Proteins

Proteins

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant Proteins

Native Proteins

Over-expression Lysates

Human

Mouse

ALPHA 1 ANTITRYPSIN

FGF1

BDNF

M-CSF

ANNEXIN A2

TNF ALPHA

GM CSF RECEPTOR ALPHA

IGF1

IL1RA

VEGFA

KALLIKREIN 11

OSTEOPONTIN

EGFR

ERBB 3

FGL1

KALLIKREIN 6

BETA 2 MICROGLOBULIN

G CSF

IL8

FSTL1

GM CSF

IL2

IL4

IL6

NRG1

DECORIN

HYAL1

PCSK9

REG3A

APOLIPOPROTEIN E

CALCITONIN

CLUSTERIN

G PROTEIN ALPHA S

IL1 BETA

IL17

IL22

IL32

INTERFERON GAMMA

MAGP1

MIDKINE

MTMR14

PLAUR

REG3G

SYNAPTOTAGMIN 1

ADIPONECTIN

APOLIPOPROTEIN A I

CCL14

FAM3C

IL1F10

RAB37

SDF1

SERPING1

SONIC HEDGEHOG

CD95

DKK3

EFEMP1

FGF2

HAPTOGLOBIN

IL18

INTERFERON ALPHA 2

KALLIKREIN 8

LTA

PARATHYROID HORMONE RELATED PROTEIN

SYNAPTOTAGMIN 3

CXCL5

FSH BETA

IL1RAP

IL33

LIPF

NEUROPILIN 1

NGAL

NGF

PAI1

PREALBUMIN

RIBONUCLEASE A

ALPHA 2 ANTIPLASMIN

ANGIOGENIN

APOLIPOPROTEIN H

BMP2

CD71

EGFL7

ENKEPHALIN

FGFR2

FOLLISTATIN

GROWTH HORMONE

HTRA1

IL15

IL36RN

IL37

INHIBIN BETA C CHAIN

KGF

LAMC2

LIF

MACROPHAGE INFLAMMATORY PROTEIN 5

MESOTHELIN

MMP1

OIF

ONCOSTATIN M

PIGR

PROLACTIN

IHC

WB

HEK293T

HEK293

E. coli

CHO

Insect

Sf9

Pichia

Baculovirus

C-Myc/DDK

C-MYC/DDK

C-His

Tag Free

N-His

C-6His

C-Fc

N-GST and C-His

N-GST and C-HIS

C-DDK

C-HIS

C-Fc&His

Myc-DDK

C-DDK/His

His-tag

C-8His

N-GST

C-Human Fc

N-His-Trx

C-10His

C-mFc

N-6His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

Tag-free

C-6×His

N-Avi

N-Fc

C-Avi-6His

C-hFc

His-Tag

N-His/DDK

N-8His

N-His-PDCD1(Pro21-Val170)

C-6xHis/AVI

C-AVI

C-AVI&His

C-Avi

C-Avi&6His

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FLAG-Fc

C-Fc-Avi

C-Fc/His

C-Flag,C-6His

C-His&Flag

C-His&HA&AVI

C-Human Fc and 6×His

C-Mouse Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-terminal His

N-6His-Avi

N-AVI&His

N-DDK/His

N-FLAG

N-Fc-FLAG

N-Flag&His

N-HIS

N-His&C-His

N-His&Flag

N-T7

N-Trx-His

N-hFc

N-mFc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

Tag free

Yes

MVPro

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Cytokine-cytokine receptor interaction

Pathways in cancer

Jak-STAT signaling pathway

MAPK signaling pathway

Hematopoietic cell lineage

Chemokine signaling pathway

Complement and coagulation cascades

Toll-like receptor signaling pathway

Focal adhesion

Regulation of actin cytoskeleton

Melanoma

Metabolic pathways

TGF-beta signaling pathway

Natural killer cell mediated cytotoxicity

Autoimmune thyroid disease

Cytosolic DNA-sensing pathway

RIG-I-like receptor signaling pathway

T cell receptor signaling pathway

Allograft rejection

Type I diabetes mellitus

NOD-like receptor signaling pathway

Fc epsilon RI signaling pathway

Graft-versus-host disease

Antigen processing and presentation

Systemic lupus erythematosus

GnRH signaling pathway

ECM-receptor interaction

Apoptosis

Bladder cancer

Asthma

Pancreatic cancer

Neuroactive ligand-receptor interaction

Hedgehog signaling pathway

Hypertrophic cardiomyopathy (HCM)

Endocytosis

Dilated cardiomyopathy

PPAR signaling pathway

Prion diseases

Melanogenesis

Regulation of autophagy

Basal cell carcinoma

Renal cell carcinoma

Wnt signaling pathway

Alzheimer's disease

mTOR signaling pathway

Prostate cancer

Long-term depression

Adipocytokine signaling pathway

p53 signaling pathway

ErbB signaling pathway

Axon guidance

Epithelial cell signaling in Helicobacter pylori infection

Calcium signaling pathway

Neurotrophin signaling pathway

VEGF signaling pathway

Vascular smooth muscle contraction

Colorectal cancer

Ether lipid metabolism

Type II diabetes mellitus

Glioma

Glycerolipid metabolism

Renin-angiotensin system

Arachidonic acid metabolism

Cell adhesion molecules (CAMs)

Gap junction

Small cell lung cancer

Glycerophospholipid metabolism

Primary immunodeficiency

Cell cycle

Amyotrophic lateral sclerosis (ALS)

Linoleic acid metabolism

alpha-Linolenic acid metabolism

Huntington's disease

Lysosome

Oocyte meiosis

Progesterone-mediated oocyte maturation

Chronic myeloid leukemia

Non-small cell lung cancer

Fc gamma R-mediated phagocytosis

Glycosaminoglycan degradation

Adherens junction

Dorso-ventral axis formation

Endometrial cancer

Viral myocarditis

O-Glycan biosynthesis

Proteasome

Taste transduction

Vibrio cholerae infection

Histidine metabolism

Leukocyte transendothelial migration

Amino sugar and nucleotide sugar metabolism

Starch and sucrose metabolism

Acute myeloid leukemia

Purine metabolism

Pyrimidine metabolism

SNARE interactions in vesicular transport

Glycosphingolipid biosynthesis - lacto and neolacto series

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Keratan sulfate biosynthesis

Maturity onset diabetes of the young

N-Glycan biosynthesis

Other glycan degradation

beta-Alanine metabolism

Pathogenic Escherichia coli infection

Glutathione metabolism

Galactose metabolism

Glycosphingolipid biosynthesis - ganglio series

Glycosphingolipid biosynthesis - globo series

Arginine and proline metabolism

Biotin metabolism

Folate biosynthesis

Insulin signaling pathway

Nitrogen metabolism

Riboflavin metabolism

Tryptophan metabolism

Tyrosine metabolism

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Human

E. coli

Human 293

Plasma

Baculovirus

HEK293

Mammalian

P.Pichia

Pancreas

Pituitary

1

2

Yes

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