ECM1 (NM_001202858) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	URBWD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234451 representing NM_001202858 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTGCTGCCTCTGAGGGAG GCTTCACGGCTACAGGACAGAGGCAGCTGAGGCCAGAGCACTTTCAAGAAGTTGGCTACGCAGCTCCCCC CTCCCCACCCCTATCCCGAAGCCTCCCCATGGATCACCCTGACTCCTCTCAGCATGGCCCTCCCTTTGAG GGACAGAGTGGAAAGGAGGGAAGAGGCCCTCGCCCCCACTCCCAGCCCTGGCTAGGAGAAAGGGTGGGCT GCTCACACATTCCCCCTTCTATAGTGCAGCCCCCTCCCTCTCAGGAGGCCACCCCTCTCCAACAGGAAAA GCTGCTACCTGCCCAACTCCCTGCTGAAAAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTC CAAAAAGAGCTGCCCTCTCTCCAGCACCCCAATGAACAGAAGGAAGGAACGCCAGCTCCATTTGGGGACC AGAGCCATCCAGAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAAGGGGGCTG GGGCCACCGGCTGGATGGCTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACCAAATCTGCCTTCCT AACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACAGTCCAGCTACTCCCACCTCACTCGCCAGG GTGAGACCCTCAATTTCCTGGAGATTGGATATTCCCGCTGCTGCCACTGCCGCAGCCACACAAACCGCCT AGAGTGTGCCAAACTTGTGTGGGAGGAAGCAATGAGCCGATTCTGTGAGGCCGAGTTCTCGGTCAAGACC CGACCCCACTGGTGCTGCACGCGGCAGGGGGAGGCTCGGTTCTCCTGCTTCCAGGAGGAAGCTCCCCAGC CACACTACCAGCTCCGGGCCTGCCCCAGCCATCAGCCTGATATTTCCTCGGGTCTTGAGCTGCCTTTCCC TCCTGGGGTGCCCACATTGGACAATATCAAGAACATCTGCCACCTGAGGCGCTTCCGCTCTGTGCCACGC AACCTGCCAGCTACTGACCCCCTACAAAGGGAGCTGCTGGCACTGATCCAGCTGGAGAGGGAGTTCCAGC GCTGCTGCCGCCAGGGGAACAATCACACCTGTACATGGAAGGCCTGGGAGGATACCCTTGACAAATACTG TGACCGGGAGTATGCTGTGAAGACCCACCACCACTTGTGTTGCCGCCACCCTCCCAGCCCTACTCGGGAT GAGTGCTTTGCCCGTCGGGCTCCTTACCCCAACTATGACCGGGACATCTTGACCATTGACATCGGTCGAG TCACCCCCAACCTCATGGGCCACCTCTGTGGAAACCAAAGAGTTCTCACCAAGCATAAACATATTCCTGG GCTGATCCACAACATGACTGCCCGCTGCTGTGACCTGCCATTTCCAGAACAGGCCTGCTGTGCAGAGGAG GAGAAATTAACCTTCATCAATGATCTGTGTGGTCCCCGACGTAACATCTGGCGAGACCCTGCCCTCTGCT GTTACCTGAGTCCTGGGGATGAACAGGTCAACTGCTTCAACATCAATTATCTGAGGAACGTGGCTCTAGT GTCTGGAGACACTGAGAACGCCAAGGGCCAGGGGGAGCAGGGCTCAACTGGAGGAACAAATATCAGCTCC ACCTCTGAGCCCAAGGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234451 representing NM_001202858 Red=Cloning site Green=Tags(s) MGTTARAALVLTYLAVASAASEGGFTATGQRQLRPEHFQEVGYAAPPSPPLSRSLPMDHPDSSQHGPPFE GQSGKEGRGPRPHSQPWLGERVGCSHIPPSIVQPPPSQEATPLQQEKLLPAQLPAEKEVGPPLPQEAVPL QKELPSLQHPNEQKEGTPAPFGDQSHPEPESWNAAQHCQQDRSQGGWGHRLDGFPPGRPSPDNLNQICLP NRQHVVYGPWNLPQSSYSHLTRQGETLNFLEIGYSRCCHCRSHTNRLECAKLVWEEAMSRFCEAEFSVKT RPHWCCTRQGEARFSCFQEEAPQPHYQLRACPSHQPDISSGLELPFPPGVPTLDNIKNICHLRRFRSVPR NLPATDPLQRELLALIQLEREFQRCCRQGNNHTCTWKAWEDTLDKYCDREYAVKTHHHLCCRHPPSPTRD ECFARRAPYPNYDRDILTIDIGRVTPNLMGHLCGNQRVLTKHKHIPGLIHNMTARCCDLPFPEQACCAEE EKLTFINDLCGPRRNIWRDPALCCYLSPGDEQVNCFNINYLRNVALVSGDTENAKGQGEQGSTGGTNISS TSEPKEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001202858
ORF Size	1701 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001202858.2
RefSeq Size	2242 bp
RefSeq ORF	1704 bp
Locus ID	1893
UniProt ID	Q16610
Protein Families	Secreted Protein, Transmembrane
MW	64 kDa
Gene Summary	This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

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