Epo (NM_007942) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Mouse EPO, the region of Ala27-Arg192, from gene Accession# NM_007942.2
Tag	Tag Free
Predicted MW	18.6 kDa
Concentration	lot specific
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Buffer	1 x PBS
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2 x 10^6 units/mg, determined by the dose dependent stimulation of TF-1 cells proliferation.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_031968
Locus ID	13856
UniProt ID	P07321, Q0VED9
Refseq Size	715
Cytogenetics	5 76.5 cM
Refseq ORF	579
Summary	This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

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