DDX11 (NM_030655) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHL1, KRG2, CHLR1, MGC9335
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_030655, the custom clone sequence may differ by one or more nucleotides ATGGCTAATGAAACACAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAGG AAGACTTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGG CACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGT GAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTC TGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTT TGTGCAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGA GAAGAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAG AAGAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCT GGAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTG GCGAGCAGGGCGCCTTCTGATGCCACCTCCAGCCGCCATCCACCAGACGCCAGCTTCCCCGCTGCCCTGA ACTTCCTCCAGCGCACCAGGCCTTCCTCTGTCCTGTCTGAGGATTTGCTCATGCAACGTGCTGTGGCCAA ACACCCTGCCCTCCTCCCTTGGCAGATGTCTTCCTCTCCTTTAAGGCCTGGCTCAGAGTGGATGAGGATG AGGATGACCTGGAGGAAGAACACATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_030655 unedited GCGGCCGCGAATTCGCACGAGGCCGTTTCTAACNAGTGGCGTTTGCCCTGATTCCCGGGG CCTGGCTTTCAGCGTAGCAATTCTGGCCGGCGAAGAAGGTGAGCGCAGTGCTGTGTGGCA GCAGAGCTCCTTAGGACGAGGAGCAGCGGGACGAGGAAGGGCAGACTGGTGAAATCGCAA ACTGGGCGTCTGTTCCGGNCGCCGGACCCCTATTTGCAAAGGTCCATGGCTAATGAAACA CAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAGGAAGAC TTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCA ACTGGCACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTT GAACAGAAGAAGCGTGAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACAT GATGAGAAAGATGAATCCCTGTGTCTGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCG AGGCCTGCTGGAGAACCGGCCTGNGTTACTCAGTTTGTGCAGAAGAAAGAAGAGAGGGAC CTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGAGAAGAACGCCTGCAG CAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTTNGAGCAGAAGAAGAA GAAGAGAGATCTCCTCCGCCTCACAGGNAGTGCTANAACAGCCCNGAGCTGAGCGCTGAC ACTGGGNATTGGGGAGAAAGGACTGGTCTCGCGATACAGAGTGATAGAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_030655 unedited TTTAGGTTCTATAGCTGTGCAGGATGCAAACATCTGGGCATCAGTGACTTTTCTCCTGCC CCCGTTTGTCCCTGATGGTGGCAGAAGATCCCAGCTGACCACAGCCCCTTTCTCTGGGGT CAGAGGGAGAAGACAGGCGCAGTCAGCATGGGCAGCTGTTGCAGGTGGGAGGGATATTTT CCCACAGGAACAAAGGTCTCCGTGATGACACGGGGTCTCTATAGTCATGTTGAGAGCCTA ACGGACCTTGGCATAATTGCTGGTGTTGGTGTAGAAGGTGCCTTGCAGTTTGCTCAAGTG GTTGAGAGGGAGGGAGGTGCCATAGACTTGGACGAACTGGCTCGAGTTTTAGCTCCATAT TCCGCCCCGTCCGTGGTCTATGATAGGGAGCAGTGCCTCCTACTGTAGGTCTGACTCACG ACCGATGAGGGGCATGGTGACATGTCCCTGTTCTGGTACCTCTGTGGCCCTGGATCATTC TATTTTGTCACCATATACCGATCTGTCCATGGCACTTTCACCCTTGTCTATAAAGGCTAA ATGTAGATGGTCCCGCCTCCTTTTCCCCTCTTTTTTTCGTGGTCACGACCGCCACTTCAT CGCGGAATTCCATTTCTGCCTATTTCTCCTGCGGAGACATCAATGATTTCTGTTTTTCTT TTTGCCCTGTGTACTAACCTTTCTTTCCCTCTTGGGCTCCTGTTTGTATCCTCCATAGAT AGTCTTTCCCTCCGCCACTTAATGATTTCCATTTCTTTCTCCCGGTACCTATATCTTCGC CCTGGTCCTTGTCATCACCCGGTTCCCATTTCTTTCTTGGTCCCCCATTTGTCCCGTGTT CTCATCCATCTCCCCCGAATACTCTCTCGTTTTCTTGTCAATGCCGGCCCGGTTATGGTT GGTTTGTCTCTTCCCAATAGCCTTTTTCCTTCAATTCTCCAACCCCTCCTGCTATTTATC TGGGTGGCGCTTCACCATCAATATGCTCCCCCCTCTTTTTCCGTGTTAGATCCCCCCCCT TCGCTTTCCCCCGTATTCTT
Restriction Sites	NotI-NotI
ACCN	NM_030655
Insert Size	4100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_030655.2, NP_085913.1
RefSeq Size	4203 bp
RefSeq ORF	4203 bp
Locus ID	1663
Domains	DEXDc2
Protein Families	Stem cell - Pluripotency
Gene Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) contains exon 6, exon 21, and intron 27, as compared to variants 1 and 2. The presence of the exon 6 shifts the open reading frame and introduces an upstream stop codon within exon 7. As a result, this variant has a longer nucleotide sequence, but encodes a shorter isoform than variants 1 and 2.

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