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Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant B, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Products
Purified recombinant protein of Human receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIPK1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Recombinant protein of human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3, full length, with C-terminal HIS tag, expressed in E.Coli, 50 ug
| Tag | C-HIS |
| Expression Host | E. coli |
Purified recombinant protein of Human gasdermin D (GSDMD), transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human Sp1 transcription factor (SP1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human forkhead box O4 (FOXO4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human prohibitin 2 (PHB2), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant A, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human androgen receptor (AR), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human wingless-type MMTV integration site family, member 4 (WNT4), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human SMAD family member 1 (SMAD1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
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Target Symbol
- TNF ALPHA (15)
- IGF1 (14)
- PTPRN (12)
- VEGFA (12)
- FGF1 (10)
- IL1 BETA (10)
- NFYA (10)
- NRG1 (10)
- BDNF (9)
- EOTAXIN 3 (9)
- HMG1 (9)
- IL4 (9)
- IL6 (9)
- SDF1 (9)
- ALPHA SYNUCLEIN (8)
- BMP2 (8)
- FSTL1 (8)
- GABARAP (8)
- HIP2 (8)
- IL33 (8)
- IL8 (8)
- INTERFERON ALPHA 2 (8)
- PDGF AA (8)
- SONIC HEDGEHOG (8)
- SUMO 3 (8)
- BNP (7)
- CAMK2B (7)
- FABP4 (7)
- FERRITIN HEAVY CHAIN (7)
- IL3 (7)
- TRAIL (7)
- BLNK (6)
- DESMIN (6)
- GDNF (6)
- GLUTAMINE SYNTHETASE (6)
- IL2 (6)
- MAP1LC3A (6)
- NEUROTROPHIN 3 (6)
- NHP2 (6)
- TGIF (6)
- TRIM72 (6)
- AMPHIREGULIN (5)
- ANNEXIN VI (5)
- APE1 (5)
- APOLIPOPROTEIN E (5)
- ASB13 (5)
- ASF1A (5)
- BETA ARRESTIN 1 (5)
- BID (5)
- BIRC5 (5)
- CALCITONIN (5)
- CASPASE 10 (5)
- CSNK1G2 (5)
- CSPS (5)
- CXCL14 (5)
- EGF (5)
- EIF1A (5)
- EIF4EBP1 (5)
- FGF2 (5)
- FGF21 (5)
- FIBRONECTIN (5)
- GABARAPL1 (5)
- GADD45B (5)
- GLUTATHIONE TRANSFERASE ZETA 1 (5)
- HAO1 (5)
- IL15 (5)
- IL17 (5)
- IL17F (5)
- IL1RA (5)
- IL7 (5)
- INHIBIN BETA C CHAIN (5)
- INTERFERON GAMMA (5)
- KGF (5)
- KLF6 (5)
- LACTB2 (5)
- LAP2 (5)
- LEPTIN (5)
- LIF (5)
- LSM4 (5)
- LYSOPHOSPHOLIPASE 1 (5)
- MAX (5)
- MGMT (5)
- MYOZENIN 2 (5)
- NCK (5)
- NEDD8 (5)
- NEURITIN (5)
- NGAL (5)
- OGG1 (5)
- ONCOSTATIN M (5)
- PARATHYROID HORMONE (5)
- PDCD10 (5)
- PDGF BETA (5)
- PDGFA (5)
- PEROXIREDOXIN 4 (5)
- PGP9.5 (5)
- PIN4 (5)
- PKIB (5)
- SENP2 (5)
- SENP7 (5)
- SUMO 1 (5)
Tags
- N-His (2709)
- Tag Free (580)
- C-His (464)
- N-GST and C-His (435)
- N-GST and C-HIS (150)
- N-GST (105)
- N-His-ABP (Albumin-Binding Protein) (52)
- His-tag (46)
- N-His&C-His (40)
- N-T7&C-His (24)
- N-Trx&His (24)
- N-His-Trx (18)
- N-GST, C-His (13)
- N-GST&His (8)
- N-His&SUMO (8)
- Tag-free (7)
- N-6His (6)
- N-His PDCD1(Pro21-Val170) (5)
- N-HIS (4)
- N-His&C-Fc (4)
- N-His&T7 (4)
- N-His-GST (4)
- N-MARI (4)
- N-Trx (4)
- C-Avi&6His (3)
- C-HIS (3)
- N-Avi (3)
- Myc-DDK (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- C-HIS/C (1)
- N-AVI (1)
- N-DDK (1)
- N-FUSION (1)
- N-GFP (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
Families
- Druggable Genome (2387)
- Secreted Protein (778)
- Transmembrane (672)
- Transcription Factors (663)
- ES Cell Differentiation/IPS (259)
- Stem cell - Pluripotency (171)
- Protein Kinase (163)
- Protease (137)
- Adult stem cells (87)
- Embryonic stem cells (75)
- Phosphatase (63)
- Induced pluripotent stem cells (41)
- Ion Channels: Other (40)
- Cancer stem cells (31)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (26)
- Stem cell relevant signaling - JAK/STAT signaling pathway (25)
- Stem cell relevant signaling - DSL/Notch pathway (15)
- Stem cell relevant signaling - Wnt Signaling pathway (15)
- 10 mM Tris-HCl (12)
- GPCR (12)
- Nuclear Hormone Receptor (12)
- the solution can be further dialyzed against PBS (phosphate buffered saline (12)
- 2 mM dithiothreitol (10)
- e.g. Dulbecco's PBS). (10)
-
ELISA.
