Products

Proteins

Recombinant Proteins

Human

ACID PHOSPHATASE

CALCINEURIN B

PHOSPHOSERINE PHOSPHATASE

PP1C GAMMA

PPM1A

PPM1G

PTPN11

SHP1

PPP1A

DUSP10

PLEIOTROPHIN

PRY

CALCINEURIN A

CD45

CTDSPL

DUSP11

DUSP22

DUSP26

DUSP5

PP2A-BETA

PP4R4

PPM1M

PPP1R10

PPP2R3B

PPP2R5D

PPP4C

PPTC7

PROSTATIC ACID PHOSPHATASE

PTEN

PTP1B

PTP4A3

PTPRC

SHP2

SIRP ALPHA

HEK293T

HEK293

E. coli

Sf9

N-His

C-His

N-GST and C-His

N-His&C-His

N-HIS

N-His-ABP (Albumin-Binding Protein)

Tag Free

Druggable Genome

Secreted Protein

Transmembrane

Transcription Factors

ES Cell Differentiation/IPS

Stem cell - Pluripotency

Protein Kinase

Protease

Adult stem cells

Embryonic stem cells

Phosphatase

Induced pluripotent stem cells

Ion Channels: Other

Cancer stem cells

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

10 mM Tris-HCl

GPCR

Nuclear Hormone Receptor

the solution can be further dialyzed against PBS (phosphate buffered saline

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Ion Channels: Potassium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

P450

pH 7.4). For immunization purposes

Ion Channels: Transient receptor potential

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cys-loop Receptors

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

Ion Channels: Sodium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Western blotting.

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Ion Channels: ATP Receptors

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

Natural killer cell mediated cytotoxicity

Oocyte meiosis

Long-term potentiation

MAPK signaling pathway

T cell receptor signaling pathway

Adherens junction

B cell receptor signaling pathway

Jak-STAT signaling pathway

Focal adhesion

Insulin signaling pathway

Regulation of actin cytoskeleton

Vascular smooth muscle contraction

Wnt signaling pathway

Adipocytokine signaling pathway

Alzheimer's disease

Amyotrophic lateral sclerosis (ALS)

Apoptosis

Axon guidance

Calcium signaling pathway

Chronic myeloid leukemia

Epithelial cell signaling in Helicobacter pylori infection

Leukocyte transendothelial migration

Neurotrophin signaling pathway

Renal cell carcinoma

Riboflavin metabolism

VEGF signaling pathway

Glycine

Metabolic pathways

serine and threonine metabolism

Cell adhesion molecules (CAMs)

Fc gamma R-mediated phagocytosis

Primary immunodeficiency

Tight junction

Endometrial cancer

Glioma

Inositol phosphate metabolism

Long-term depression

Melanoma

Pathways in cancer

Phosphatidylinositol signaling system

Prostate cancer

Small cell lung cancer

TGF-beta signaling pathway

p53 signaling pathway

1

2

Yes

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