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Target Symbol
- SNCA (22)
- VEGFA (20)
- IGF1 (16)
- TNF ALPHA (16)
- IL6 (14)
- IL4 (12)
- PTPRN (12)
- BDNF (11)
- IL2 (11)
- SDF1 (11)
- BMP2 (10)
- FGF1 (10)
- FSTL1 (10)
- GABARAP (10)
- IL1 BETA (10)
- MIF (10)
- NFYA (10)
- NRG1 (10)
- EOTAXIN 3 (9)
- HMG1 (9)
- IL3 (9)
- IL33 (9)
- IL8 (9)
- INTERFERON ALPHA 2 (9)
- RNA5-8SN2 (9)
- ALPHA SYNUCLEIN (8)
- BLNK (8)
- CA8 (8)
- CNTF (8)
- EGF (8)
- HIP2 (8)
- HSPA1A (8)
- IL16 (8)
- IL17F (8)
- IL1F10 (8)
- MAP1LC3A (8)
- MAPT (8)
- NHP2 (8)
- PDGF AA (8)
- SONIC HEDGEHOG (8)
- SUMO 3 (8)
- ASB13 (7)
- ASF1A (7)
- BID (7)
- BIRC5 (7)
- BNP (7)
- CAMK2B (7)
- CCL27 (7)
- CCL28 (7)
- EIF4EBP1 (7)
- FABP4 (7)
- FBP1 (7)
- FERRITIN HEAVY CHAIN (7)
- GABARAPL1 (7)
- GADD45B (7)
- HAO1 (7)
- HSPA5 (7)
- HSPD1 (7)
- IL17 (7)
- KLF6 (7)
- LSM4 (7)
- MAX (7)
- MGMT (7)
- NEDD8 (7)
- OGG1 (7)
- PIN4 (7)
- PKIB (7)
- S100P (7)
- TXN (7)
- UBE2G2 (7)
- YY1 (7)
- ACADM (6)
- ADPRH (6)
- AKR1C4 (6)
- ALDH1A1 (6)
- ASS1 (6)
- ATF1 (6)
- ATG5 (6)
- BCL2L2 (6)
- BDH1 (6)
- BDH2 (6)
- BMP4 (6)
- BPGM (6)
- CA13 (6)
- CCL3 (6)
- CDK2 (6)
- CLIC1 (6)
- CLIC2 (6)
- CLIC4 (6)
- CUTA (6)
- DCK (6)
- DCTN2 (6)
- DCUN1D1 (6)
- DCXR (6)
- DESMIN (6)
- DHPS (6)
- DUT (6)
- DYNLL1 (6)
- EDF1 (6)
- EIF1B (6)
Tags
- His-tag (5288)
- N-His (2709)
- Tag Free (645)
- C-His (464)
- N-GST and C-His (435)
- N-GST and C-HIS (150)
- N-GST (104)
- N-His-ABP (Albumin-Binding Protein) (52)
- N-His&C-His (40)
- T7-tag (30)
- N-T7&C-His (24)
- N-Trx&His (24)
- N-His-Trx (18)
- N-GST, C-His (13)
- N-6His (12)
- N-GST&His (8)
- N-His&SUMO (8)
- C-6His (7)
- Tag-free (7)
- N-His PDCD1(Pro21-Val170) (5)
- His-Cam-tag (4)
- N-HIS (4)
- N-His&C-Fc (4)
- N-His&T7 (4)
- N-His-GST (4)
- N-MARI (4)
- N-Trx (4)
- C-Avi&6His (3)
- C-HIS (3)
- N-Avi (3)
- CaM-tag (2)
- Calmodulin-tag (2)
- His-MBP-tag (2)
- His-Tag (2)
- Myc-DDK (2)
- N-GFP (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-TrxA (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- TrxA (2)
- C-HIS/C (1)
- N-AVI (1)
- N-DDK (1)
- N-FUSION (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
Families
- Druggable Genome (5224)
- Secreted Protein (1466)
- Transmembrane (1408)
- Transcription Factors (1178)
- ES Cell Differentiation/IPS (448)
- Stem cell - Pluripotency (376)
- Protease (340)
- Protein Kinase (288)
- Phosphatase (145)
- Adult stem cells (135)
- Embryonic stem cells (120)
- Induced pluripotent stem cells (76)
- Cancer stem cells (67)
- Ion Channels: Other (62)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (46)
- Stem cell relevant signaling - JAK/STAT signaling pathway (33)
- Nuclear Hormone Receptor (23)
- Stem cell relevant signaling - Wnt Signaling pathway (23)
- Stem cell relevant signaling - DSL/Notch pathway (17)
- GPCR (12)
- Ion Channels: Potassium (8)
- P450 (8)
- Ion Channels: Cys-loop Receptors (7)
- Ion Channels: Sodium (4)
- Ion Channels: Transient receptor potential (4)
-
ELISA.
Western Blot. (3) - ELISA. (2)
-
ELISA.
Western blot.
