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Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone MA2, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Secondary Antibodies
Human IgM mouse monoclonal antibody, clone M15/8, Purified
| Applications | Flow Cytometry: 20 µg/ml. Immunohistochemistry on frozen sections: 20 µg/ml. ELISA: 5 µg/ml as coating Antibody. Immunoprecipitation. |
| Reactivities | Human |
| Conjugation | Unconjugated |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 8,794.00
5周
Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC
| Applications | To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. General Recommended Dilutions: Histochemical Use: 1/1-1/50. ELISA: from 1/20 upwards. Western blot: from 1/40 upwards. |
| Reactivities | Human |
| Conjugation | TRITC |
CNY 8,794.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC
| Applications | To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques. General Recommended Dilutions: Histochemical Use: 1/10-1/100 |
| Reactivities | Human |
| Conjugation | TRITC |
Human IgG (H&L) Antibody
| Applications | WB: 1:500 - 1:5,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:5,000 - 1:50,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H&L) Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:50,000 |
| Reactivities | Human |
| Conjugation | HRP |
CNY 14,063.00
5周
Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites
| Applications | Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000-1/10000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 14,063.00
5周
Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites
| Applications | Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000 Upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone I27/mAb27
| Applications | FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified
| Applications | Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM mouse monoclonal antibody, clone NI 179, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/250 upwards. Western blot: from 1/500 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 8,794.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), FITC
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques Immunofluorescence staining techinqiues. General Recommended Dilutions: Histochemical Use: from 1/10 upwards. |
| Reactivities | Human |
| Conjugation | FITC |
CNY 8,794.00
5周
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), HRP
| Applications | To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 7,161.00
5周
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Purified
| Applications | To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
