Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]

Specifications

Product Data
Product Name	Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]
Clone Name	NI 194-4 (A89-039)
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques. General Recommended Dilutions: Histochemical Use: 1/10-1/100
Reactivity	Human
Host	Mouse
Immunogen	Highly purified Secretory component isolated from Human milk.
Formulation	PBS, pH 7.2 without preservatives. No foreign protein added. Label: TRITC State: Lyophilized purified IgG fraction. Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm / 573 nm Molar radio: Fluorochrome/IgG: ~ 1.8
Concentration	0.4 mg/ml
Conjugation	TRITC
Storage Condition	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.
Note	Fluorescent Marker: Tetramethylrhodamine isothiocyanate isomer R with an Orange-Red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. Conjugation procedure: Conjugation is carried out using a proprietary technique for the binding of TRITC, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.

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