Lateral flow. (2) -
0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes. (1) -
0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug (1) - 0.1 mg/ml Ovalbumin (1)
- 0.1% (w/v) insulin (1)
- 0.12 mM beta-nicotinamide adenine dinucleotide (1)
- 0.5 mM EDTA (1)
-
0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes. (1) - 0.9 mM Xanthine (1)
- 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl (1)
- 1 mM DTT (1)
- 10 mM Tris-HCl (1)
-
10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug (1) - 1mM DTT (1)
- 1mM MnCl2 (1)
- 2 mM dithiothreitol (1)
- 2.3 mM Pyruvate (1)
- 2mM DTT (1)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (1) -
3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm. (1) - 5.5 mM Ethylendiaminetetraacetic acid (1)
- 50mM NaCl (1)
- 63mM sodium phosphate (1)
-
655077)
- Fluorescent plate reader (PerkinElmer (1) - 66 mM Cytochrom-C (1)
-
ELISA (0.5 µg/ml).
Western blot. (1) -
ELISA.
Western Blot.
Lateral Flow. (1) -
ELISA.
Western Blot. (1) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) -
Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard. (1) -
BML-SE211)
- 96 Well Polystyrene Microplate (1) - Cat.No (1)
- Citric acid 0.055 g (1)
-
D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination. (1) -
ELISA.
Western blot. (1) - FACS (1)
- Ion Channels: ATP Receptors (1)
- Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng (1)
-
Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate (1) -
Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container (1) -
Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5) (1) -
Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl (1) -
Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g (1) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (1) - Sodium citric aci d 0.8g (1)
- VICTOR X3) (1)
- adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate (1)
-
and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C. (1) - black (greiner bio-one (1)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1)
- e.g. Dulbecco's PBS). (1)
-
pH 7.4).
For immunization purposes (1) - pH 8.0 (1)
-
pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo (1) - reduced form (1)
- the solution can be further dialyzed against PBS (phosphate buffered saline (1)
Pathways
- Metabolic pathways (1035)
- Cytokine-cytokine receptor interaction (612)
- Pathways in cancer (507)
- MAPK signaling pathway (405)
- Chemokine signaling pathway (295)
- Regulation of actin cytoskeleton (245)
- Jak-STAT signaling pathway (244)
- Focal adhesion (231)
- Natural killer cell mediated cytotoxicity (209)
- Alzheimer's disease (208)
- T cell receptor signaling pathway (207)
- Hematopoietic cell lineage (193)
- Toll-like receptor signaling pathway (182)
- Purine metabolism (170)
- Huntington's disease (169)
- Ubiquitin mediated proteolysis (168)
- Neurotrophin signaling pathway (164)
- Parkinson's disease (159)
- Melanoma (156)
- Apoptosis (155)
- Pyrimidine metabolism (155)
- NOD-like receptor signaling pathway (149)
- Cell cycle (143)
- Prostate cancer (141)
- Wnt signaling pathway (136)
- Insulin signaling pathway (135)
- Endocytosis (134)
- ErbB signaling pathway (134)
- Fc epsilon RI signaling pathway (132)
- Renal cell carcinoma (129)
- TGF-beta signaling pathway (127)
- Antigen processing and presentation (126)
- Oocyte meiosis (123)
- Glycolysis / Gluconeogenesis (119)
- Type I diabetes mellitus (119)
- p53 signaling pathway (118)
- Spliceosome (117)
- Axon guidance (114)
- Hypertrophic cardiomyopathy (HCM) (112)
- Systemic lupus erythematosus (111)
- RIG-I-like receptor signaling pathway (109)
- Graft-versus-host disease (108)
- Amyotrophic lateral sclerosis (ALS) (107)
- Leukocyte transendothelial migration (107)
- Glioma (106)
- Cytosolic DNA-sensing pathway (105)
- Pancreatic cancer (101)
- Progesterone-mediated oocyte maturation (101)
- Arginine and proline metabolism (98)
- Ribosome (98)
- Colorectal cancer (95)
- Prion diseases (94)
- B cell receptor signaling pathway (92)
- Dilated cardiomyopathy (92)
- Melanogenesis (92)
- Adipocytokine signaling pathway (91)
- mTOR signaling pathway (88)
- Gap junction (87)
- PPAR signaling pathway (86)
- VEGF signaling pathway (86)
- Allograft rejection (85)
- Chronic myeloid leukemia (85)
- Hedgehog signaling pathway (85)
- Bladder cancer (83)
- Acute myeloid leukemia (82)
- Cell adhesion molecules (CAMs) (82)
- GnRH signaling pathway (82)
- Amino sugar and nucleotide sugar metabolism (81)
- Glutathione metabolism (81)
