Proteins

Proteins

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant CytoSections

Mutant Proteins

Over-expression Lysates

Human

FGFR1

AURORA A

JNK1

EGFR

ERBB 3

HER2

TRKB

CAMK2B

AKT1

JNK2

NEK6

ROR1

B RAF

CAMK2D

CSNK1G3

PAK4

CAMKK

FYN

P38

PROTEIN KINASE D2

PYK2

TRKC

CAMKK2

CCK4

FGFR3

MCK10

MET

DDR2

SRC

TIE1

ACTIVIN RECEPTOR TYPE IA

DYSTROPHIA MYOTONICA PROTEIN KINASE

ERK2

FGFR2

MATK

PCTAIRE1

PDGF RECEPTOR BETA

RYK

ABL2

ACTIVIN RECEPTOR TYPE IIB

BMPR2

BMX

CAMK1D

CDK2

CDK6

CK1 EPSILON

CSK

EPH RECEPTOR A2

ERBB 4

FAK

FGFR4

JNK3

MAPKAP KINASE 2

MCSF RECEPTOR

MSK1

PKC BETA 1

PKC EPSILON

PRKACA

STK16

TRKA

ZAK

ACTIVIN RECEPTOR TYPE IIA

AKT3

CAMK2A

HSP22

MEK3

PKC ZETA

RIOK1

ROR2

TGF BETA RECEPTOR II

ACVRL1

AXL

CAMK2G

CHK1

DCAMKL1

EPH RECEPTOR A1

EPH RECEPTOR A3

EPH RECEPTOR A7

FASTK

FGR

FLT1

IGF1 RECEPTOR

INTEGRIN LINKED KINASE

IRAK

IRE1

SGK3

TYRO3

ACTIVIN A RECEPTOR TYPE IC

ALK

BMPR1A

EPH RECEPTOR B3

EPH RECEPTOR B4

ERK1

FLT3

FLT4

GSK3 BETA

HCK

IRAK4

LIM KINASE 2

MARK2

IHC

WB

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

C-Myc/DDK

C-MYC/DDK

N-His

C-His

C-DDK/His

C-DDK

C-Fc

N-GST and C-His

N-GST and C-HIS

His-tag

C-Fc&His

N-His-ABP (Albumin-Binding Protein)

N-GST

C-6His-Avi

C-6His

C-6×His

Myc-DDK

Tag Free

C- 6×His

C-Fc-Avi

C-Myc-DDK

C-hFc

C-mFc

N-10His-Flag

N-FUSION

Yes

MVPro

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

MAPK signaling pathway

Pathways in cancer

Neurotrophin signaling pathway

ErbB signaling pathway

Focal adhesion

GnRH signaling pathway

Chemokine signaling pathway

Endocytosis

Regulation of actin cytoskeleton

Progesterone-mediated oocyte maturation

Insulin signaling pathway

Prostate cancer

Fc epsilon RI signaling pathway

Glioma

Pancreatic cancer

Colorectal cancer

T cell receptor signaling pathway

Calcium signaling pathway

Toll-like receptor signaling pathway

Wnt signaling pathway

Axon guidance

Melanoma

Oocyte meiosis

Adherens junction

Cytokine-cytokine receptor interaction

Long-term potentiation

Non-small cell lung cancer

VEGF signaling pathway

Gap junction

Epithelial cell signaling in Helicobacter pylori infection

Endometrial cancer

Chronic myeloid leukemia

Vascular smooth muscle contraction

Fc gamma R-mediated phagocytosis

Melanogenesis

Bladder cancer

Adipocytokine signaling pathway

Acute myeloid leukemia

mTOR signaling pathway

Renal cell carcinoma

Tight junction

TGF-beta signaling pathway

Natural killer cell mediated cytotoxicity

NOD-like receptor signaling pathway

B cell receptor signaling pathway

RIG-I-like receptor signaling pathway

Type II diabetes mellitus

Long-term depression

Apoptosis

Cell cycle

Olfactory transduction

Leukocyte transendothelial migration

Small cell lung cancer

Hedgehog signaling pathway

Prion diseases

Thyroid cancer

Jak-STAT signaling pathway

Amyotrophic lateral sclerosis (ALS)

p53 signaling pathway

Dorso-ventral axis formation

Alzheimer's disease

Vibrio cholerae infection

Viral myocarditis

Pathogenic Escherichia coli infection

Regulation of autophagy

Cytosolic DNA-sensing pathway

Dilated cardiomyopathy

Hematopoietic cell lineage

Taste transduction

Primary immunodeficiency

Phosphatidylinositol signaling system

Circadian rhythm - mammal

PPAR signaling pathway

Purine metabolism

Ubiquitin mediated proteolysis

Basal cell carcinoma

Hypertrophic cardiomyopathy (HCM)

Nucleotide excision repair

Parkinson's disease

Basal transcription factors

Human

E. coli

Human 293

1

2

Yes

In Stock