Recombinant Proteins

Proteins

Recombinant Proteins

Mutant Proteins

Human

Mouse

Humon

ALPHA 1 ANTITRYPSIN

FGF1

ALPHA SYNUCLEIN

FGFR1

TNF ALPHA

HMGA1

PRKAR1B

TPM1

VEGFA

IGF1

MAX

ALDOLASE

BETA 2 MICROGLOBULIN

CATHEPSIN B

EGFR

HMG1

IL8

M-CSF

S100 CALCIUM BINDING PROTEIN A13

ASPARTATE BETA HYDROXYLASE

CD47

CEACAM1

GM CSF

HER2

HNF 4 ALPHA

IL2

IL4

P38

ACRV1

ALKALINE PHOSPHATASE

ANNEXIN A2

AURORA A

CARBONIC ANHYDRASE X

CD36

CD3E

ERBB 3

FGL1

IL1RA

IL6

OSTEOPONTIN

PD-L1

PTPRN

APOLIPOPROTEIN E

ARSA

B RAF

CARDIAC TROPONIN T

CD10

CD147

ESTROGEN RELATED RECEPTOR GAMMA

FHL2

FKBP2

G CSF

GALE

GM CSF RECEPTOR ALPHA

HNRNPD

IL22

INTERFERON GAMMA

KALLIKREIN 11

KALLIKREIN 6

LAMP2

LIVER CARBOXYLESTERASE 1

PKM2

ROR1

SIRP ALPHA

SYNTENIN

TAU

APOLIPOPROTEIN A I

BDNF

CALCITONIN

CD44

DGKA

DOUBLECORTIN

GMPR2

HOMER2

IKK GAMMA

IL1 BETA

JNK1

LMO2

MPST

P53

PPAR GAMMA

RAB27A

RNF14

SDF1

SONIC HEDGEHOG

SUMO 3

TRKB

ADIPONECTIN

AKT1

APE1

BETA CATENIN

CLUSTERIN

CTLA4

CYSTATIN C

DECORIN

FBP1

FGF2

FGFR3

HIP2

IL18

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Pichia

Baculovirus

C-Myc/DDK

C-His

N-His

Tag Free

C-Fc

N-GST and C-His

C-DDK

C-6His

C-DDK/His

N-GST and C-HIS

N-GST

His-tag

N-His-ABP (Albumin-Binding Protein)

C-Fc&His

C-6His-Avi

Myc-DDK

C-HIS

C-mFc

N-His&C-His

N-6His

C-8His

N-His-Trx

N-Trx&His

C-10His

C-Human Fc

C-hFc

C-Fc-Avi

N-GST&His

N-His&SUMO

C-Avi&6His

N-Fc

Tag-free

C-Avi-6His

C-MYC/DDK

C-Mouse Fc

C-6xHis

N-HIS

N-His&C-Fc

N-His&T7

N-MARI

N-T7&C-His

N-Trx

C-Fc&AVI

C-hFc&His

N-8His

N-His/DDK

C-Fc/His

C-Flag

C-HUMAN

N-6His-Avi

N-GST, C-His

N-His&Flag

N-His-PDCD1(Pro21-Val170)

C-AVI&His

C-AVI-PLUS

C-Avi

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FC-6HIS&C-FC-Flag

C-FLAG-Fc

C-Fc-Flag&C-Fc-6His

C-Flag,C-6His

C-GST

C-HIS/DDK

C-hIgG2 Fc

C-hIgG4 Fc

C-mFC

N-6His-FLAG

N-6His-Flag

N-AVI

N-AVI&His

N-Avi

N-Avi&6His

N-DDK

N-DDK/His

N-FC

N-FLAG

N-FUSION

N-Fc-FLAG

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-His-GST

N-Human Fc

N-T7

N-TRX

N-TRXA

N-hFc

N-mFc

N-terminal His

Tag free

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

P450

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Potassium

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

ELISA.
Western blotting.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Functional Assays.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Metabolic pathways

Cytokine-cytokine receptor interaction

Pathways in cancer

MAPK signaling pathway

Focal adhesion

Jak-STAT signaling pathway

Chemokine signaling pathway

Regulation of actin cytoskeleton

Hematopoietic cell lineage

Neurotrophin signaling pathway

T cell receptor signaling pathway

Toll-like receptor signaling pathway

Natural killer cell mediated cytotoxicity

Insulin signaling pathway

Endocytosis

ErbB signaling pathway

Prostate cancer

Pancreatic cancer

Apoptosis

Cell adhesion molecules (CAMs)

Melanoma

Axon guidance

Cell cycle

Colorectal cancer

Progesterone-mediated oocyte maturation

Fc epsilon RI signaling pathway

Wnt signaling pathway

Adherens junction

Renal cell carcinoma

TGF-beta signaling pathway

GnRH signaling pathway

Leukocyte transendothelial migration

Calcium signaling pathway

Glioma

Alzheimer's disease

Chronic myeloid leukemia

NOD-like receptor signaling pathway

B cell receptor signaling pathway

Oocyte meiosis

Purine metabolism

RIG-I-like receptor signaling pathway

Small cell lung cancer

Bladder cancer

Ubiquitin mediated proteolysis

Adipocytokine signaling pathway

VEGF signaling pathway

Gap junction

Tight junction

Hypertrophic cardiomyopathy (HCM)

