Setdb1 (NM_001271175) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217580 representing NM_001271175 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCCTCCCTGGGTGCATGAGTTTGGCTGCAGCGCCAGCTGCGGCGGACTCTGCAGAGATTGCTG AGCTGCAGCAGGCAGTGGTTGAAGAGCTGGGTATCTCTATGGAGGAACTTCGTCAGTTCATCGATGAAGA ACTGGAAAAGATGGACTGTATACAGCAGCGCAAGAAGCAGCTGGCAGAGCTGGAGACGTGGGTGCTGCAG AAGGAGTCTGAGGTGGCTTATGTTGATCGGCTGTTTGATGATGCATCTAGGGAAGTGACTAATTGTGAGT CTTTGGTGAAGGATTTCTACTCTAAGCTGGGATTACAGTATCATGACAGTAGCTCTGACGATGAAGCTTC CCAGCCTACAGAGATAATCGAGATTCCTGATGAAGATGATGATGTCCTCAGTATTGATTCAGGTGATGCT GGGAGCAGAACGCCAAAAGATCAGAAGCTTCGTGAAGCTATGGCTGCCTTAAGAAAATCAGCTCAAGATG TCCAGAAGTTCATGGATGCTGTGAATAAGAAAAGCAGTTCTCAAGATCTACATAAAGGAACCTTGGGTCA GGTGTCTGGAGAACTGAGCAAAGATGGGGACCTGATAGTCAGCATGCGGATTCTGGGCAAGAAGAGGACT AAGACGTGGCACAAAGGCACCCTCATTGCCATCCAGACTGTCGGGCTAGGAAAGAAATACAAAGTGAAAT TTGACAACAAAGGAAAGAGTCTGCTGTCTGGGAACCATATTGCCTATGATTACCACCCTCCTGCTGACAA GCTGTTTGTGGGCAGTCGAGTGGTGGCCAAATACAAAGATGGAAATCAGGTCTGGCTTTATGCTGGCATT GTAGCTGAGACCCCTAACGTCAAGAACAAGCTCAGATTTTTAATTTTTTTTGATGATGGCTATGCTTCCT ATGTCACTCAGTCCGAGCTGTATCCCATTTGCCGACCATTGAAAAAGACTTGGGAGGACATAGAAGACAG CTCCTGCCGAGACTTCATAGAAGAATATATCACTGCCTATCCAAACCGCCCAATGGTACTTCTCAAGAGT GGCCAGCTTATTAAGACTGAGTGGGAAGGCACATGGTGGAAGTCTCGAGTTGAAGAGGTTGATGGCAGCC TAGTCAGGATCCTCTTTCTGGATGACAAAAGATGTGAGTGGATCTATCGAGGCTCTACACGCCTGGAACC TATGTTCAGTATGAAGACATCCTCAGCCTCTGCAATGGAGAAGAAGCAAGGGGGGCAACTCAGAACCCGT CCTAATATGGGTGCTGTGAGGAGCAAAGGTCCTGTTGTTCAGTACACTCAGGATCTAACGAGTACTGGAA TCCAGTTTAAGCCCATGGAGCCCCTGCAGCCTATAGTCCCACCTGCCCCGCTTCCCATGCTTCCTCTTTC CCCCCAGGCAGGCGACAGTGAAAGCTTAGAAAGCCAGCTTGCTCAATCACGGAAGCAAGTAGCCAAAAAG AGCACATCATTCCGACCAGGATCTGTGAGTTCTGGCCATTCCTCCCCTACTTCGCCCACACTCAGTGAAA ATGTACCCCCTGGGAAAATTGGAATGAACCAGACATACAGATCACCTTCAGCCTCTGTAACATCTACCCC AGCACCTGCAGCCCCTTCTGGGCCTCCAGCCCCTCCAGGGCCTCTTGCTCCTCCTGGACCACCGGCCCCT CCAGCTTTCCACGGCATGTTAGAGCGGGCACCAGCTGAGCCCTCCTATCGTGCCCCTATGGAGAAGCTTT