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Plcd1 (NM_017035) Rat Tagged ORF Clone
CAT＃: RR209950
- TrueORF®
Plcd1 (Myc-DDK-tagged ORF) - Rat phospholipase C, delta 1 (Plcd1), (10 ug)
"NM_017035" in other vectors (3)
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CNY 5,536.00
CNY 9,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Plc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR209950 representing NM_017035.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACTCGGGTAGGGACTTCCTGACCCTGCACGGGCTCCAGGATGACCCGGACCTTCAGGCCCTTCTG AAGGGCAGCCAGCTTCTGAAGGTGAAGTCCAGCTCGTGGCGTAGGGAACGCTTCTACAAGCTACAGGAG GACTGCAAGACCATCTGGCAGGAATCTCGAAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCTCCATC GAGGACATTCAGGAGGTACGGATGGGACACCGCACAGAAGGCCTGGAGAAGTTTGCCCGAGACATCCCC GAGGATCGATGCTTCTCCATTGTCTTCAAGGACCAGCGCAACACCCTAGACCTCATTGCCCCATCACCA GCTGACGCTCAGCACTGGGTGCAGGGCCTGCGCAAGATCATCCACCACTCCGGCTCCATGGACCAGCGG CAGAAGCTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGACAACAAGATGAAC TTCAAGGAGCTGAAGGACTTCCTGAAGGAGCTCAACATCCAGGTGGATGACGGCTACGCCAGGAAGATC TTCAGGGAATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAAGATG CTGACCCAGCGCGCAGAGATCGACCGTGCTTTTGAGGAGGCCGCAGGATCCGCGGAGACCCTGTCGGTG GAGAGGCTAGTGACGTTTCTGCAGCACCAGCAGCGGGAGGAGGAGGCAGGGCCAGCCTTGGCCCTCTCT CTCATTGAGCGCTACGAGCCCAGTGAGACTGCCAAGGCCCAGCGGCAGATGACCAAGGATGGCTTCCTC ATGTACTTGCTGTCGGCTGACGGTAACGCCTTCAGCCTGGCACACCGCCGTGTCTACCAGGACATGGAC CAGCCACTGAGTCACTACTTAGTGTCTTCTTCCCACAACACCTACCTGCTGGAAGACCAGCTCACAGGG CCCAGCAGCACGGAGGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGCTTGGAGCTCGACTGCTGG GATGGTCCCAACCAGGAACCCATCATCTACCACGGCTACACTTTTACCTCTAAGATACTTTTCTGTGAC GTGCTCAGGGCCATCAGGGACTATGCCTTCAAGGCATCCCCCTACCCAGTCATCCTGTCCCTGGAGAAC CACTGTAGCCTGGAGCAACAACGAGTCATGGCCCGTCACCTGAGGGCTATCCTGGGGCCCATACTGTTG GACCAGCCACTGGATGGGGTTACCACGAGCCTGCCTTCACCTGAGCAACTGAAGGGCAAGATCCTGTTG AAAGGGAAGAAGCTGGGAGGGCTGCTGCCTGCTGGAGGGGAGAATGGGTCCGAAGCCACTGACGTGTCT GACGAGGTAGAGGCTGCTGAAATGGAGGACGAGGCTGTGCGCAGCCAAGTGCAACACAAGCCCAAGGAG GATAAACTAAAGCTGGTGCCGGAGCTCTCCGACATGATCATTTACTGCAAGAGCGTCCACTTTGGTGGC TTCTCCAGTCCTGGCACCTCTGGGCAGGCATTCTATGAGATGGCTTCCTTCTCTGAGAGCCGTGCCCTC CGGTTGCTGCAAGAATCAGGAAATGGTTTTGTCCGTCATAACGTGAGCTGTCTGAGCAGGATCTACCCG GCTGGGTGGAGAACAGATTCCTCCAACTACAGTCCTGTGGAGATGTGGAACGGGGGCTGCCAGATTGTT GCTCTGAACTTCCAAACCCCTGGGCCAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGC TGTGGGTATGTGCTGAAACCCGCCTTTCTGAGAGACCCCAACACCACCTTCAACTCACGTGCCCTGACT CAGGGGCCCTGGTGGCGTCCAGAGAGGCTCCGTGTCCGGATCATCTCTGGACAGCAGCTGCCAAAGGTC AATAAGAATAAGAATTCTATCGTGGACCCCAAGGTGATCGTGGAGATCCATGGCGTGGGCCGGGACACC GGTAGCCGTCAGACAGCAGTTATTACCAATAATGGTTTCAACCCACGGTGGGACATGGAGTTTGAGTTT GAGGTAACAGTGCCTGACCTTGCCCTCGTACGCTTCATGGTAGAGGATTATGATTCCTCTTCTAAGAAC GACTTTATTGGCCAGAGTACTATCCCCTGGAACAGCCTCAAGCAGGGGTACCGCCATGTCCACCTCTTG TCTAAGAATGGAGACCAGCATCCATCAGCCACACTTTTTGTGAAGATCTCCATCCAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RR209950
Blue=ORF Red=Cloning site Green=Tag(s) MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSI EDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQR QKLQHWIHSCLRKADKNKDNKMNFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKM LTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFL MYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCW DGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMARHLRAILGPILL DQPLDGVTTSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHKPKE DKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYP AGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALT QGPWWRPERLRVRIISGQQLPKVNKNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEF EVTVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017035 |
| ORF Size | 2268 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017035.1, NP_058731.1 |
| RefSeq Size | 2791 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 24655 |
| UniProt ID | P10688 |
| MW | 86 kDa |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN209950 | Plcd1 (untagged ORF) - Rat phospholipase C, delta 1 (Plcd1), (10 ug) |
CNY 9,790.00 |
|
| RR209950L3 | Lenti ORF clone of Plcd1 (Myc-DDK-tagged ORF) - Rat phospholipase C, delta 1 (Plcd1), (10 ug) |
CNY 11,590.00 |
|
| RR209950L4 | Lenti ORF clone of Plcd1 (mGFP-tagged ORF) - Rat phospholipase C, delta 1 (Plcd1), (10 ug) |
CNY 12,640.00 |