Western Blot. (9) - Ion Channels: Potassium (8)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (8)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (6) - P450 (6)
- pH 7.4). For immunization purposes (6)
- Ion Channels: Transient receptor potential (4)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) -
pH 7.4).
For immunization purposes (4) -
pH 7.4).
For immunization purposes (4) - Ion Channels: Cys-loop Receptors (3)
- Western Blot. (3)
- 0.1% (w/v) insulin (2)
- 10mM Tris-HCl (2)
- 1mM DTT (2)
- 2 mM dithiothreitol (2)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 2mM dithiothreitol (2)
- 63mM sodium phosphate (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - Can be used as standard in a Sandwich ELISA. (2)
- Ion Channels: Sodium (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) - Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues (2)
-
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
- e.g.Dulbecco's PBS). (2)
- including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue. (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
-
ELISA.
Western blotting. (1) -
Positive control for Western blot analysis.
Standard for ELISA. (1) -
Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Ion Channels: ATP Receptors (1)
- Marx UC (1)
-
Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
-
Western Blot.
Protocol: 1. Lauber T (1) - Western Blotting. (1)
Pathways
- Metabolic pathways (378)
- Cytokine-cytokine receptor interaction (349)
- Pathways in cancer (305)
- MAPK signaling pathway (228)
- Jak-STAT signaling pathway (155)
- Chemokine signaling pathway (132)
- Hematopoietic cell lineage (122)
- Focal adhesion (119)
- Regulation of actin cytoskeleton (118)
- Melanoma (110)
- Apoptosis (99)
- Toll-like receptor signaling pathway (99)
- T cell receptor signaling pathway (98)
- Natural killer cell mediated cytotoxicity (95)
- NOD-like receptor signaling pathway (86)
- Alzheimer's disease (84)
- Ubiquitin mediated proteolysis (81)
- Prostate cancer (78)
- Systemic lupus erythematosus (76)
- ErbB signaling pathway (71)
- Fc epsilon RI signaling pathway (70)
- Cell cycle (69)
- TGF-beta signaling pathway (69)
- Type I diabetes mellitus (69)
- Hypertrophic cardiomyopathy (HCM) (68)
- Neurotrophin signaling pathway (68)
- Cytosolic DNA-sensing pathway (65)
- Glioma (64)
- Wnt signaling pathway (64)
- Graft-versus-host disease (62)
- p53 signaling pathway (62)
- Huntington's disease (61)
- Oocyte meiosis (61)
- Parkinson's disease (61)
- Dilated cardiomyopathy (59)
- RIG-I-like receptor signaling pathway (59)
- Amyotrophic lateral sclerosis (ALS) (55)
- Prion diseases (51)
- Renal cell carcinoma (51)
- Acute myeloid leukemia (50)
- Asthma (50)
- Insulin signaling pathway (50)
- Hedgehog signaling pathway (49)
- Melanogenesis (49)
- Pancreatic cancer (49)
- Pyrimidine metabolism (49)
- Allograft rejection (48)
- PPAR signaling pathway (48)
- mTOR signaling pathway (48)
- Adipocytokine signaling pathway (47)
- Small cell lung cancer (46)
- Colorectal cancer (44)
- Gap junction (44)
- Chronic myeloid leukemia (43)
- B cell receptor signaling pathway (42)
- Endocytosis (42)
- Purine metabolism (42)
- Regulation of autophagy (42)
- Bladder cancer (41)
- Nitrogen metabolism (41)
- Basal cell carcinoma (40)
- Glycolysis / Gluconeogenesis (40)
- Progesterone-mediated oocyte maturation (40)
- Base excision repair (39)
- Arginine and proline metabolism (38)
- Ribosome (38)
- Autoimmune thyroid disease (37)
- Antigen processing and presentation (36)
- Calcium signaling pathway (35)
- Leukocyte transendothelial migration (35)
- Type II diabetes mellitus (35)
- Spliceosome (34)
- Axon guidance (33)
- GnRH signaling pathway (32)
- Long-term potentiation (32)
- VEGF signaling pathway (32)
- Epithelial cell signaling in Helicobacter pylori infection (30)
- Adherens junction (29)
- Vascular smooth muscle contraction (29)
- Endometrial cancer (28)
