United States
Germany
Japan
United Kingdom
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Calmodulin bovine protein, 1 mg
Recombinant Proteins
Vimentin bovine protein, 0.1 mg
SRY / TDF bovine recombinant protein, 10 µg
| Expression Host | E. coli |
FGF basic / FGF2 bovine protein, 50 µg
| Expression Host | E. coli |
Vimentin bovine protein, 0.25 mg
RNASE1 bovine protein, 0.1 g
Thyroglobulin bovine protein, 0.1 g
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- 10 mM sodium phosphate (2)
- 2 mM dithiothreitol (2)
- ELISA. (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
-
ELISA.
Functional Assays. (1)
