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Separase (ESPL1) (NM_012291) Human Untagged Clone
CAT＃: SC125552
ESPL1 (untagged)-Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)
CNY 14,000.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ESP1; SEPA |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125552 sequence for NM_012291 edited (data generated by NextGen Sequencing)
ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAA GAGTTGCTGCCCGACTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGC CGATCTGATGCTGAGAGGAGACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAG CTGACTGCTAAGCTAGCTTGCCCTAGGCATCTGGGGAGCCTGCTGGAGCTGGCAGAGCTG GCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTCCCCTCTACCTGGAACGAATT CTCTTTGTCTTACTGCGGAATGCTGCTGCACAAGGAAGCCCAGAGGCCACACTCCGCCTT GCTCAGCCCCTCCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCTCCCCAGGACTAT GAGGCCGTGGCTCGGGGCAGCTTTTCTCTGCTTTGGAAGGGGGCAGAAGCCCTGTTGGAA CGGCGAGCTGCATTTGCAGCTCGGCTGAAGGCCTTGAGCTTCCTAGTACTCTTGGAGGAT GAAAGTACCCCTTGTGAGGTTCCTCACTTTGCTTCTCCAACAGCCTGTCGAGCGGTAGCT GCCCATCAGCTATTTGATGCCAGTGGCCATGGTCTAAATGAAGCAGATGCTGATTTCCTA GATGACCTGCTCTCCAGGCACGTGATCAGAGCCTTGGTGGGTGAGAGAGGGAGCTCTTCT GGGCTTCTTTCTCCCCAGAGGGCCCTCTGCCTCTTGGAGCTCACCTTGGAACACTGCCGT CGCTTTTGCTGGAGCCGCCACCATGACAAAGCCATCAGCGCAGTGGAGAAGGCTCACAGT TACCTAAGGAACACCAATCTAGCCCCTAGCCTTCAGCTATGTCAGCTGGGGGTTAAGCTG CTGCAGGTTGGGGAGGAAGGACCTCAGGCAGTGGCCAAGCTTCTGATCAAGGCATCAGCT GTCCTGAGCAAGAGTATGGAGGCACCATCACCCCCACTTCGGGCATTGTATGAGAGCTGC CAGTTCTTCCTTTCAGGCCTGGAACGAGGCACCAAGAGGCGCTATAGACTTGATGCCATT CTGAGCCTCTTTGCTTTTCTTGGAGGGTACTGCTCTCTTCTGCAGCAGCTGCGGGATGAT GGTGTGTATGGGGGCTCCTCCAAGCAACAGCAGTCTTTTCTTCAGATGTACTTTCAGGGA CTTCACCTCTACACTGTGGTGGTTTATGACTTTGCCCAAGGCTGTCAGATAGTTGATTTG GCTGACCTGACCCAACTAGTGGACAGTTGTAAATCTACCGTTGTCTGGATGCTGGAGGCC TTAGAGGGCCTGTCGGGCCAAGAGCTGACGGACCACATGGGGATGACCGCTTCTTACACC AGTAATTTGGCCTACAGCTTCTATAGTCACAAGCTCTATGCCGAGGCCTGTGCCATCTCT GAGCCGCTCTGTCAGCACCTGGGTTTGGTGAAGCCAGGCACTTATCCCGAGGTGCCTCCT GAGAAGTTGCACAGGTGCTTCCGGCTACAAGTAGAGAGTTTGAAGAAACTGGGTAAACAG GCCCAGGGCTGCAAGATGGTGATTTTGTGGCTGGCAGCCCTGCAACCCTGTAGCCCTGAA