- Fructose and mannose metabolism (28)
- Cell adhesion molecules (CAMs) (26)
- Long-term depression (26)
- Tyrosine metabolism (26)
- Amino sugar and nucleotide sugar metabolism (25)
- Neuroactive ligand-receptor interaction (25)
- Oxidative phosphorylation (25)
- Primary immunodeficiency (24)
- Complement and coagulation cascades (23)
- Drug metabolism - other enzymes (23)
- Glutathione metabolism (23)
- Phenylalanine metabolism (23)
- Porphyrin and chlorophyll metabolism (23)
- ECM-receptor interaction (22)
- Glycine (22)
- Non-small cell lung cancer (22)
- serine and threonine metabolism (22)
- Cysteine and methionine metabolism (21)
- Metabolism of xenobiotics by cytochrome P450 (21)
- Tryptophan metabolism (21)
- Fc gamma R-mediated phagocytosis (20)
- SNARE interactions in vesicular transport (20)
- Glycerophospholipid metabolism (19)
- Sulfur metabolism (19)
- Alanine (18)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (18)
- Cardiac muscle contraction (18)
- Pyruvate metabolism (18)
- RNA degradation (18)
- Viral myocarditis (18)
- aspartate and glutamate metabolism (18)
- Pathogenic Escherichia coli infection (17)
- Inositol phosphate metabolism (15)
- Mismatch repair (15)
- Nucleotide excision repair (15)
- Olfactory transduction (15)
- Thyroid cancer (15)
- Tight junction (15)
- ABC transporters (14)
- Terpenoid backbone biosynthesis (14)
- Glyoxylate and dicarboxylate metabolism (13)
- Homologous recombination (13)
- Pentose phosphate pathway (13)
- Phosphatidylinositol signaling system (13)
- Propanoate metabolism (13)
- Retinol metabolism (13)
- Aminoacyl-tRNA biosynthesis (12)
- Androgen and estrogen metabolism (12)
- Citrate cycle (TCA cycle) (12)
- DNA replication (12)
- Dorso-ventral axis formation (12)
- Drug metabolism - cytochrome P450 (12)
- Arachidonic acid metabolism (11)
- Basal transcription factors (11)
- Butanoate metabolism (11)
- Fatty acid metabolism (11)
- Notch signaling pathway (11)
- Pantothenate and CoA biosynthesis (11)
- Valine (10)
- beta-Alanine metabolism (10)
- Ether lipid metabolism (9)
- Galactose metabolism (9)
- Lysine degradation (9)
- Lysosome (9)
- Maturity onset diabetes of the young (9)
- Methane metabolism (9)
- Nicotinate and nicotinamide metabolism (9)
- Pentose and glucuronate interconversions (9)
- Proteasome (9)
- Synthesis and degradation of ketone bodies (9)
- leucine and isoleucine degradation (9)
- One carbon pool by folate (8)
- Renin-angiotensin system (8)
- Selenoamino acid metabolism (8)
- Ubiquinone and other terpenoid-quinone biosynthesis (8)
- N-Glycan biosynthesis (7)
- Riboflavin metabolism (7)
- Phenylalanine (6)
- Steroid biosynthesis (6)
- Vibrio cholerae infection (6)
- tyrosine and tryptophan biosynthesis (6)
- C21-Steroid hormone metabolism (5)
- Glycosphingolipid biosynthesis - lacto and neolacto series (5)
- Heparan sulfate biosynthesis (5)
- Primary bile acid biosynthesis (5)
- Sphingolipid metabolism (5)
- Chondroitin sulfate biosynthesis (4)
- D-Arginine and D-ornithine metabolism (4)
- Histidine metabolism (4)
- Keratan sulfate biosynthesis (4)
- Linoleic acid metabolism (4)
- Non-homologous end-joining (4)
- O-Glycan biosynthesis (4)
- Starch and sucrose metabolism (4)
- Taste transduction (4)
- Taurine and hypotaurine metabolism (4)
- alpha-Linolenic acid metabolism (4)
- Circadian rhythm - mammal (3)
- Folate biosynthesis (3)
- RNA polymerase (3)
- Caffeine metabolism (2)
- D-Glutamine and D-glutamate metabolism (2)
- Glycosaminoglycan degradation (2)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (2)
- Limonene and pinene degradation (2)
- Other glycan degradation (2)
- leucine and isoleucine biosynthesis (2)
- Ascorbate and aldarate metabolism (1)
- Biosynthesis of unsaturated fatty acids (1)
- Cyanoamino acid metabolism (1)
- Fatty acid elongation in mitochondria (1)
- Glycerolipid metabolism (1)
- Glycosphingolipid biosynthesis - ganglio series (1)
- Lipoic acid metabolism (1)
- Protein export (1)
