VASH2 (NM_001301056) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335577 representing NM_001301056. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGGCTCCGCGGCCGACACTCACCGCTGCCCCCACCCCAAAGGCGCCAAAGGCACCCGGTCCCGG AGCAGCCACGCGCGGCCCGTGAGCCTCGCCACCAGCGGGGGCTCAGAGGAGGAGGACAAAGACGGCGGG GTGCTGTTCCACGTCAACAAGAGCGGCTTCCCCATCGACAGCCACACCTGGGAGCGCATGTGGATGCAC GTGGCCAAGGTGCACCCTAAGGGGGGAGAAATGGTGGGCGCCATCAGGAACGCCGCCTTCTTGGCAAAG CCTTCAATACCCCAGGTCCCAAACTACAGGCTGTCGATGACGATCCCAGACTGGCTCCAGGCGATCCAG AATTACATGAAGACCCTACAATATAATCACACAGGGACCCAGTTCTTTGAAATTAGGAAAATGAGACCG CTGAGTGGGTTAATGGAAACAGCAAAAGAAATGACCCGAGAGTCCTTGCCTATCAAATGCCTTGAAGCT GTCATCCTGGGCATCTACTTAACCAATGGGCAGCCTTCCATTGAGCGGTTCCCCATCAGCTTTAAAACC TACTTCTCAGGAAACTACTTTCACCACGTTGTGCTGGGGATTTACTGCAATGGCCGCTATGGCTCATTG GGCATGAGCCGCAGGGCTGAGCTGATGGACAAGCCATTGACTTTTCGGACTCTGAGTGACCTCATCTTT GACTTTGAGGACTCTTACAAGAAATACCTGCACACAGTCAAGAAGGTCAAGATTGGGCTGTACGTCCCC CATGAGCCTCATAGCTTCCAGCCCATTGAGTGGAAGCAGCTGGTCCTCAACGTCTCAAAGATGCTGAGG GCTGACATAAGGAAGGAGCTGGAGAAATATGCCAGGGACATGAGAATGAAGATCCTGAAACCTGCAAGT GCCCACTCTCCGACCCAAGTGAGAAGCCGGGGAAAATCCCTGTCCCCCAGAAGGAGACAGGCAAGCCCC CCGAGGAGGCTCGGCCGGCGAGAGAAGTCGCCTGCACTGCCTGAAAAGAAGGTGGCTGATCTGAGCACT CTGAATGAAGTGGGCTATCAAATCCGAATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301056
Insert Size	1068 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301056.1
RefSeq Size	4494 bp
RefSeq ORF	1068 bp
Locus ID	79805
UniProt ID	Q86V25
MW	40.4 kDa
Gene Summary	Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146869). Acts as an activator of angiogenesis: expressed in infiltrating mononuclear cells in the sprouting front to promote angiogenesis (PubMed:19204325).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) contains two alternate in-frame exons, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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