FOXK1 (AL390150) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL390150, the custom clone sequence may differ by one or more nucleotides GAAGGCAGCATGAGGTCAGCACACATAATTCAATTCAAAGTCATCAGACTTCATTTTTTCCCTAAAAAGT TGCTGAAAACAGAGGAAGAAGGAAGAATGCAAGGAACAAGAAAGAAAAATTGCCAGAGCTTTAAATGTCA AAGAATAATAAACACACAGGAGCAGATCATTCCGGCAGCCAGCCTTGGCTTCGTTTTCTTTTCTCCTGTC TTCCGCATCTCTGCTGGTGCTCCACTTTGTCACATACAGTTTTTGTTCCCAGATCTTAGAGTTTTATCTT TACAGATGTTCCTGTTTATTTCAAAGAAGTTATGTCACTTTTCTCTTCCCCCCCCAAGATGGTACCCACC TTATTGTAGAAGGAATGTTATCTTCGAAGCCCTTCTCGCCTCTTAGACTCAGGTGTGCCTCTATTGTAAG AGGCAAAAGTCCACGTTCTCGTGGCCTCCTTCTCTTGCTTTCCACACGCTGCTCAGCTCACTGTGCTTCT TCTGGGTTAGCCTTCTTGCTATTTCTTAAAAACCTCCTGTTTCTTACATTAATGGCAATCACCTTGCATC AACCTAATGGATTCTGTCTCAGGAGTCTTTGCACCTGCTCTTCCCACTAACTGGGATGCATTTCTGAGGA CTCACATGATCTCCTAACGGTCTCTCGCACCCGCACAGGTTATTTGAGAATCTGCTCAGATGTCACCTTG TCAGAAAGGCCTTCTTTGACCAACATCTATAAAATATCCCTTAATCCAACTCCTTTCCTCTCCTTTTATT CTTTCTTTTTTTCCTCTGAAGATGGGGCCTTCCTATGTTTCTCTGGCTGCTCTTGAGCTCCTGGTCTCCA GCAATCCTCCCACCTTGGCCTCCCATTGTACTGTGATTGCAGGCATGAGCCACCACACACAGCCCCTTTT ATTTCTTTTGATAGCGCTACTCATTACCTGGCATGACAGCACATATTAATTTGTGTACGTCTATACTTTT TATAATGAAGGTGTCACAAAATCCTGAACTGCTAGTGCAGGGGTCTATGCATTAGGGGCTCCAGAGAGAA CCCTTCTGCCATTGTGACACGCCCACTGAAGTCCCAGAGTCTATATAGAGTAGGTGCCCAATAAATATAA CTGAATGAATGAAGGTTTCTCACTTATTTCATTCATTTCTTCGCACTTGGATTCCACTTCGTTATAGAGA CTTCTGTTAAGTACCTGTAATCAAAGGACTTTCTTTTTGGTTTGAACTATGTGATTTGTTGAAAGGCTGA GGAGGGAATGAGCTTACTAAACCGTACTCCCTGTCAAAACATTTATTGGCTTTACCACACACTTAGTGAG ACCCTATTGATGCTGCATTTTGAGGGCACTAAAAACTAATAAGCCAGAAGAAGTGGCCGGCATATGTGGT CTAATAGATTCAAGGTTGAAGAATCCGCAGCGACGGTAATTTGCACAAGTCAATAAGTACTTCCTAGATT GTCTATATTTTTTAAGATACAGAGTTTTTATACCTAGTTGAGAAACAACAAAAATATGTACTTAGTGTTC TATCGTCCATGCATGCTTTATTTCCAATGAAACTCAGAGGAAATAGGTATTTCCAACAGTGTTTGCCATA GTTACACCCTTTTCTAGGGCTCTGCTCATTTTATTCCATTTCACACTCATCTTTTTCTGTTGAGAACCTA TAGAAATAATACAAGTAAGTAACATTTATTGTGCACTTTCTATGTGCCCGGCATTGTGCCAAGGCCTTTT CATGTATTTCCTCACTTAATTCCTCTACTCAATTAGGCAGGTAAAATAATATAGTTCTCATTTTAAAGAT GAGAAAACCGAGGCTTAGAAAGGCTACACAGTCAGGATATAAAGCCAGGTCTGTCCAATTTCCAAGCCTC CACTTGTAACTGCCTGGTAGACCCTCTCCTCGACTTCCTTTTCCTTTTTTTTTTTTTTTTTTTTTTGAGA TGGAGTCTCACTCTTGTGGCCCAGGCTGGAGTGCAATGGCATGATCTCAGCCCACTGCAGCCTCCACCTC CCAAGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTATAGGTGCCCACCACCATGGCT GGCTAATTTTTTTATTTTTAGTAGAGACAGGTTTCACCATGTTGGCCAGGCTAGTCTCCAGCTCCTGACC TCAGGTGATCCACCCACCTCAGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACCATGCTCAGCCGA CCCTCTCTTCTACTTCCTGAGGCCCATACAATCAAAATCCTCTGGCACCTGGAACCAGGTAGAAATGATA GGTAGAGGTGTAGAAGTCTTACAGTTACCCCAAACCCCCACAGAGGCATGTCCCTAATAATATATTTGTA AACCGACAAAAGAAAACTAGCTTTCAGAATAACACAGAAAATGAATTATTGGCTGCTACAAAAATTTAGG AATCTGGAGATCTTGGGGGCATAGATGAGGTTAACCAAGCAGCACCTGGGTCCCAGCCCAGGCTACAGGC CCCAAAGGAAGGACATGAGGACAGAACAAGGAGGAATGAGGTCCAGGAAAGAGCTGCTCATCAGATCCCA GGATTTTAAAGTATGCTGAGTGCTTTCTTCCTATTACCCTGTAGTTCCAGCTTTGATCTGAAGGGAAGAA GTTGGTTCCCTTCTAAACTAGATTTGTTTTATGACCTAAGGCATATTCATTTGGGCAACATGAGTATGAC ATTTTTCTCCCAACCATGTTAAGTCTAATTAATTTATCAAGCACTTGCCAGTTTGCCCATTCTTGCCCAT AACTACCCTCAAATCTTCAACTTTTAAGGTGAATTTGGTTTTGAAGTTACTGTCATTTCTTATGGCCTAC TGGAAATTTTCTGGTGTGTGTTTTTTTTTTTTAATGTTTGCATTTTCTAAGACTACTGTGCTTTTGTATG TATTCATTTAAATTGTCAAAAAGGATGTTGTCAAGATATGCTGAATGAGCCCCTTCCCCCAGAAATGTTG CCATATTAATGGGATATTAGCCCTTTACTGACTTGGAGGTTGCATCTTCTCAATCCGGTAATTAGCTGAA GTGCTACATTTTAAAAAATAATTTAAGCTCTTTTTTCATTATTTTTTTTTCAATTTTTCAGAAGGCAAGT TCAGACACATAGGTTTATACAAGATGAAAATATTAGATAAAAAGTGATACATCTGAATTATGTATTTTAA ACATTTTTACTTAAATACCACACATGTCCACATAGCATTATTTATACACATTTTAGTAAAACTAGCTGAG GGTGCTTGGGGGTAGGGTATTCAGTTAGTAACCTGTATCTAAGTTGATACATATCTTCCCTAACCTGTTT ACTTGATTGAATTGATTAGGGACTATTAAAAAATAAAATTATCTTTGAGAAGCAAAGAGTAAACACATAT ATAACATGGTTCTAGTTCTTGTACTGGACTCAATTCAGTCTGTAATTTTTACTGAGTCCCTACAAAGGCC TTATGCTATGATGGGTGCTACCGAAGATACATCCGTAGTTTTTGTTTTTGGTTTTTGGTTTATTTTTTTG AGACAGAGTCTCACTCTGCTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCGCTGCAACCTCCACC TCCCAGGTTCAGGCGATTCTCCTGCCTCAGCCTCCCAAGTAACTGAGATTATAGGCACTTGCCACCACGC CTGGCTAATTTTTGTATTTTTAGTGAAGACAGGATTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTC ACAGGTGATCCACCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCTACCACGCCTGGCCGA TATATCTGTTATTAATGTAGGTTTTGAAGCTTGCCCTCGAGGATTTAAAAGACAGCATTGCAATTTGCAT GTAACCTCTGAGTCAACTACTGAACTATTAAGTTGCGAATGGAAGACTTATAACCACACATGTGAATCTG CCGATTTTTTTAAAGACCTCAGTGTTGAAAAGATCATATAGAACAATAGCTAGTACTTATTAAGTGTCTA TTATGTGTCAGCTTCCTGGCATCTGTTAATTTATTTTACCTTTGTAATATTCAGGCGAGGTATGCGTTAT