Secondary Antibodies

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Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]
AM20254HR-N

CNY 9,630.00

5周

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/100 upwards.
Western blot: from 1/250 upwards.
Reactivities Human
Conjugation HRP
Human IgA2 Mouse Monoclonal Antibody [Clone ID: NI 512 (A89-038)]
AM20247AF-N

CNY 6,190.00

5周

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Ascites

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgA2 (A2m2 Allotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 194-3 (A89-040)]
AM20248PU-N

CNY 6,190.00

5周

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Purified

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated
IGHG1 (heavy chain) Mouse Monoclonal Antibody [Clone ID: IG266]
AM50285PU-S

CNY 5,830.00

5周

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated
Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]
AM20261TC-N

CNY 9,630.00

5周

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC

Applications To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC
Human IgG4 Mouse Monoclonal Antibody [Clone ID: MRQ-44]
AM33493PU-N

CNY 16,013.00

5周

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated
Chicken IgG / Chicken IgY Mouse Monoclonal Antibody [Clone ID: 9B4]
AM00074PU-N

CNY 4,224.00

5周

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1-10 µg.
Reactivities Chicken
Conjugation Unconjugated
Human IgA (Fc specific) Mouse Monoclonal Antibody [Clone ID: 15D6]
BM469FS

CNY 5,225.00

4周

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000.
Immunofluorescence.
Reactivities Human
Conjugation FITC
Human IgG3 (Hinge Region) Mouse Monoclonal Antibody [Clone ID: HP6050]
SM1026P

CNY 3,113.00

4周

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Azide Free

Applications ELISA.
FLISA: 1 µg/ml.
Western blotting.
Dot- and Slot-Immunoblotting
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated
Human IgG Mouse Monoclonal Antibody [Clone ID: 4D2D9G8]
AM06072PU-N

CNY 3,432.00

5周

Human IgG mouse monoclonal antibody, clone 4D2D9G8, Purified

Applications Western Blot: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Reactivities Human
Conjugation Unconjugated
Chicken IgA (alpha chain specific) Mouse Monoclonal Antibody [Clone ID: A-1]
AM08128PU-N

CNY 4,862.00

4周

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated
Rat IgG2b (Gamma-2b chain specific) Mouse Monoclonal Antibody [Clone ID: 2B 10A8]
AM08093FC-N

CNY 4,697.00

4周

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC
Rat IgG2a (gamma 2a chain specific) Mouse Monoclonal Antibody [Clone ID: 2A 8F4]
AM08092RP-N

CNY 4,466.00

4周

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation PE