Human IgM mouse monoclonal antibody, clone M15/8, Purified
| Applications | Flow Cytometry: 20 µg/ml. Immunohistochemistry on frozen sections: 20 µg/ml. ELISA: 5 µg/ml as coating Antibody. Immunoprecipitation. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 5,159.00
4周
Rat IgM Mu chain specific F(ab’)2 Fragment mouse monoclonal antibody, clone M 2A1, Purified
| Applications | B Cell Stimulation: 5 µg per mL of tissue culture media. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, APC
| Applications | Flow Cytometry: < / = 0.2 µg/10e6 cells. Identification and enumeration of IgE+ cells. Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells. Also used as a second step reagent for mouse IgM monoclonal antibodies. |
| Reactivities | Mouse |
| Conjugation | APC |
CNY 4,026.00
4周
Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC
| Applications | FLISA: 1/200-1/400. Flow Cytometry: ≤ 1 µg/106 cells. Identification and enumeration of IgG2a+ cells. Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells. Also used as a second step reagent for mouse IgG2a monoclonal antibodies. |
| Reactivities | Mouse |
| Conjugation | FITC |
CNY 15,460.00
5周
Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites
| Applications | Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000-1/10000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 15,460.00
5周
Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites
| Applications | Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000 Upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 9,630.00
5周
Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC
| Applications | To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. General Recommended Dilutions: Histochemical Use: 1/1-1/50. ELISA: from 1/20 upwards. Western blot: from 1/40 upwards. |
| Reactivities | Human |
| Conjugation | TRITC |
CNY 6,190.00
5周
Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites
| Applications | To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 7,710.00
5周
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Purified
| Applications | To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 9,630.00
5周
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP
| Applications | To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/10-1/100. ELISA: from 1/100 upwards. Western blot: from 1/250 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Ascites
| Applications | To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 6,190.00
5周
Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Purified
| Applications | To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/200 upwards. Western blot: from 1/500 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 9,630.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC
| Applications | To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques. General Recommended Dilutions: Histochemical Use: 1/10-1/100 |
| Reactivities | Human |
| Conjugation | TRITC |
Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified
| Applications | Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
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