CNOT1 (NM_016284) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AD-005; CDC39; HPE12; NOT1; NOT1H
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308898 representing NM_016284. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAA AATTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCAT TTATTACGCTGCCTATTTTCGCATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCAT CAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATTTTATCTCGACGCTGTCC TATGCCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTG AGTAAAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGC TCAGATCTTAGAGGTTTCGCTGCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATT GACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTC CTCTCCCATCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTCTT AAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAA AAACGGGACATTCTAATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGC TCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATA ATCGTGCAGTTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGATGGCTCGA ACTCATTCAGGATTAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGT GATGGGAAAGATAAAAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTT AAAGAACTGAATCCAAGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATT CGTGACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTATGGAAGTGTTCCCA GTAGACCTCATATATAGACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATA AATCCAGAGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCA CCAGAGGATGACAATCGAGAAATTGCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTT GCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTCCCTATCAAACACTGTCCAGACATG CTGGTATTGGCCTTACTACAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTG ATGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGA CAGTCTCCCTCAATTCGCCAACTTATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTAT GATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAGGACTTGAAGGCCTTGTCAATGCTGCTAAAT GGTACTCCATTTGCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAACTTGAT AAGTGGCTCACAGATAAAATTCGAGAGCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAG AGACGGTGTCCTTCTATTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCT CCAGAAACTTTGGCGACAATGTTGGCCTGTCTGCAAGCTTGTGCAGGGAGTGTTTCTCAGGAGCTATCA GAAACTATCCTCACCATGGTAGCCAATTGCAGTAATGTTATGAATAAGGCCAGACAACCACCACCTGGA GTTATGCCAAAAGGACGTCCTCCTAGTGCTAGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCT CTTGCTGGAATGACATCTCTTAGTATAGGTGGTTCAGCTGCCCCTCACACCCAGAGTATGCAGGGTTTT CCTCCAAATTTGGGTTCTGCATTCAGTACCCCTCAGTCACCAGCAAAAGCATTTCCACCCCTTTCAACC CCCAATCAGACCACTGCATTCAGTGGTATTGGAGGACTTTCATCACAGCTTCCAGTAGGTGGTCTTGGC