TCTATTTACCTCATGTCTGCAGTTACACTTGTTTGTCCCGGATTAGACCCATGAGGAATGAACAATATCG GGGCAAAAACCCTCTACTGGTCCCACTATTGTATGACTTCCGGAGGATGACAGCACGTCGCAGAGTTAAC CGCAAGATGGGCTTCCATGTAATCTATAAGACACCCTGTGGTCTCTGCCTTCGGACGATGCAGGAAATAG AGCGCTACCTTTTTGAGACTGGCTGTGACTTCCTGTACCTGGAGATGTTCTGTTTGGATCCATATGTCCT TGTTGACAGAAAGTTCCAGCCCTTTAAACCTTTTTACTATATTTTGGACATCACCTATGGCAAAGAAGAT GTTCCCCTGTCCTGTGTTAATGAGATTGACACAACTCCCCCACCCCAGGTGGCCTACAGCAAGGAACGCA TTCCTGGCAAGGGTGTTTTCATTAACACAGGCCCTGAATTTCTGGTTGGCTGTGACTGCAAGGATGGGTG TCGGGATAAATCCAAGTGTGCCTGCCATCAGCTCACTGTCCAGGCCACAGCCTGTACCCCAGGGGGTCAG ATCAACCCTAGCTCCGGCTACCAGCACAAAAGACTAGAAGAGTGTCTGCCCACAGGGGTTTATGAGTGTA ACAAGCGCTGCAAATGTGACCCAAACATGTGCACAAATCGGTTGGTGCAGCATGGACTGCAGGTTCGACT ACAGCTGTTTAAGACACAGAACAAGGGCTGGGGCATCCGCTGCTTGGATGACATTGCTAAAGGCTCTTTT GTCTGCATTTATGCAGGCAAAATCCTGACAGATGACTTTGCAGACAAAGAAGGCCTCGAGATGGGTGATG AGTACTTTGCAAATCTGGACCACATTGAAAGTGTGGAGAACTTCAAGGAAGGATATGAGAGTGACGTCCC CAGCTCCTCTGACAGCAGTGGGGTGGATATGAAGGACCAGGAGGATGGCAACAGTGGCTCAGAGGACCCT GAGGAGTCCAACGACGACAGCTCCGATGACAACTTCTGTAAGGACGAGGACTTCAGCACCAGTTCAGTGT GGCGTAGCTATGCAACCCGGAGGCAGACTCGGGGTCAGAAGGAGAGTGAACTGTCTGAGGTGACTTCAAA GGACTCCCGCGCCCCAGACCGTGGGCCTCCCCACGTCCCTATCACTCCCTCAGGATCTGTAGGGGGTTGC AATCCACCTTCCTCCGAAGAGACACCCAAGAACAAGGTGGCCTCATGGTTGAGCTGCAATAGTGTCAGTG AAGGTGGATTTGCTGACTCTGACAGCCGTTCCTCCTTTAAGACTAGTGAAGGTGGAGATGGCCGTGCTGG GGGAGGCCGGGGAGAGGCTGAAAGAGCCTCTACCTCAGGATTGAGCTTCAAGGATGAAGGAGACAGTAAG CAATCTAAAAAAGAGGACCCTGAGGACCGAAACAAGATGTCAGTAGTTACTGAAGGCTCTCAGAATCATG GACACAATCCTCCCATGAAGTCTGAAGGGCTTCGCCGACCAGCTAGTAAAATTTCTATGCTCCAGAGCCA GCGAGTTGTGACTTCTACTCAGTCAAACCCTGATGATATCCTAACACTGTCCAGCAGCACAGAAAGTGAG GGGGAAAGTGGAACCAGCCGAAAGCCCACTACTGGTCAGACTTCAGCCACAGCAGTTGACAGTGATGACA TCCAGACCATCTCTTCTGGCTCTGATGGCGATGACTTTGAGGACAAGAAGAACTTGTCGGGACCAACAAA GCGTCAGGTGGCAGTAAAATCAACCCGAGGCTTTGCTCTTAAATCAACCCATGGTATTGCCATTAAATCA ACCAACATGGCTTCCGTGGACAAGGGGGAGAGTGCACCAGTTCGTAAGAACACACGCCAGTTCTATGATG GTGAAGAGTCTTGCTACATCATTGATGCCAAACTTGAAGGCAACCTAGGCCGCTACCTCAATCACAGTTG