CCCCACTTCACAGATGAGGAAATAGGTTCCGAAAAGAAACTTACTGAAGGACACACAGAGGGTCGAGTCA GAGATCCGCTCCAAGCCCTATCTTACTCTAGTGGCCACCTTCTTTTTACTTCACATCCCATCATAAAATA TAAAACTAAACGAAGTGTTGATGGTTGTTTAATGCGAGCAATGCAGGCTGCCAAGTAGAACAATTAAGAC TCTGAAATAGCTATTTTGCTTTGACTACTTGGCTTATTTAACAATCTACAGTTACTAGCTAGATGGATAA TTGTACAGCCTCTCAATTACTTTAGGTTATGTGGACATAAGCCTGGCGCCGTTTGATTCTGACTCTAATG ATATTTAATTTCATATTCCTAATAGCTCCTTGTCTAGTGAGTATCCTAGAGGATAGAGAAAGGTACACTT TGAGGATAACCTCAGTTGCATGTACTCAAGCGGTCTTCAGGTAGCATGCATGCCCTCCCTTTAAAGAAAC TTAGTTCAGGAAAGTTAGGCTGACAAATAAATATACTAGGACATCATGGTTTCATTACAGAATGACTCTC TCTTTTAAGGGGATTCACCACCATTTTTGTCTCCTTAGCAAGACCCACTAGCATATTTAAAATATTATTT TAATATATGAATATAAATTATACCTCTTTTTAGAGATATGTCTGTCAAGTGAAGCACATTCATACCTTGA CTGGACTAAATGTTTGTCCTTATACAGAGCACAAAATGTTTCTTTCTCATTCATTCTTTCAATTTTAAAA GCCAACAGGGACCAGGGCAGTAAAGAAGAGAAACGGGCATGTGAATGTGGTCAATGGAGTCTATTCTATT GACGAATGTTTCACATTTTCTCAAACTTTTCGAGGAAATATAAAAATATGTTGCCCCAACTTCCTTTCCA ATTTCCAAAGGAACATTTTGGCTGGGAATTAGGAAAATTTCGTTCTATGTATAACATCTACGAAGATGTT TAAACTGTAATTGCATGCCATTTCAAGGAAATGTTCTTTCAGTACAGTGAAGACTGACCTACTCGGGAAT ATTAAAATGTACAGGGGTTCACATTTTGGTGATAATATTTTATCATTTTGAAAACTTATTCTTAATACAC CCCCACATTACATTTTAGTAAAATGGTTTCACTTTCTTTAACACCCATTAATAGCTATTAGAAGTAAAAA AAAAAAAAAAAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL390150 unedited NTTTTCCCCGCCCGTTGCCGCATATGGGCGTAGGCGTGTACGGTGGGAAGGTCTATATAA GCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGC GAATTCGGCACGAGGGTGAATTTGGTTTTGAAGTTACTGTCATTTCTTATGGCCTACTGG AAATTTTCTGGTGTGTGTTTTTTTTTTTTTAATGTTTGCATTTTCTAAGACTACTGTGCT TTTGTATGTATTCATTTAAATTGTCAAAAAGGATGTTGTCAAGATATGCTGAATGAGCCC CTTCCCCCAGAAATGTTGCCATATTAATGGGATATTAGCCCTTTACTGACTTGGAGGTTG CATCTTCTCAATCCGGTAATTAGCTGAAGTGCTACATTTTAAAAAATAATTTAAGCTCTT TTTTCATTATTTTTTTTTCAATTTTTCAGAAGGCAAGTTCAGACACATAGGTTTATACAA GATGAAAATATTAGATAAAAAGTGATACATCTGAATTATGTATTTTAAACATTTTTACTT AAATACCACACATGTCCACATAGCATTATTTATACACATTTTAGTAAAACTAGCTGAGGG TGCTTGGGGGTAGGGTATTCAGTTAGTAACCTGTATCTAAGTTGATACATATCTTCCCTA ACCTGTTTACTTGATTGAATTGATTAGGGACTATTAAAAAATAAAATTATCTTTGAGAAG CAAAGAGTAAACACATATATAACATGGTTCTAGTTCTTGTACTGGACTCATTCAGTCTGT AATTTTTACTGAGTCCCTACAAAGCCTTATGCTATGATGGGTGCTACCGAAGATACATCC