Proteins

Proteins

Recombinant Proteins

CytoSections

Mass Spec Standards

Native Proteins

Human

Mouse

Humon

ALPHA 1 ANTITRYPSIN

BDNF

HNF 4 ALPHA

M-CSF

ICAM2

IGF1

JNK1

CD147

EGFR

G CSF

AKT1

BETA CATENIN

CD44

GM CSF

JNK2

LAMP2

CD32B

ICAM1

CD105

SDF1

SONIC HEDGEHOG

CD95

CYSTATIN C

IL6R

TCF4

VCAM1

CD14

CD70

MAP2

PREALBUMIN

SRC

ACTIVIN RECEPTOR TYPE IA

BMP2

CD71

EPCAM

INTEGRIN BETA 1

KGF

LDL RECEPTOR

LIF

ONCOSTATIN M

PDGF RECEPTOR BETA

SMAD2

TCF3 / E2A

TGF BETA 1

CASPASE 3

CD133

CD32A

CD34

CD72

EGF

FGF21

HGF

IL7R ALPHA

JMJD6

LAMP1

PVRL1

AKT3

ALDH1A1

CD16

CD244

CD93

DLK

ER81

FGF19

FLT3 LIGAND

GATA3

GLP1

HBZ

INTEGRIN ALPHA 5

NCR3

PAX3

STAT5B

STAT6

UPA

WIF1

BCL2

CD30

CD4

CD62P

E CADHERIN

GFAP

HNF1 ALPHA

IL10

NEUROFILAMENT

SMAD3

BMPR1A

CARDIAC TROPONIN I

CCN4

CSF2

DKK1

DLL1

ERK1

FIBRONECTIN

FLT3

KLF6

PDGF RECEPTOR ALPHA

SMAD1

AXIN 1

BCL X

BST2

IHC

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Pichia

Baculovirus

C-Myc/DDK

C-MYC/DDK

C-His

N-His

Tag Free

C-Fc

C-6His

C-DDK

N-GST and C-His

C-DDK/His

N-GST and C-HIS

C-Fc&His

His-tag

N-Trx&His

C-6His-Avi

C-6×His

C-mFc

His-Tag

Myc-DDK

N-His-ABP (Albumin-Binding Protein)

C-8His

C-Avi-6His

C-Human Fc

C-hFc

N-6His

N-His-Trx

C- 6×His

C-Flag,C-6His

C-HIS

C-Human Fc and 6×His

C-Mouse Fc

C-Mouse Fc and 6×His

N-6His-Avi

N-AVI&His

N-Avi

N-FC

N-Fc

N-GST

N-His&Flag

Tag-free

MVPro

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Pathways in cancer

Cytokine-cytokine receptor interaction

Focal adhesion

Hematopoietic cell lineage

MAPK signaling pathway

Colorectal cancer

Jak-STAT signaling pathway

Regulation of actin cytoskeleton

Cell adhesion molecules (CAMs)

Melanoma

Wnt signaling pathway

Prostate cancer

Pancreatic cancer

Natural killer cell mediated cytotoxicity

ErbB signaling pathway

TGF-beta signaling pathway

Neurotrophin signaling pathway

Adherens junction

Glioma

T cell receptor signaling pathway

Endocytosis

Endometrial cancer

Leukocyte transendothelial migration

Fc epsilon RI signaling pathway

Progesterone-mediated oocyte maturation

Acute myeloid leukemia

Toll-like receptor signaling pathway

Hypertrophic cardiomyopathy (HCM)

Basal cell carcinoma

Dilated cardiomyopathy

Fc gamma R-mediated phagocytosis

Insulin signaling pathway

Chronic myeloid leukemia

GnRH signaling pathway

Small cell lung cancer

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

ECM-receptor interaction

Epithelial cell signaling in Helicobacter pylori infection

Tight junction

Adipocytokine signaling pathway

B cell receptor signaling pathway

Maturity onset diabetes of the young

Gap junction

Melanogenesis

Non-small cell lung cancer

Apoptosis

Hedgehog signaling pathway

Renal cell carcinoma

VEGF signaling pathway

mTOR signaling pathway

Chemokine signaling pathway

Metabolic pathways

Complement and coagulation cascades

Type II diabetes mellitus

Pathogenic Escherichia coli infection

Systemic lupus erythematosus

Calcium signaling pathway

Bladder cancer

NOD-like receptor signaling pathway

p53 signaling pathway

RIG-I-like receptor signaling pathway

Huntington's disease

Viral myocarditis

Axon guidance

Alzheimer's disease

Autoimmune thyroid disease

Thyroid cancer

Notch signaling pathway

Oocyte meiosis

Amyotrophic lateral sclerosis (ALS)

Cell cycle

Long-term depression

Primary immunodeficiency

Dorso-ventral axis formation

Lysosome

Allograft rejection

Antigen processing and presentation

Type I diabetes mellitus

Graft-versus-host disease

Prion diseases

Renin-angiotensin system

Retinol metabolism

Nicotinate and nicotinamide metabolism

Tyrosine metabolism

Parkinson's disease

Long-term potentiation

Vascular smooth muscle contraction

Asthma

Glycolysis / Gluconeogenesis

Cardiac muscle contraction

Neuroactive ligand-receptor interaction

PPAR signaling pathway

Phosphatidylinositol signaling system

Vibrio cholerae infection

Cysteine and methionine metabolism

Phenylalanine

Phenylalanine metabolism

Purine metabolism

Pyrimidine metabolism

Starch and sucrose metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

tyrosine and tryptophan biosynthesis

Amino sugar and nucleotide sugar metabolism

Drug metabolism - cytochrome P450

Drug metabolism - other enzymes

Fructose and mannose metabolism

Glutathione metabolism

Inositol phosphate metabolism

Linoleic acid metabolism

Metabolism of xenobiotics by cytochrome P450

Oxidative phosphorylation

Primary bile acid biosynthesis

Regulation of autophagy

Ubiquitin mediated proteolysis

ABC transporters

Citrate cycle (TCA cycle)

Pyruvate metabolism

Galactose metabolism

Human

E. coli

Human 293

Plasma

1

2

Yes

In Stock