MID1 (NM_033291) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, RNF59, ZNFXY, TRIM18
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_033291, the custom clone sequence may differ by one or more nucleotides ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGC CCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTC TGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTA GACGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGC CCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGT CCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC CAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGA ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACG TCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAAC AGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCG TTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATT CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAG AATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGA CACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCA AGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAA CCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCC AAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCA AGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGA TAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_033291 unedited NCCTGTCGGATTTTGTATACGACTCCTATAGGCGGCNCGCGAATTCGCACGAGGCTCGAG CAGAGTGCGTGTAGCAACAGATCAAAGAAAAAGAGACGAAAACGTGCTCTTTGCTGCCCG TAGATTTCGCCGGGTTGCTTTTGTCTTGCGGGCTCCTGTCGGGTTCGGTGTTTCCGCTCT GAAGACTGCGACGCGGGCTCCGATGCAGCTCGCTCCCTGCCGGATGGGTCATGGGATTCT AAACATGAGGCAGATAGCTGATCAGCTTCCTTGGGTTTTGCTGATGACACAAGAGAGCTT TGCCTGAAGATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTT GAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATC CTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCC ACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTC ACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCC AGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTC CTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGT GAAGTATCCTACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACA GGCCATCGTCTGATTGAGCCAATTCCGGACTCTCACATNNCGNNGGCTGATGTGCTTGGA GCATGANNGATGAGAAGTGGAAATATGTACTGTGTGACCGATGACCAGTTAATCTGTGCC CTGTGTAAACTGGNNTGGCGNCACCGCGATCATCAGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_033291 unedited ACTATGAACCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTGGTCTTA CAACTCTATTGTAAACTATACTAGACTATAGAGGGACTTCTACATCTTTCAAGATGTGTT TAATAAAGGTCTGTTTATAATAACTTTTGAGGCATGAATCTAGCAAATAGTACTTTATAC AATGTCCCTTGTCATTACCAACTCATAAATATTAAGTGTTTTTCAGTGACTTATGTTTGG ATGTGGTAGTGCTGATCAGGGCCATGTGCTGATGTCCTGGAGAGCAAAATCAATCCAAAG TGGTGCTGCTATTTGTGACAGAACATGTTTATTTACTCAGCCCCGGAGACAAAAGGAAAA TTGATATGGGGGAGCGGGAAATAGGAGAACTATTAAATGTAGTGAAGAAATTTCACAGGT CTAAAGGAACTATTAAAAGGAAGGATAAAGTAGATTCTATACTATAAAACAGAATCCTAC CTCTGATAAAAGACAAATCAGCCTGAATTTTTGAATAATCAATAGGATTCAAAATGACTA TTTTCAATTGCAATCTCATTCTTAATGTTCTTCCAGAACCCTTAACCCTGAGAGAAGCAG GGCAAAATGTGGCCAAAGGATTCAGAACCTTTCTTTCCAACATTATCCCATCAGCTATGA GAAGGTAAAGATTTAGCCACGGCAAGGTCCGAGGTCCAAAATCAACTCTTTTCATTCTCT GTCTTGCTCTCCATTTTAGTTGATCTGATACAACTTTTTGACATCACATTAAAACACTTC AATTATACCCNAGTAAGAAGACTGTTTTCTNCCCTACTAAGTTCAACACATCCTGTTATG CCCACTCGAGTGAGAAGACTACCCTGGGTCATCATGGGTATCAGGTAGAGATGAGTACAT CCAACTCCAAATTTTGTTTTTTGTAGCCCCGATGTGATAAATAAAGTGTAATA
Restriction Sites	NotI-NotI
ACCN	NM_033291
Insert Size	3600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_033291.1, NP_150633.1
RefSeq Size	3352 bp
RefSeq ORF	1659 bp
Locus ID	4281
Domains	zf-B_box, RING, BBC, FN3
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016] Transcript Variant: Variant 2 (beta) uses an alternate splice site near the end of the coding region, introducing a frameshift and an earlier stop codon as compared to variant 1. The beta isoform is thus 115 aa shorter than isoform alpha.

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