CACATGGCTGAGCCAGTCACTTTCTGGGTTCGGGTCAAGATGGATGCGGCCAGGGCTGGA GACAAGGAGCTACAGCTAAAGACTCTGCGAGACAGCCTCAGTGGCTGGGACCCGGAGACC CTGGCCCTCCTGCTGAGGGAGGAGCTGCAGGCCTACAAGGCGGTGCGGGCCGACACTGGA CAGGAACGCTTCAACATCATCTGTGACCTCCTGGAGCTGAGACCCGAGGAGACACCAGCC GGGGCCTGGGCACGAGCCACCCACCTGGTAGAACTGGCTCAGGTGCTCTGCTACCACGAC TTTACGCAGCAGACCAACTGCTCTGCTCTGGATGCTATCCGGGAAGCCCTGCAGCTTCTG GACTCTGTGAGGCCTGAGGCCCAGGCCAGAGATCAGCTTCTGGACGATAAAGCACAGGCC TTGCTGTGGCTTTACATCTGTACTCTGGAAGCCAAAATGCAGGAAGGTATCGAGCGGGAT CGGAGAGCCCAGGCCCCTGGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAA GATAAACTCCAGGAAGATCGTTTCCTATACAGTAACATTGCCTTCAACCTGGCTGCAGAT GCTGCTCAGTCCAAATGCCTGGACCAAGCCCTGGCCCTGTGGAAGGAGCTGCTTACAAAG GGGCAGGCCCCAGCTGTACGGTGTCTCCAGCAGACAGCAGCCTCACTGCAGATCCTAGCA GCCCTCTACCAGCTGGTGGCAAAGCCCATGCAGGCTCTGGAGGTCCTCCTGCTGCTACGG ATTGTCTCTGAGAGACTGAAGGACCACTCGAAGGCAGCTGGCTCCTCCTGCCACATCACC CAGCTCCTCCTGACCCTCGGCTGTCCCAGCTATGCCCAGTTACACCTGGAAGAGGCAGCA TCGAGCCTGAAGCATCTCGATCAGACTACTGACACATACCTGCTCCTTTCCCTGACCTGT GATCTGCTTCGAAGTCAACTCTACTGGACTCACCAGAAGGTGACCAAGGGTGTCTCTCTG CTGCTGTCTGTGCTTCGGGATCCTGCCCTCCAGAAGTCCTCCAAGGCTTGGTACTTGCTG CGTGTCCAGGTCCTGCAGCTGGTGGCAGCTTACCTTAGCCTCCCGTCAAACAACCTCTCA CACTCCCTGTGGGAGCAGCTCTGTGCCCAAGGCTGGCAGACACCTGAGATAGCTCTCATA GACTCCCATAAGCTCCTCCGAAGCATCATCCTCCTGCTGATGGGCAGTGACATTCTCTCA ACTCAGAAAGCAGCTGTGGAGACATCGTTTTTGGACTATGGTGAAAATCTGGTACAAAAA TGGCAGGTTCTTTCAGAGGTGCTGAGCTGCTCAGAGAAGCTGGTCTGCCACCTGGGCCGC CTGGGTAGTGTGAGTGAAGCCAAGGCCTTTTGCTTGGAGGCCCTAAAACTTACAACAAAG CTGCAGATACCACGCCAGTGTGCCCTGTTCCTGGTGCTGAAGGGCGAGCTGGAGCTGGCC CGCAATGACATTGATCTCTGTCAGTCGGACCTGCAGCAGGTTCTGTTCTTGCTTGAGTCT TGCACAGAGTTTGGTGGGGTGACTCAGCACCTGGACTCTGTGAAGAAGGTCCACCTGCAG AAGGGGAAGCAGCAGGCCCAGGTCCCCTGTCCTCCACAGCTCCCAGAGGAGGAGCTCTTC CTAAGAGGCCCTGCTCTAGAGCTGGTGGCCACTGTGGCCAAGGAGCCTGGCCCCATAGCA CCTTCTACAAACTCCTCCCCAGTCTTGAAAACCAAGCCCCAGCCCATACCCAACTTCCTG TCCCATTCACCCACCTGTGACTGCTCGCTCTGCGCCAGCCCTGTCCTCACAGCAGTCTGT CTGCGCTGGGTATTGGTCACGGCAGGGGTGAGGCTGGCCATGGGCCACCAAGCCCAGGGT CTGGATCTGCTGCAGGTCGTGCTGAAGGGCTGTCCTGAAGCCGCTGAGCGCCTCACCCAA GCTCTCCAAGCTTCCCTGAATCATAAAACACCCCCCTCCTTGGTTCCAAGCCTCTTGGAT GAGATCTTGGCTCAAGCATACACACTGTTGGCACTGGAGGGCCTGAACCAGCCATCAAAC