ECM1 (NM_001202858) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	URBWD
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331474 representing NM_001202858. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTGCTGCCTCTGAGGGA GGCTTCACGGCTACAGGACAGAGGCAGCTGAGGCCAGAGCACTTTCAAGAAGTTGGCTACGCAGCTCCC CCCTCCCCACCCCTATCCCGAAGCCTCCCCATGGATCACCCTGACTCCTCTCAGCATGGCCCTCCCTTT GAGGGACAGAGTGGAAAGGAGGGAAGAGGCCCTCGCCCCCACTCCCAGCCCTGGCTAGGAGAAAGGGTG GGCTGCTCACACATTCCCCCTTCTATAGTGCAGCCCCCTCCCTCTCAGGAGGCCACCCCTCTCCAACAG GAAAAGCTGCTACCTGCCCAACTCCCTGCTGAAAAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTC CCCCTCCAAAAAGAGCTGCCCTCTCTCCAGCACCCCAATGAACAGAAGGAAGGAACGCCAGCTCCATTT GGGGACCAGAGCCATCCAGAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAA GGGGGCTGGGGCCACCGGCTGGATGGCTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACCAAATC TGCCTTCCTAACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACAGTCCAGCTACTCCCACCTC ACTCGCCAGGGTGAGACCCTCAATTTCCTGGAGATTGGATATTCCCGCTGCTGCCACTGCCGCAGCCAC ACAAACCGCCTAGAGTGTGCCAAACTTGTGTGGGAGGAAGCAATGAGCCGATTCTGTGAGGCCGAGTTC TCGGTCAAGACCCGACCCCACTGGTGCTGCACGCGGCAGGGGGAGGCTCGGTTCTCCTGCTTCCAGGAG GAAGCTCCCCAGCCACACTACCAGCTCCGGGCCTGCCCCAGCCATCAGCCTGATATTTCCTCGGGTCTT GAGCTGCCTTTCCCTCCTGGGGTGCCCACATTGGACAATATCAAGAACATCTGCCACCTGAGGCGCTTC CGCTCTGTGCCACGCAACCTGCCAGCTACTGACCCCCTACAAAGGGAGCTGCTGGCACTGATCCAGCTG GAGAGGGAGTTCCAGCGCTGCTGCCGCCAGGGGAACAATCACACCTGTACATGGAAGGCCTGGGAGGAT ACCCTTGACAAATACTGTGACCGGGAGTATGCTGTGAAGACCCACCACCACTTGTGTTGCCGCCACCCT CCCAGCCCTACTCGGGATGAGTGCTTTGCCCGTCGGGCTCCTTACCCCAACTATGACCGGGACATCTTG ACCATTGACATCGGTCGAGTCACCCCCAACCTCATGGGCCACCTCTGTGGAAACCAAAGAGTTCTCACC AAGCATAAACATATTCCTGGGCTGATCCACAACATGACTGCCCGCTGCTGTGACCTGCCATTTCCAGAA CAGGCCTGCTGTGCAGAGGAGGAGAAATTAACCTTCATCAATGATCTGTGTGGTCCCCGACGTAACATC TGGCGAGACCCTGCCCTCTGCTGTTACCTGAGTCCTGGGGATGAACAGGTCAACTGCTTCAACATCAAT TATCTGAGGAACGTGGCTCTAGTGTCTGGAGACACTGAGAACGCCAAGGGCCAGGGGGAGCAGGGCTCA ACTGGAGGAACAAATATCAGCTCCACCTCTGAGCCCAAGGAAGAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001202858
Insert Size	1704 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001202858.1
RefSeq Size	2242 bp
RefSeq ORF	1704 bp
Locus ID	1893
UniProt ID	Q16610
Protein Families	Secreted Protein, Transmembrane
MW	63.6 kDa
Gene Summary	This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (3) uses alternate in-frame donor and acceptor splice sites at 2 adjacent exons compared to variant 1, resulting in a longer isoform (3) compared to isoform 1.

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