ORC1 (NM_001190818) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSORC1; ORC1L; PARC1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331138 representing NM_001190818. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCACACTACCCCACAAGGCTGAAGACCAGAAAAACTTATTCATGGGTTGGCAGGCCCTTGTTGGAT CGAAAACTGCACTACCAAACCTATAGAGAAATGTGTGTGAAAACAGAAGGTTGTTCCACCGAGATTCAC ATCCAGATTGGACAGTTTGTGTTGATTGAAGGGGATGATGATGAAAACCCGTATGTTGCTAAATTGCTT GAGTTGTTCGAAGATGACTCTGATCCTCCTCCTAAGAAACGTGCTCGAGTACAGTGGTTTGTCCGATTC TGTGAAGTCCCTGCCTGTAAACGGCATTTGTTGGGCCGGAAGCCTGGTGCACAGGAAATATTCTGGTAT GATTACCCGGCCTGTGACAGCAACATTAATGCGGAGACCATCATTGGCCTTGTTCGGGTGATACCTTTA GCCCCAAAGGATGTGGTACCGACGAATCTGAAAAATGAGAAGACACTCTTTGTGAAACTATCCTGGAAT GAGAAGAAATTCAGGCCACTTTCCTCAGAACTATTTGCGGAGTTGAATAAACCACAAGAGAGTGCAGCC AAGTGCCAGAAACCCGTGAGAGCCAAGAGTAAGAGTGCAGAGAGCCCTTCTTGGACCCCAGCAGAACAT GTGGCCAAAAGGATTGAATCAAGGCACTCCGCCTCCAAATCTCGCCAAACTCCTACCCATCCTCTTACC CCAAGAGCCAGAAAGAGGCTGGAGCTTGGCAACTTAGGTAACCCTCAGATGTCCCAGCAGACTTCATGT GCCTCCTTGGATTCTCCAGGAAGAATAAAACGGAAAGTGGCCTTCTCGGAGATCACCTCACCTTCTAAG AGATCTCAGCCTGATAAACTTCAAACCTTGTCTCCAGCTCTGAAAGCCCCAGAGAAAACCAGAGAGACT GGACTCTCTTATACTGAGGATGACAAGAAGGCTTCACCTGAACATCGCATAATCCTGAGAACCCGAATT GCAGCTTCGAAAACCATAGACATTAGAGAGGAGAGAACACTTACCCCTATCAGTGGGGGACAGAGATCT TCAGTGGTGCCATCCGTGATTCTGAAACCAGAAAACATCAAAAAGAGGGATGCAAAAGAAGCAAAAGCC CAGAATGAAGCGACCTCTACTCCCCATCGTATCCGCAGAAAGAGTTCTGTCTTGACTATGAATCGGATT AGGCAGCAGCTTCGGTTTCTAGGTAATAGTAAAAGTGACCAAGAAGAGAAAGAGATTCTGCCAGCAGCA GAGATTTCAGACTCTAGCAGTGACGAAGAAGAGGCTTCCACACCGCCCCTTCCAAGGAGAGCACCCAGA ACTGTGTCCAGGAACCTGCGATCTTCCTTGAAGTCATCCTTACATACCCTCACGAAGGTGCCAAAGAAG AGTCTCAAGCCTAGAACGCCACGTTGTGCCGCTCCTCAGATCCGTAGTCGAAGCCTGGCTGCCCAGGAG CCAGCCAGTGTGCTGGAGGAAGCCCGACTGAGGCTGCATGTTTCTGCTGTACCTGAGTCTCTTCCCTGT CGGGAACAGGAATTCCAAGACATCTACAATTTTGTGGAAAGCAAACTCCTTGACCATACCGGAGGGTGC ATGTACATCTCCGGTGTCCCTGGGACAGGGAAGACTGCCACTGTTCATGAAGTGATACGCTGCCTGCAG CAGGCAGCCCAAGCCAATGATGTTCCTCCCTTTCAATACATTGAGGTCAATGGCATGAAGCTGACGGAG CCCCACCAAGTCTATGTGCAAATCTTGCAGAAGCTAACAGGCCAAAAAGCAACAGCCAACCATGCGGCA GAACTGCTGGCAAAGCAATTCTGCACCCGAGGGTCACCTCAGGAAACCACCGTCCTGCTTGTGGATGAG CTCGACCTTCTGTGGACTCACAAACAAGACATAATGTACAATCTCTTTGACTGGCCCACTCATAAGGAG GCCCGGCTTGTGGTCCTGGCAATTGCCAACACAATGGACCTGCCAGAGCGAATCATGATGAACCGGGTG TCCAGCCGACTGGGTCTTACCAGGATGTGCTTCCAGCCCTATACATATAGCCAGCTGCAGCAGATCCTA AGGTCCCGGCTCAAGCATCTAAAGGCCTTTGAAGATGATGCCATCCAGCTGGTAGCCAGGAAGGTAGCA GCACTGTCTGGAGATGCACGACGGTGCCTGGACATCTGCAGGCGTGCCACAGAGATCTGTGAGTTCTCC CAGCAGAAGCCTGACTCCCCTGGCCTGGTCACCATAGCCCACTCAATGGAAGCTGTGGATGAGATGTTT TCATCATCATACATCACGGCCATCAAAAATTCCTCTGTTCTGGAACAGAGCTTCCTGAGAGCCATCCTC GCAGAGTTCCGTCGATCAGGACTGGAGGAAGCCACGTTTCAACAGATATATAGTCAACATGTGGCACTG TGCAGAATGGAGGGACTGCCGTACCCCACCATGTCAGAGACCATGGCCGTGTGTTCTCACCTGGGCTCC TGTCGCCTCCTGCTTGTGGAGCCCAGCAGGAACGATCTGCTCCTTCGGGTGCGGCTCAACGTCAGCCAG GATGATGTGCTGTATGCGCTGAAAGACGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001190818
Insert Size	2586 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001190818.1
RefSeq Size	3189 bp
RefSeq ORF	2586 bp
Locus ID	4998
UniProt ID	Q13415
Protein Families	Stem cell - Pluripotency
Protein Pathways	Cell cycle
MW	97.3 kDa
Gene Summary	The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, resulting in 3 nt fewer than variant 1. Variants 1 and 2 encode the same isoform 1.

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