Lysosome

Dilated cardiomyopathy

Epithelial cell signaling in Helicobacter pylori infection

Complement and coagulation cascades

Acute myeloid leukemia

Endometrial cancer

p53 signaling pathway

Non-small cell lung cancer

Fc gamma R-mediated phagocytosis

Melanogenesis

Long-term potentiation

Glycolysis / Gluconeogenesis

Phosphatidylinositol signaling system

Amyotrophic lateral sclerosis (ALS)

Vascular smooth muscle contraction

ECM-receptor interaction

PPAR signaling pathway

Autoimmune thyroid disease

Long-term depression

Cytosolic DNA-sensing pathway

Huntington's disease

Type I diabetes mellitus

Antigen processing and presentation

Neuroactive ligand-receptor interaction

Type II diabetes mellitus

mTOR signaling pathway

Allograft rejection

Inositol phosphate metabolism

Pathogenic Escherichia coli infection

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Thyroid cancer

Arginine and proline metabolism

Viral myocarditis

Graft-versus-host disease

Pyrimidine metabolism

Prion diseases

Systemic lupus erythematosus

Fructose and mannose metabolism

Tyrosine metabolism

Metabolism of xenobiotics by cytochrome P450

Primary immunodeficiency

Glycerolipid metabolism

Drug metabolism - cytochrome P450

Parkinson's disease

Retinol metabolism

Amino sugar and nucleotide sugar metabolism

Hedgehog signaling pathway

Regulation of autophagy

Valine

Base excision repair

Drug metabolism - other enzymes

Galactose metabolism

Arachidonic acid metabolism

Nitrogen metabolism

Pyruvate metabolism

leucine and isoleucine degradation

Tryptophan metabolism

Glycerophospholipid metabolism

Fatty acid metabolism

Asthma

Glutathione metabolism

Cardiac muscle contraction

Basal cell carcinoma

Nucleotide excision repair

Olfactory transduction

Pentose phosphate pathway

Starch and sucrose metabolism

Butanoate metabolism

Alanine

Cysteine and methionine metabolism

Sphingolipid metabolism

aspartate and glutamate metabolism

Androgen and estrogen metabolism

Proteasome

Renin-angiotensin system

Vibrio cholerae infection

Porphyrin and chlorophyll metabolism

Histidine metabolism

Propanoate metabolism

Dorso-ventral axis formation

Maturity onset diabetes of the young

SNARE interactions in vesicular transport

Lysine degradation

Phenylalanine metabolism

Linoleic acid metabolism

Riboflavin metabolism

beta-Alanine metabolism

Mismatch repair

Notch signaling pathway

Glycosaminoglycan degradation

Citrate cycle (TCA cycle)

Other glycan degradation

Glycine

serine and threonine metabolism

Ether lipid metabolism

Selenoamino acid metabolism

DNA replication

Aminoacyl-tRNA biosynthesis

Homologous recombination

Oxidative phosphorylation

Spliceosome

Taste transduction

C21-Steroid hormone metabolism

Ribosome

Folate biosynthesis

Pantothenate and CoA biosynthesis

Primary bile acid biosynthesis

ABC transporters

Synthesis and degradation of ketone bodies

Terpenoid backbone biosynthesis

Glyoxylate and dicarboxylate metabolism

Nicotinate and nicotinamide metabolism

Pentose and glucuronate interconversions

Basal transcription factors

Circadian rhythm - mammal

Ascorbate and aldarate metabolism

One carbon pool by folate

Steroid biosynthesis

Limonene and pinene degradation

Thiamine metabolism

Methane metabolism

Non-homologous end-joining

alpha-Linolenic acid metabolism

Glycosphingolipid biosynthesis - globo series

RNA degradation

Ubiquinone and other terpenoid-quinone biosynthesis

leucine and isoleucine biosynthesis

Caffeine metabolism

Glycosphingolipid biosynthesis - ganglio series

Phenylalanine

tyrosine and tryptophan biosynthesis

Sulfur metabolism

Taurine and hypotaurine metabolism

Fatty acid elongation in mitochondria

Glycosphingolipid biosynthesis - lacto and neolacto series

Protein export

Biosynthesis of unsaturated fatty acids

Fatty acid biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

N-Glycan biosynthesis

O-Glycan biosynthesis

Vitamin B6 metabolism

D-Arginine and D-ornithine metabolism

Heparan sulfate biosynthesis

RNA polymerase

Biotin metabolism

Chondroitin sulfate biosynthesis

D-Glutamine and D-glutamate metabolism

Keratan sulfate biosynthesis

Human

E. coli

Human 293

Baculovirus

CHO Stable

HEK293

Mammalian

P.Pichia

1

2

Yes

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