GAGAGCCTGCAGAAGGTTCTACAGTCAGGGCTGAAGTTTGTAGCAGCACGGATACCCCAC CTAGAGCCCTGGCGAGCCAGCCTGCTCTTGATTTGGGCCCTCACAAAACTAGGTGGCCTC AGCTGCTGTACTACCCAACTTTTTGCAAGCTCCTGGGGCTGGCAGCCACCATTAATAAAA AGTGTCCCTGGCTCAGAGCCCTCTAAGACTCAGGGCCAAAAACGTTCTGGACGAGGGCGC CAAAAGTTAGCCTCTGCTCCCCTGCGCCTCAATAATACCTCTCAGAAAGGTCTGGAAGGT AGAGGACTGCCCTGCACACCTAAACCCCCAGACCGGATCAGGCAAGCTGGCCCTCATGTC CCCTTCACGGTGTTTGAGGAAGTCTGCCCTACAGAGAGCAAGCCTGAAGTACCCCAGGCC CCCAGGGTACAACAGAGAGTCCAGACGCGCCTCAAGGTGAACTTCAGTGATGACAGTGAC TTGGAAGACCCTGTCTCAGCTGAGGCCTGGCTGGCAGAGGAGCCTAAGAGACGGGGCACT GCTTCCCGGGGCCGGGGGCGAGCAAGGAAGGGCCTGAGCCTAATGACGGATGCCGTGGTT GCCCCAGGTAGTGCCCCTGGGAACCCTGGCCTGAATGGCAGGAGCCGGAGGGCCAAGAAG GTGGCATCAAGACATTGTGAGGAGCGGCGTCCCCAGAGGGCCAGTGACCAGGCCAGGCCT GGCCCTGAGATCATGAGGACCATCCCTGAGGAAGAACTGACTGACAACTGGAGAAAAATG AGCTTTGAGATCCTCAGGGGCTCTGACGGGGAAGACTCAGCCTCAGGTGGAAAGACTCCA GCTCCGGGCCCTGAGGCAGCTTCTGGAGAATGGGAGCTGCTGAGGCTGGATTCCAGCAAG AAGAAGCTGCCCAGCCCATGCCCAGACAAGGAGAGTGACAAGGACCTTGGTCCTCGGCTC CGGCTCCCCTCAGCCCCCGTAGCCACTGGTCTTTCTACCCTGGACTCCATCTGTGACTCC CTGAGTGTTGCTTTCCGGGGCATTAGTCACTGTCCTCCTAGTGGGCTCTATGCCCACCTC TGCCGCTTCCTGGCCTTGTGCCTGGGCCACCGGGATCCTTATGCCACTGCTTTCCTTGTC ACCGAGTCTGTCTCCATCACCTGTCGCCACCAGCTGCTCACCCACCTCCACAGACAGCTC AGCAAGGCCCAGAAGCACCGAGGATCACTTGAAATAGCAGACCAGCTGCAGGGGCTGAGC CTTCAGGAGATGCCTGGAGATGTCCCCCTGGCCCGCATCCAGCGCCTCTTTTCCTTCAGG GCTTTGGAATCTGGCCACTTCCCCCAGCCTGAAAAGGAGAGTTTCCAGGAGCGCCTGGCT CTGATCCCCAGTGGGGTGACTGTGTGTGTGTTGGCCCTGGCCACCCTCCAGCCCGGAACC GTGGGCAACACCCTCCTGCTGACCCGGCTGGAAAAGGACAGTCCCCCAGTCAGTGTGCAG ATTCCCACTGGCCAGAACAAGCTTCATCTGCGTTCAGTCCTGAATGAGTTTGATGCCATC CAGAAGGCACAGAAAGAGAACAGCAGCTGTACTGACAAGCGAGAATGGTGGACAGGGCGG CTGGCACTGGACCACAGGATGGAGGTTCTCATCGCTTCCCTAGAGAAGTCTGTGCTGGGC TGCTGGAAGGGGCTGCTGCTGCCGTCCAGTGAGGAGCCCGGCCCTGCCCAGGAGGCCTCC CGCCTACAGGAGCTGCTACAGGACTGTGGCTGGAAATATCCTGACCGCACTCTGCTGAAA ATCATGCTCAGTGGTGCCGGTGCCCTCACCCCTCAGGACATTCAGGCCCTGGCCTACGGG CTGTGCCCAACCCAGCCAGAGCGAGCCCAGGAGCTCCTGAATGAGGCAGTAGGACGTCTA CAGGGCCTGACAGTACCAAGCAATAGCCACCTTGTCTTGGTCCTAGACAAGGACTTGCAG AAGCTGCCGTGGGAAAGCATGCCCAGCCTCCAAGCACTGCCTGTCACCCGGCTGCCCTCC TTCCGCTTCCTACTCAGCTACTCCATCATCAAAGAGTATGGGGCCTCGCCAGTGCTGAGT