GTAGTTTTTGTTTTTGGTTTTTAGTTTATTTTTTTGAGACAGAGTCTCACTCTGCTGCCC CAGCTGGANTGCAGTGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL390150 unedited TTAGAACCGCGCCGCAATCTAGATCGAGTTTTTTTTTTTTTTTTTTACTTCTAATAGCTA TTAATGGGTGTTAAAGAAAGTGAAACCATTTTACTAAAATGTAATGTGGGGGTGTATTAA GAATAAGTTTTCAAAATGATAAAATATTATCACCAAAATGTGAACCCCTGTACATTTTAA TATTCCCGAGTAGGTCAGTCTTCACTGTACTGAAAGAACATTTCCTTGAAATGGCATGCA ATTACAGTTTAAACATCTTCGTAGATGTTATACATAGAACGAAATTTTCCTAATTCCCAG CCAAAATGTTCCTTTGGAAATTGGAAAGGAAGTTGGGGCAACATATTTTTATATTTCCTC GAAAAGTTTGAGAAAATGTGAAACATTCGTCAATAGAATAGACTCCATTGACCACATTCA CATGCCCGTTTCTCTTCTTTACTGCCCTGGTCCCTGTTGGCTTTTAAAATTGAAAGAATG AATGAGAAAGAAACATTTTGTGCTCTGTATAAGGACAAACATTTAGTCCAGTCAAGGTAT GAATGTGCTTCACTTGACAGACATATCTCTAANAAGAGGTATAATTTATATTCATATATT AAAATAATATTTTAAATATGCTAGTGGGTCTTGCTAANGAGACAAAAATGGTGGTGAATC CCCTTAAAAGAGAGAGTCATTCTGTAATGAAACCATGATGTCCTAGTATATTTATTTGTC AGCCCTAACTTTCCTGAACTAAGTTTTCTTAAAAGGGAGGGCATGCATGCTAACCTGAGA CCGCTTGAGTACATGCAACTTGAGTTATTCTCCAAGTGTACCTTTTCTCTATTCTTCTAG AACTCCCTTANACAAGGACCTTTTAGGAATTTAAAATATATATCATTAGGGTCCGAATCA AACGGCCCCAGGCTAATGTCCCATAACCCTAATTAATTGAAAGGCTGTCAATTTT
Restriction Sites	NotI-NotI
ACCN	AL390150
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL390150.1
RefSeq Size	5332 bp
RefSeq ORF	5332 bp
Locus ID	221937
Gene Summary	Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:17670796). Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Involved in mTORC1-mediated metabolic reprogramming: in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism (By similarity). Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle (By similarity). Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population (By similarity). Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation (By similarity). Involved in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity). Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity). Represses myogenic differentiation by inhibiting MEFC activity (By similarity). Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus (PubMed:25805136). Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression (PubMed:25852164).[UniProtKB/Swiss-Prot Function]

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