Il23a (NM_031252) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	Sf9
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Mouse IL-23 consists of two subunits: P19, the region of Met1-Ala196 from gene Accession# NM_031252, and P40, the region of Met1-Ser 335 from gene Accession# NM_008352, linked via a disulphide bond:
Tag	Tag Free
Predicted MW	55.4 kDa
Concentration	lot specific
Purity	Purity is >95%, as determined by Coomassie stained SDS-PAGE.
Buffer	1 x PBS
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 10^6 units/mg, determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_112542
Locus ID	83430
UniProt ID	Q9EQ14
Refseq Size	1359
Cytogenetics	10 D3
Refseq ORF	591
Synonyms	IL-23; p19
Summary	Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.[UniProtKB/Swiss-Prot Function]

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