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E1 (NC_001693) Virus Tagged ORF Clone
CAT＃: VC101980
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043439
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CNY 7,320.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101980 represents NCBI reference of NP_043439 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCCCAATAAAGGGATCAATTCTCTGGAATTGAACGAAGGCCATAGCGAATGGTATGTAGTTA CCGAGGCAGAGTGTATTAACTCTCTGGACACCATGGAGGAACTCTTTGAAGAAAGCACTGACGGCTCCAT AGTATCCAATCTCATCGACGACAGTGAAGAGCTGGAAGAAGGGAATAGCCTGGCCCTGTACAATGAGCAG CTGACTGAAGATTGCAATCGCGCCATTTTGGCGCTTAAAAGAAAACTTACTAAAACTCCCTTGAAGAGCC AGGACCGGACAGTTGCCGATCTTTCTCCACGCTTGGAAGCCGTTACAATATCCCCACAACGGCAAAGCAA GCGGAGGTTGTTCGAGGACTCCGGGCTGGGCGAAGATGAGGCAACCAATAGCATCGAGAAAAAGGTGGTA TCAAACTCCCTGGAGTCAAACGAGAGTGGCACACTTGTGGTAGAGACAGACTCCATTTTTAGGAGTACCA ATAGAAAGGCCACTCTGCTGGCGAAGTTCAAGGAATACTTCGGCGTAGCCTATGGGGATCTTACTAGACC GTTTAAGTCCGATAGGAGTTGTTGCGAGAATTGGGTGATCAGCGTCTGCGCCGCTGCTGAAGAGGTCATT GAAGCATCTAAAACAGTCATGCAACAGCACTGCGATTTCCTGCAGGTTATTTCTTATGGCTTTTACGCTC TCTACCTTGTGAAGTTCAAAACCGCAAAGAGTCGAGATACTATCATGAAACTGTTCTCCCTCACACTCAA TGTACAGGAGCAACAATTGATGTGTGATCCTCCAAAAAGCCGAAGTACTCCCACCGCCCTTTATTTCTAC AGACGATCCTTTGGCAATGCCTCATTTATCTACGGCCCCTTCCCTGATTGGCTTGCCAAGCTGACGATGC TGGATCACGAGTCCGCCGCATCCTCCGAGCAATTCGAGCTCGCCCAGATGATCCAGTTTGCATACGATAA CAACCTGACCACAGAGAGCGAAATCGCTTACAAGTATGCGCTGCTGGCTGATTCCGATGCGAATGCAGCA GCCTTCTTGAAGAGCAATCAGCAAGTGAAATACGTCCGCGACTGCTACGCGATGCTGAGGTATTACAAGA GACAGGAGATGAAAGACATGTCTATATCAGAGTGGATCTGGAAATGCTGTGATGACTGCAACCAGGAAGG AAACTGGAAGCTCATTGCCCAGTTTCTGCGCTACCAGGAGGTGAATTTTATCAGCTTTCTCTGTGCTCTT AAAACACTTTTTAAGGGCATACCAAAACGAAACTGTCTCGTTTTCTGGGGGCCGCCCGATACGGGCAAGA GCTATATTTGCAGCTCTCTCACCAGGTTTATGCAAGGTAAGGTGGTGAGTTTCATGAATAGACACTCACA GTTCTGGCTTCAGCCCCTTCAGGATTGTAAACTTGGTTTTCTTGACGACGCCACATTCCAGTGCTGGCAG TATATGGATGTCAACATGCGGAATGCTTTGGATGGAAACCACATCTCTCTCGACCTGAAGCATAAAGCTC CTCTGCAGATCAAGCTGCCCCCTCTCCTGATCACCACAAATGTGGACGTTGAGAACGAGGCCTCTCTGAT GTATTTGAAAAGCCGCCTGGTTTTTTTCAAGTTCCCCAACAAACTGCCCCTGAAGGAAAATGACGAGGTC CTTTACGAGATTACTGATGCCAGTTGGAAGTGCTTCTTCATTAAATTTGCTAGCCACCTTGAACTCACAG CCAGAGGCGATGAACAGCATGAAAGCGGCCGCAGTGACAGGGCTTTCAGGTGCACCGCCGGCACAAATAC TGAGAGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101980 representing NP_043439
Red=Cloning sites Green=Tags MADPNKGINSLELNEGHSEWYVVTEAECINSLDTMEELFEESTDGSIVSNLIDDSEELEEGNSLALYNEQ LTEDCNRAILALKRKLTKTPLKSQDRTVADLSPRLEAVTISPQRQSKRRLFEDSGLGEDEATNSIEKKVV SNSLESNESGTLVVETDSIFRSTNRKATLLAKFKEYFGVAYGDLTRPFKSDRSCCENWVISVCAAAEEVI EASKTVMQQHCDFLQVISYGFYALYLVKFKTAKSRDTIMKLFSLTLNVQEQQLMCDPPKSRSTPTALYFY RRSFGNASFIYGPFPDWLAKLTMLDHESAASSEQFELAQMIQFAYDNNLTTESEIAYKYALLADSDANAA AFLKSNQQVKYVRDCYAMLRYYKRQEMKDMSISEWIWKCCDDCNQEGNWKLIAQFLRYQEVNFISFLCAL KTLFKGIPKRNCLVFWGPPDTGKSYICSSLTRFMQGKVVSFMNRHSQFWLQPLQDCKLGFLDDATFQCWQ YMDVNMRNALDGNHISLDLKHKAPLQIKLPPLLITTNVDVENEASLMYLKSRLVFFKFPNKLPLKENDEV LYEITDASWKCFFIKFASHLELTARGDEQHESGRSDRAFRCTAGTNTESI TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001693 |
| ORF Size | 1830 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_001693.1, NP_043439 |
| RefSeq ORF | 1830 bp |
| Locus ID | 1403640 |
| MW | 69.9 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101978 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043437 |
CNY 3,800.00 |
|
| VC101979 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043438 |
CNY 3,800.00 |
|
| VC101981 | Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043440 |
CNY 4,750.00 |
|
| VC101982 | Myc-DDK-tagged ORF clone of viral ORF for putative, partial [Human papillomavirus type 60], codon optimized for human cell expression, NP_043441 |
CNY 3,800.00 |
|
| VC101983 | Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043442 |
CNY 6,370.00 |
|
| VC101984 | Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 60], codon optimized for human cell expression, NP_043443 |
CNY 6,180.00 |