ACAGGCAGCCTGACTGGTATAGGAACTGGTGCTCTTGGACTCCCTGCAGTGAATAACGACCCTTTTGTA CAGAGGAAACTGGGCACCTCTGGACTGAATCAGCCTACATTCCAGCAGAGTAAGATGAAACCTTCGGAC TTGTCTCAGGTGTGGCCAGAGGCAAACCAGCACTTTAGTAAAGAGATAGATGATGAAGCAAACAGCTAT TTCCAGCGAATATATAATCATCCACCACATCCAACCATGTCTGTTGATGAGGTATTAGAAATGCTGCAG AGATTTAAAGACTCTACTATAAAGAGGGAACGAGAAGTATTTAACTGTATGCTAAGGAACTTGTTTGAA GAATATCGTTTTTTTCCCCAGTATCCTGATAAAGAGTTACATATAACAGCCTGCCTATTTGGTGGTATA ATTGAGAAAGGACTGGTCACTTACATGGCACTAGGTCTGGCTCTACGATATGTTCTTGAAGCCTTACGC AAGCCTTTTGGATCCAAAATGTATTATTTCGGGATTGCTGCACTAGATAGATTTAAAAACAGATTGAAG GACTATCCCCAGTATTGTCAGCATTTGGCTTCTATCAGTCACTTTATGCAATTTCCACATCATTTACAG GAGTATATTGAGTATGGACAGCAGTCTAGAGATCCTCCTGTGAAAATGCAAGGCTCTATCACAACCCCT GGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCTTGCTGGTCAA GTTAGCACTATGGTAACCACCTCAACAACTACCACTGTTGCTAAAACGGTTACGGTCACCAGGCCAACT GGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATTAATACTACAAATATAGATACGTTGCTTGTGGCC ACAGATCAAACTGAGAGAATTGTGGAGCCCCCAGAAAATATCCAGGAGAAAATTGCTTTTATTTTCAAT AATCTCTCACAGTCAAATATGACACAAAAGGTTGAAGAGCTAAAGGAAACGGTGAAAGAAGAATTTATG CCTTGGGTTTCACAGTATCTGGTTATGAAGAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGTATTCA AACTTCCTTGACACGCTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAGACCTACAGAAACATT AAAGTGCTCCTGACCTCTGATAAAGCTGCAGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTGGGA CATTGGCTAGGAATGATCACATTAGCTAAAAACAAACCCATCTTACACACTGACTTGGATGTGAAATCA TTGCTGCTAGAGGCTTATGTTAAAGGACAACAAGAATTGCTCTATGTAGTGCCCTTTGTTGCCAAAGTC TTAGAATCTAGCATTCGTAGTGTGGTTTTTAGGCCACCAAACCCTTGGACAATGGCAATTATGAATGTA TTAGCTGAGCTACATCAGGAGCATGACTTAAAGTTAAACTTGAAGTTTGAAATCGAGGTTCTCTGCAAG AACCTTGCATTAGACATCAATGAGCTAAAACCTGGAAACCTCCTAAAGGATAAAGATCGCCTGAAGAAT TTAGATGAGCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCAGAAGAACTCCCTCCCATCACAACC ACAACAACTTCTACTACACCAGCTACCAACACCACTTGTACAGCCACGGTTCCACCACAGCCACAGTAC AGCTACCACGACATCAATGTCTATTCCCTTGCGGGCTTGGCACCACACATTACTCTGAATCCAACAATT CCCTTGTTTCAGGCCCATCCACAGTTGAAGCAGTGTGTGCGTCAGGCAATTGAACGGGCTGTCCAGGAG CTGGTCCATCCTGTGGTGGATCGATCAATTAAGATTGCCATGACTACTTGTGAGCAAATAGTCAGGAAG GATTTTGCCCTGGATTCGGAGGAATCTCGAATGCGAATAGCAGCTCATCACATGATGCGTAACTTGACA GCTGGAATGGCTATGATTACATGCAGGGAACCTTTGCTCATGAGCATATCTACCAACTTAAAAAACAGT TTTGCCTCAGCCCTTCGTACTGCTTCCCCACAACAAAGAGAAATGATGGATCAGGCAGCTGCTCAATTA GCTCAGGACAATTGTGAGTTGGCTTGCTGTTTTATTCAGAAGACTGCAGTAGAAAAAGCAGGCCCTGAG ATGGACAAGAGATTAGCAACTGAATTTGAGCTGAGAAAACATGCTAGGCAAGAAGGACGCAGATACTGT GATCCTGTTGTTTTAACATATCAAGCTGAACGGATGCCAGAGCAAATCAGGCTGAAAGTTGGTGGTGTG GACCCAAAGCAGTTGGCTGTTTACGAAGAGTTTGCACGCAATGTTCCTGGCTTCTTACCTACAAATGAC TTAAGTCAGCCCACGGGATTTTTAGCCCAGCCCATGAAGCAAGCTTGGGCAACAGATGATGTAGCTCAG ATTTATGATAAGTGTATTACAGAACTGGAGCAACATCTACATGCCATCCCACCAACTTTGGCCATGAAC