CAAGGGGTGGATCCACGAAGTACCTTCTATGTCCTGAACCCTCACAATAACCTGTCAAGC ACAGAGGAGCAATTTCGAGCCAATTTCAGCAGTGAAGCTGGCTGGAGAGGAGTGGTTGGG GAGGTGCCAAGACCTGAACAGGTGCAGGAAGCCCTGACAAAGCATGATTTGTATATCTAT GCAGGGCATGGGGCTGGTGCCCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAGCTGT CGGGCAGTGGCCCTGCTGTTTGGCTGTAGCAGTGCGGCCCTGGCTGTGCATGGAAACCTG GAGGGGGCTGGCATCGTGCTCAAGTACATCATGGCTGGTTGCCCCTTGTTTCTGGGTAAT CTCTGGGATGTGACTGACCGCGACATTGACCGCTACACGGAAGCTCTGCTGCAAGGCTGG CTTGGAGCAGGCCCAGGGGCCCCCCTTCTCTACTATGTAAACCAGGCCCGCCAAGCTCCC CGACTCAAGTATCTTATTGGGGCTGCACCTATAGCCTATGGCTTGCCTGTCTCTCTGCGG TAA Clone variation with respect to NM_012291.4 74 c=>a;1842 c=>a;4304 a=>t;4551 g=>a;6110 g=>a >OriGene 5' read for NM_012291 unedited
NNCCGTCTTTCCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC GGCCGCGAATTCGGCACGAGGGGAGTACTGGTCAGGCGGTTAAGTCCTGTACCTAGGAAA GAGGGCGAGCTCTGGGGCGGTAAGGCCGGAAGGGACTCGTGAGCGTGGCTCTCCGGTGTC ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAA GAGTTGCTGCCCGACTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGC CGATCTGATGCTGAGAGGAGACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAG CTGACTGCTAAGCTAGCTTGCCCTANGCATCTGGNGAGCCTGCTGGAGCTGGCAGAGCTG GCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTCCCCTCTACCTGGAACGAATT CTCTTTGTCTTACTGGCGAATGCTGCTGCACAGGAAGCCCCAGAGCACACNTCGNCTTGG CTCAGCCCTNCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCNTCCCAGGACTATGA GGCCGTGGGCTCGGGCAGCTTTTTCTCTGCTTTTGAAGGGNGGCAGAAGCCCTGGTGGAA ACGCAAGCTGCATTTGCAGCTNCGCTGAAGGCCTTGAGCTTCTAGTACTCTTGGAGATGA AAATACCCCTTGGGAGGTTCCTTACTTTGCTTTCTCACAGCTTGTCAAGCGGTAGCTGCC CATCAGCTATTTGATGCCAGTGGCCATGGTCTAAATGAAGCANATGCTGATTTNCTAGAT GN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012291 |
| Insert Size | 7000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012291.3, NP_036423.3 |
| RefSeq Size | 6552 bp |
| RefSeq ORF | 6363 bp |
| Locus ID | 9700 |
| UniProt ID | Q14674 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Cell cycle, Oocyte meiosis |
| Gene Summary | Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010] |
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