CAGCCCCAACCTGTTTGTCCAGAACGTTTTTGTGGATACCCATGATCTTCGCTTCCCTTGGGTGGCCTTT TTTGCCAGCAAGAGAATCCGGGCTGGAACAGAACTCACTTGGGATTACAACTATGAAGTGGGCAGTGTGG AAGGCAAGGAACTGCTGTGCTGCTGTGGAGCCATTGAATGCAGAGGGCGGCTTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217580 representing NM_001271175 Red=Cloning site Green=Tags(s) MSSLPGCMSLAAAPAAADSAEIAELQQAVVEELGISMEELRQFIDEELEKMDCIQQRKKQLAELETWVLQ KESEVAYVDRLFDDASREVTNCESLVKDFYSKLGLQYHDSSSDDEASQPTEIIEIPDEDDDVLSIDSGDA GSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDLHKGTLGQVSGELSKDGDLIVSMRILGKKRT KTWHKGTLIAIQTVGLGKKYKVKFDNKGKSLLSGNHIAYDYHPPADKLFVGSRVVAKYKDGNQVWLYAGI VAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDSSCRDFIEEYITAYPNRPMVLLKS GQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASAMEKKQGGQLRTR PNMGAVRSKGPVVQYTQDLTSTGIQFKPMEPLQPIVPPAPLPMLPLSPQAGDSESLESQLAQSRKQVAKK STSFRPGSVSSGHSSPTSPTLSENVPPGKIGMNQTYRSPSASVTSTPAPAAPSGPPAPPGPLAPPGPPAP PAFHGMLERAPAEPSYRAPMEKLFYLPHVCSYTCLSRIRPMRNEQYRGKNPLLVPLLYDFRRMTARRRVN RKMGFHVIYKTPCGLCLRTMQEIERYLFETGCDFLYLEMFCLDPYVLVDRKFQPFKPFYYILDITYGKED VPLSCVNEIDTTPPPQVAYSKERIPGKGVFINTGPEFLVGCDCKDGCRDKSKCACHQLTVQATACTPGGQ INPSSGYQHKRLEECLPTGVYECNKRCKCDPNMCTNRLVQHGLQVRLQLFKTQNKGWGIRCLDDIAKGSF VCIYAGKILTDDFADKEGLEMGDEYFANLDHIESVENFKEGYESDVPSSSDSSGVDMKDQEDGNSGSEDP EESNDDSSDDNFCKDEDFSTSSVWRSYATRRQTRGQKESELSEVTSKDSRAPDRGPPHVPITPSGSVGGC NPPSSEETPKNKVASWLSCNSVSEGGFADSDSRSSFKTSEGGDGRAGGGRGEAERASTSGLSFKDEGDSK QSKKEDPEDRNKMSVVTEGSQNHGHNPPMKSEGLRRPASKISMLQSQRVVTSTQSNPDDILTLSSSTESE GESGTSRKPTTGQTSATAVDSDDIQTISSGSDGDDFEDKKNLSGPTKRQVAVKSTRGFALKSTHGIAIKS TNMASVDKGESAPVRKNTRQFYDGEESCYIIDAKLEGNLGRYLNHSCSPNLFVQNVFVDTHDLRFPWVAF FASKRIRAGTELTWDYNYEVGSVEGKELLCCCGAIECRGRLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271175
ORF Size	3906 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271175.1, NP_001258104.1
RefSeq Size	4538 bp
RefSeq ORF	3909 bp
Locus ID	689883
MW	144 kDa

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