Recombinant Proteins

Proteins

Recombinant Proteins

Human

Mouse

Humon

ALPHA 1 ANTITRYPSIN

IGF1

EGFR

ICAM2

M-CSF

GM CSF

HNF 4 ALPHA

CD147

G CSF

ICAM1

LAMP2

BDNF

CD44

JNK1

SDF1

SONIC HEDGEHOG

AKT1

BETA CATENIN

CYSTATIN C

BMP2

CD105

CD70

JNK2

PREALBUMIN

TGF BETA 1

VCAM1

CD32B

CD95

EPCAM

IL6R

KGF

LIF

ONCOSTATIN M

PDGF RECEPTOR BETA

TCF4

CD14

CD32A

CD72

EGF

FGF21

IL7R ALPHA

LAMP1

NCR3

SRC

ACTIVIN RECEPTOR TYPE IA

ALDH1A1

CD16

CD30

CD34

CD71

CD93

CSF2

DLK

FGF19

FLT3 LIGAND

GLP1

HBZ

INTEGRIN ALPHA 5

LDL RECEPTOR

PVRL1

STAT5B

STAT6

TCF3 / E2A

UPA

WIF1

AKT3

CASPASE 3

CD133

CD244

CD62P

DKK1

E CADHERIN

FIBRONECTIN

GATA3

HGF

HNF1 ALPHA

IL10

INTEGRIN BETA 1

JMJD6

KDR

MAP2

NEUROFILAMENT

SMAD2

BCL2

BMPR1A

CARDIAC TROPONIN I

CD117

CD16B

CD38

CD4

CD73

DLL1

FLT3

GFAP

KLF6

PDGF RECEPTOR ALPHA

PROCR

SMAD3

VEGF RECEPTOR 2

BST2

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Pichia

Baculovirus

C-Myc/DDK

C-His

N-His

Tag Free

C-Fc

C-6His

C-DDK

N-GST and C-His

C-DDK/His

N-GST and C-HIS

C-Fc&His

His-tag

N-Trx&His

C-6His-Avi

C-6×His

C-mFc

His-Tag

Myc-DDK

N-His-ABP (Albumin-Binding Protein)

C-8His

C-Avi-6His

C-Human Fc

C-hFc

N-6His

N-His-Trx

C- 6×His

C-Flag,C-6His

C-HIS

C-Human Fc and 6×His

C-Mouse Fc

C-Mouse Fc and 6×His

N-6His-Avi

N-AVI&His

N-Avi

N-FC

N-Fc

N-GST

N-His&Flag

Tag-free

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

P450

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

ELISA

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Pathways in cancer

Cytokine-cytokine receptor interaction

Focal adhesion

Hematopoietic cell lineage

MAPK signaling pathway

Jak-STAT signaling pathway

Regulation of actin cytoskeleton

Colorectal cancer

Cell adhesion molecules (CAMs)

Melanoma

Natural killer cell mediated cytotoxicity

Prostate cancer

Pancreatic cancer

ErbB signaling pathway

Wnt signaling pathway

Endocytosis

Glioma

T cell receptor signaling pathway

TGF-beta signaling pathway

Leukocyte transendothelial migration

Adherens junction

Endometrial cancer

Fc epsilon RI signaling pathway

Progesterone-mediated oocyte maturation

Hypertrophic cardiomyopathy (HCM)

Neurotrophin signaling pathway

Acute myeloid leukemia

Dilated cardiomyopathy

Chronic myeloid leukemia

Basal cell carcinoma

Fc gamma R-mediated phagocytosis

Toll-like receptor signaling pathway

ECM-receptor interaction

GnRH signaling pathway

Gap junction

Epithelial cell signaling in Helicobacter pylori infection

Small cell lung cancer

Insulin signaling pathway

Non-small cell lung cancer

Tight junction

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

B cell receptor signaling pathway

Hedgehog signaling pathway

Maturity onset diabetes of the young

Apoptosis

VEGF signaling pathway

mTOR signaling pathway

Adipocytokine signaling pathway

Chemokine signaling pathway

Renal cell carcinoma

Melanogenesis

Metabolic pathways

Complement and coagulation cascades

Pathogenic Escherichia coli infection

Systemic lupus erythematosus

Calcium signaling pathway

Type II diabetes mellitus

Bladder cancer

p53 signaling pathway

Viral myocarditis

Autoimmune thyroid disease

NOD-like receptor signaling pathway

RIG-I-like receptor signaling pathway

Axon guidance

Oocyte meiosis

Alzheimer's disease

Cell cycle

Dorso-ventral axis formation

Long-term depression

Lysosome

Notch signaling pathway

Primary immunodeficiency

Thyroid cancer

Amyotrophic lateral sclerosis (ALS)

Huntington's disease

Allograft rejection

Type I diabetes mellitus

Antigen processing and presentation

Graft-versus-host disease

Nicotinate and nicotinamide metabolism

Renin-angiotensin system

Retinol metabolism

Prion diseases

Tyrosine metabolism

Asthma

Parkinson's disease

Cardiac muscle contraction

Neuroactive ligand-receptor interaction

Purine metabolism

Pyrimidine metabolism

ABC transporters

Cysteine and methionine metabolism

Glutathione metabolism

Long-term potentiation

Phenylalanine

Phenylalanine metabolism

Phosphatidylinositol signaling system

Ubiquinone and other terpenoid-quinone biosynthesis

Vascular smooth muscle contraction

Vibrio cholerae infection

tyrosine and tryptophan biosynthesis

Amino sugar and nucleotide sugar metabolism

Drug metabolism - cytochrome P450

Drug metabolism - other enzymes

Fructose and mannose metabolism

Glycolysis / Gluconeogenesis

Inositol phosphate metabolism

Linoleic acid metabolism

Metabolism of xenobiotics by cytochrome P450

Oxidative phosphorylation

PPAR signaling pathway

Primary bile acid biosynthesis

Regulation of autophagy

Starch and sucrose metabolism

Ubiquitin mediated proteolysis

Human

E. coli

Human 293

1

2

Yes

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