CCTCAAGCTCAGGCTCTTCGAAGTCTCTTGGAGGTTGTAGTTTTATCTCGAAACTCTCGGGATGCCATA GCTGCTCTTGGATTGCTCCAAAAGGCTGTAGAGGGCTTACTAGATGCCACAAGTGGTGCTGATGCTGAC CTTCTGCTGCGCTACAGGGAATGCCACCTCTTGGTCCTAAAAGCTCTGCAGGATGGCCGGGCATATGGG TCTCCATGGTGCAACAAACAGATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTG GAGGCTGTGGAGCTGCTAATTCGCAATCATTTGGTTAATATGCAGCAGTATGATCTTCACCTAGCGCAG TCAATGGAGAATGGCTTAAACTACATGGCTGTGGCATTTGCTATGCAGTTAGTAAAAATCCTGCTGGTG GATGAAAGGAGTGTTGCTCATGTTACTGAGGCAGATCTGTTCCACACCATTGAAACCCTCATGAGGATT AATGCTCATTCCAGAGGCAATGCTCCAGAAGGATTGCCCCAGCTGATGGAAGTAGTGCGATCCAACTAT GAAGCAATGATTGATCGTGCTCATGGAGGCCCAAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCA GAGTATGATGACCCTCCAGGCCTGAGGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTAC CATTCAGCAGCAGCTGGCCGCGACAGTACCAAAGCTTTCTCTGCATTTGTTGGACAGATGCACCAGCAA GGAATACTGAAGACCGATGATCTCATAACAAGGTTCTTTCGTCTGTGTACTGAAATGTGTGTTGAAATC AGTTACCGTGCTCAGGCTGAGCAGCAGCACAATCCTGCTGCCAATCCCACCATGATCCGAGCCAAGTGC TATCACAACCTGGATGCCTTTGTTCGACTCATTGCACTGCTCGTGAAACACTCAGGGGAGGCCACCAAC ACTGTCACAAAGATTAATCTGCTGAACAAGGTCCTTGGTATAGTAGTGGGAGTTCTCCTTCAGGATCAT GATGTTCGTCAGAGTGAATTTCAGCAACTTCCCTACCATCGAATTTTTATCATGCTTCTCTTGGAACTC AATGCACCTGAGCATGTGTTGGAAACCATTAATTTCCAGACACTTACAGCTTTCTGCAATACATTCCAC ATCTTGAGGCCTACCAAAGCTCCTGGCTTTGTATATGCCTGGCTTGAACTGATTTCCCATCGGATATTT ATTGCAAGAATGCTGGCACATACGCCACAGCAGAAGGGGTGGCCTATGTATGCACAGCTACTGATTGAT TTATTCAAATATTTAGCGCCTTTCCTTAGAAATGTGGAACTCACCAAACCTATGCAAATCCTCTACAAG GGCACTTTAAGAGTGCTGCTGGTTCTTTTGCATGATTTCCCAGAGTTCCTTTGTGATTACCATTATGGG TTCTGTGATGTGATCCCACCTAATTGTATCCAGTTAAGAAATTTGATCCTGAGTGCCTTTCCAAGAAAC ATGAGGCTCCCCGACCCATTCACTCCTAATCTAAAGGTGGACATGTTGAGTGAAATTAACATTGCTCCC CGGATTCTCACCAATTTCACTGGAGTAATGCCACCTCAGTTCAAAAAGGATTTGGATTCCTATCTTAAA ACTCGATCACCAGTCACTTTCCTGTCTGATCTGCGCAGCAACCTACAGGTATCCAATGAACCTGGGAAT CGCTACAACCTCCAGCTCATCAATGCACTGGTGCTCTATGTCGGGACTCAGGCCATTGCGCACATCCAC AACAAGGGCAGCACACCTTCAATGAGCACCATCACTCACTCAGCACACATGGATATCTTCCAGAATTTG GCTGTGGACTTGGACACTGAGGGTCGCTATCTCTTTTTGAATGCAATTGCAAATCAGCTCCGGTACCCA AATAGCCACACTCACTACTTCAGTTGCACCATGCTGTACCTTTTTGCAGAGGCCAATACGGAAGCCATC CAAGAACAGATCACAAGAGTTCTCTTGGAACGGTTGATTGTAAATAGGCCACATCCTTGGGGTCTTCTT ATTACCTTCATTGAGCTGATTAAAAACCCAGCGTTTAAGTTCTGGAACCATGAATTTGTACACTGTGCC CCAGAAATCGAAAAGTTATTCCAGTCGGTCGCACAGTGCTGCATGGGACAGAAGCAGGCCCAGCAAGTA ATGGAAGGGACAGGTGCCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_016284
Insert Size	7131 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016284.4
RefSeq Size	8476 bp
RefSeq ORF	7131 bp
Locus ID	23019
UniProt ID	A5YKK6
Domains	Not1
Protein Pathways	RNA degradation
MW	266.9 kDa
Gene Summary	Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.