Recombinant Proteins

Proteins

Recombinant Proteins

Human

Mouse

SIRP ALPHA

ACID PHOSPHATASE

PPM1A

PPM1G

SHP1

MTMR14

MTMR2

PPM1B

PTPRS

SKIP

PHOSPHOSERINE PHOSPHATASE

PP1C GAMMA

PPP2R5D

PTP4A3

CTDSP1

DUSP13

DUSP4

GLEPP1

PCPTP1

PDP1

PPP2R4

PTP ALPHA

PTP EPSILON

PTPDC1

PTPN5

PTPN7

STYX

SYNAPTOJANIN

PROSTATIC ACID PHOSPHATASE

PTEN

PTP1B

CALCINEURIN B

CD45

CTDSPL

DUSP3

PTP4A2

PTPN11

SHP2

CDC25A

CDC25C

DAPP1

DEP1

DUSP12

DUSP14

DUSP15

DUSP18

DUSP19

DUSP23

DUSP27

DUSP6

DUSP9

HCE

ILKAP

LIPRIN ALPHA 1

MTMR1

MTMR6

MTMR7

MTMR8

MTMR9

PDP2

PLASTICITY RELATED GENE 3

PNK

PP2A-ALPHA

PP4R1

PPM1D

PPM1F

PPM1H

PPM1J

PPM1K

PPM1L

PPP1A

PPP1R1C

PPP1R7

PPP1R9B

PPP2R1A

PPP2R5B

PPP2R5C

PPP3CC

PPP3R2

PROTEIN PHOSPHATASE 1 BETA

PTP KAPPA

PTP4A1

PTPD1

PTPN12

PTPN18

PTPN20B

PTPRB

PTPRN2

SET

SSH3

SYNAPTOJANIN 2

TCPTP

DUSP10

PLEIOTROPHIN

PRY

CALCINEURIN A

DUSP11

DUSP22

DUSP26

DUSP5

HEK293T

E. coli

HEK293

Sf9

C-Myc/DDK

N-His

C-His

N-GST and C-His

C-6His

N-His&C-His

C-6His-Avi

C-DDK

C-DDK/His

N-HIS

N-His-ABP (Albumin-Binding Protein)

Tag Free

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

P450

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Potassium

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

ELISA.
Western blotting.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Functional Assays.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

MAPK signaling pathway

Oocyte meiosis

Metabolic pathways

Riboflavin metabolism

Adherens junction

Wnt signaling pathway

Natural killer cell mediated cytotoxicity

Insulin signaling pathway

T cell receptor signaling pathway

B cell receptor signaling pathway

Long-term potentiation

Inositol phosphate metabolism

Phosphatidylinositol signaling system

Focal adhesion

Fructose and mannose metabolism

Jak-STAT signaling pathway

Thiamine metabolism

Regulation of actin cytoskeleton

Vascular smooth muscle contraction

Tight junction

Alzheimer's disease

Amyotrophic lateral sclerosis (ALS)

Apoptosis

Axon guidance

Calcium signaling pathway

VEGF signaling pathway

Adipocytokine signaling pathway

Chronic myeloid leukemia

Epithelial cell signaling in Helicobacter pylori infection

Leukocyte transendothelial migration

Neurotrophin signaling pathway

Renal cell carcinoma

p53 signaling pathway

Glycine

Long-term depression

TGF-beta signaling pathway

serine and threonine metabolism

Cell cycle

Progesterone-mediated oocyte maturation

Cell adhesion molecules (CAMs)

Endometrial cancer

Fc gamma R-mediated phagocytosis

Glioma

Melanoma

Pathways in cancer

Primary immunodeficiency

Prostate cancer

Small cell lung cancer

Type I diabetes mellitus

Human

E. coli

Human 293

1

2

Yes

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