- Tight junction (81)
- Asthma (79)
- Autoimmune thyroid disease (77)
- Epithelial cell signaling in Helicobacter pylori infection (77)
- Oxidative phosphorylation (77)
- Long-term potentiation (76)
- Fructose and mannose metabolism (75)
- Regulation of autophagy (74)
- Small cell lung cancer (74)
- Adherens junction (71)
- Fc gamma R-mediated phagocytosis (70)
- Base excision repair (69)
- Metabolism of xenobiotics by cytochrome P450 (69)
- Proteasome (66)
- SNARE interactions in vesicular transport (66)
- Tyrosine metabolism (66)
- Nitrogen metabolism (65)
- RNA degradation (65)
- Vascular smooth muscle contraction (65)
- Type II diabetes mellitus (64)
- Calcium signaling pathway (62)
- Complement and coagulation cascades (61)
- Cysteine and methionine metabolism (61)
- Valine (58)
- Viral myocarditis (58)
- leucine and isoleucine degradation (57)
- Basal cell carcinoma (56)
- Long-term depression (56)
- Pentose phosphate pathway (56)
- Drug metabolism - cytochrome P450 (54)
- Drug metabolism - other enzymes (53)
- Endometrial cancer (53)
- Glycine (52)
- Non-small cell lung cancer (52)
- serine and threonine metabolism (52)
- Fatty acid metabolism (51)
- Cardiac muscle contraction (50)
- Pyruvate metabolism (50)
- Tryptophan metabolism (49)
- Primary immunodeficiency (48)
- Lysosome (47)
- Phenylalanine metabolism (47)
- Porphyrin and chlorophyll metabolism (47)
- Alanine (46)
- aspartate and glutamate metabolism (46)
- Pathogenic Escherichia coli infection (45)
- Butanoate metabolism (39)
- Neuroactive ligand-receptor interaction (39)
- Thyroid cancer (39)
- Glycerophospholipid metabolism (37)
- Arachidonic acid metabolism (35)
- Aminoacyl-tRNA biosynthesis (34)
- ECM-receptor interaction (34)
- Propanoate metabolism (33)
- RNA polymerase (33)
- Terpenoid backbone biosynthesis (32)
- Galactose metabolism (31)
- Homologous recombination (31)
- Nucleotide excision repair (31)
- Retinol metabolism (31)
- Sulfur metabolism (31)
- Androgen and estrogen metabolism (30)
- Citrate cycle (TCA cycle) (30)
- Inositol phosphate metabolism (29)
- Starch and sucrose metabolism (28)
- Glyoxylate and dicarboxylate metabolism (27)
- Nicotinate and nicotinamide metabolism (27)
- Phosphatidylinositol signaling system (27)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (26)
- DNA replication (26)
- Dorso-ventral axis formation (26)
- beta-Alanine metabolism (26)
- Lysine degradation (25)
- Basal transcription factors (23)
- Mismatch repair (21)
- Olfactory transduction (21)
- Pentose and glucuronate interconversions (21)
- Synthesis and degradation of ketone bodies (21)
- Histidine metabolism (20)
- Selenoamino acid metabolism (20)
- Vibrio cholerae infection (20)
- Pantothenate and CoA biosynthesis (19)
- Riboflavin metabolism (19)
- One carbon pool by folate (18)
- Maturity onset diabetes of the young (17)
- Methane metabolism (17)
- Ether lipid metabolism (15)
- ABC transporters (14)
- Folate biosynthesis (13)
- N-Glycan biosynthesis (13)
- Notch signaling pathway (13)
- Sphingolipid metabolism (13)
- Limonene and pinene degradation (12)
- C21-Steroid hormone metabolism (11)
- Fatty acid elongation in mitochondria (11)
- Glycerolipid metabolism (11)
- Glycosphingolipid biosynthesis - lacto and neolacto series (11)
- Primary bile acid biosynthesis (11)
- Non-homologous end-joining (10)
- Other glycan degradation (10)
- Phenylalanine (10)
- Steroid biosynthesis (10)
- Taste transduction (10)
- Taurine and hypotaurine metabolism (10)
- Ubiquinone and other terpenoid-quinone biosynthesis (10)
- tyrosine and tryptophan biosynthesis (10)
- Biosynthesis of unsaturated fatty acids (9)
- Heparan sulfate biosynthesis (9)
- D-Arginine and D-ornithine metabolism (8)
- Linoleic acid metabolism (8)
- Renin-angiotensin system (8)
- Vitamin B6 metabolism (8)
- Ascorbate and aldarate metabolism (7)
- Chondroitin sulfate biosynthesis (6)
- Glycosaminoglycan degradation (6)
- O-Glycan biosynthesis (6)
- Thiamine metabolism (6)
- leucine and isoleucine biosynthesis (6)
- Circadian rhythm - mammal (5)
- Cyanoamino acid metabolism (5)
- Glycosphingolipid biosynthesis - ganglio series (5)
- Protein export (5)
- Caffeine metabolism (4)
- D-Glutamine and D-glutamate metabolism (4)
- Keratan sulfate biosynthesis (4)
- alpha-Linolenic acid metabolism (4)
- Fatty acid biosynthesis (2)
- Glycosphingolipid biosynthesis - globo series (2)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (2)
- Lysine biosynthesis (2)
- Lipoic acid metabolism (1)