporter Vector

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Metabolic pathways

Pathways in cancer

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Neuroactive ligand-receptor interaction

Focal adhesion

Regulation of actin cytoskeleton

Chemokine signaling pathway

Neurotrophin signaling pathway

Insulin signaling pathway

Calcium signaling pathway

Jak-STAT signaling pathway

Colorectal cancer

Wnt signaling pathway

Prostate cancer

T cell receptor signaling pathway

Progesterone-mediated oocyte maturation

Pancreatic cancer

Natural killer cell mediated cytotoxicity

ErbB signaling pathway

Endocytosis

Cell cycle

Ubiquitin mediated proteolysis

Apoptosis

Toll-like receptor signaling pathway

Melanoma

Axon guidance

GnRH signaling pathway

Endometrial cancer

B cell receptor signaling pathway

Chronic myeloid leukemia

Fc epsilon RI signaling pathway

Adherens junction

Hematopoietic cell lineage

Glioma

Leukocyte transendothelial migration

Oocyte meiosis

p53 signaling pathway

Purine metabolism

Renal cell carcinoma

Small cell lung cancer

Gap junction

Alzheimer's disease

Vascular smooth muscle contraction

Melanogenesis

VEGF signaling pathway

Tight junction

Non-small cell lung cancer

Cell adhesion molecules (CAMs)

Dilated cardiomyopathy

Acute myeloid leukemia

Long-term potentiation

Bladder cancer

Fc gamma R-mediated phagocytosis

Huntington's disease

Long-term depression

TGF-beta signaling pathway

Type II diabetes mellitus

Adipocytokine signaling pathway

Hypertrophic cardiomyopathy (HCM)

NOD-like receptor signaling pathway

mTOR signaling pathway

Lysosome

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Phosphatidylinositol signaling system

Thyroid cancer

Amyotrophic lateral sclerosis (ALS)

Glycolysis / Gluconeogenesis

Complement and coagulation cascades

RIG-I-like receptor signaling pathway

ECM-receptor interaction

Olfactory transduction

Epithelial cell signaling in Helicobacter pylori infection

PPAR signaling pathway

Primary immunodeficiency

Inositol phosphate metabolism

Basal cell carcinoma

Mismatch repair

Pathogenic Escherichia coli infection

Viral myocarditis

Cytosolic DNA-sensing pathway

Cardiac muscle contraction

Hedgehog signaling pathway

Amino sugar and nucleotide sugar metabolism

Antigen processing and presentation

Galactose metabolism

Drug metabolism - cytochrome P450

Starch and sucrose metabolism

Metabolism of xenobiotics by cytochrome P450

Pyrimidine metabolism

Arginine and proline metabolism

Valine

Parkinson's disease

Retinol metabolism

Homologous recombination

Maturity onset diabetes of the young

Tyrosine metabolism

Glycerophospholipid metabolism

Autoimmune thyroid disease

Glycerolipid metabolism

Notch signaling pathway

Arachidonic acid metabolism

Prion diseases

Base excision repair

Drug metabolism - other enzymes

Nucleotide excision repair

Taste transduction

leucine and isoleucine degradation

ABC transporters

Vibrio cholerae infection

Dorso-ventral axis formation

Systemic lupus erythematosus

Fructose and mannose metabolism

Type I diabetes mellitus

Fatty acid metabolism

Pyruvate metabolism

Lysine degradation

Sphingolipid metabolism

Tryptophan metabolism

Cysteine and methionine metabolism

Glutathione metabolism

Regulation of autophagy

SNARE interactions in vesicular transport

Allograft rejection

Proteasome

Histidine metabolism

Butanoate metabolism

Citrate cycle (TCA cycle)

Nitrogen metabolism

Pentose phosphate pathway

Propanoate metabolism

Alanine

aspartate and glutamate metabolism

Androgen and estrogen metabolism

Linoleic acid metabolism

Graft-versus-host disease

Renin-angiotensin system

Oxidative phosphorylation

Phenylalanine metabolism

Ether lipid metabolism

Porphyrin and chlorophyll metabolism

Aminoacyl-tRNA biosynthesis

Riboflavin metabolism

Glycosaminoglycan degradation

Spliceosome

DNA replication

beta-Alanine metabolism

Selenoamino acid metabolism

Ribosome

Other glycan degradation

Asthma

Glycine

serine and threonine metabolism

Non-homologous end-joining

Steroid biosynthesis

Circadian rhythm - mammal

Pantothenate and CoA biosynthesis

C21-Steroid hormone metabolism

Primary bile acid biosynthesis

leucine and isoleucine biosynthesis

Folate biosynthesis

Terpenoid backbone biosynthesis

N-Glycan biosynthesis

alpha-Linolenic acid metabolism

Basal transcription factors

Pentose and glucuronate interconversions

Ascorbate and aldarate metabolism

Synthesis and degradation of ketone bodies

Glyoxylate and dicarboxylate metabolism

One carbon pool by folate

Limonene and pinene degradation

Nicotinate and nicotinamide metabolism

RNA degradation

Thiamine metabolism

Glycosphingolipid biosynthesis - globo series

Ubiquinone and other terpenoid-quinone biosynthesis

Glycosphingolipid biosynthesis - ganglio series

Caffeine metabolism

Protein export

Biosynthesis of unsaturated fatty acids

Glycosphingolipid biosynthesis - lacto and neolacto series

Methane metabolism

Taurine and hypotaurine metabolism

Sulfur metabolism

Phenylalanine

tyrosine and tryptophan biosynthesis

Fatty acid biosynthesis

Fatty acid elongation in mitochondria

Heparan sulfate biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

O-Glycan biosynthesis

RNA polymerase

Vitamin B6 metabolism

Biotin metabolism

Chondroitin sulfate biosynthesis

D-Arginine and D-ornithine metabolism

D-Glutamine and D-glutamate metabolism

Keratan sulfate biosynthesis

Lysine biosynthesis

Human

E. coli

Human 293

Plasma

Baculovirus

CHO Stable

HEK293

Leukocytes

Mammalian

Neutrophils

P.Pichia

Pancreas

Pituitary

Neomycin

Puromycin

None

1

2

Yes

In Stock