PIN1 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Predicted MW	18.2 kDa
Concentration	lot specific
Purity	>95% by SDS-PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer, pH 7.5 containing 0.1M NaCl, 5 mM DTT and 20% Glycerol
Preparation	Liquid purified protein
Applications	Protocol: Activity Assay 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2. Add 10 ul of recombinant PIN 1 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25°C.
Protein Description	Recombinant human PIN1 was expressed in E.coli and purified by conventional chromatography techniques.
Storage	Store the antigen at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific: > 330 nmoles/min/mg, defined as the amount of enzyme that cleaves 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
Reference Data
RefSeq	NP_006212
Locus ID	5300
UniProt ID	Q13526
Cytogenetics	19p13.2
Synonyms	DOD; UBL5
Summary	Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
Protein Families	Druggable Genome
Protein Pathways	RIG-I-like receptor signaling pathway

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