Mybpc3 (NM_001106490) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207982 representing NM_001106490 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGCCAGGGAAGAGACCAGTGTCAGCCTTCACCAAGAAGCCCAGGTCAGTGGAGGTGACCGCAG GCAGTGCTGCTGTGTTCGAGGCTGAGACGGAGCGGTCAGGCCTGAAGGTGCAGTGGCAGCGGGATGGCAG TGATATCGCTGCCAATGACAAGTATGGTTTGGCAGCAGAGGGCAAACGACACACACTGACAGTGCGGGAT GTGGGCCCTGACGATCAGGGTTCCTATGCGGTCATCGCAGGCTCCTCAAAGGTCAAGTTTGACCTTAAGG TCACAGAGCCAGCCCCTCCAGAGAAGGCAGAGTCTGCAGTTGCTCCAACCTCTATGGAAGCTCCAGAAAC TCCCAAAGAAGTTCCTGCTCTAGCCACTCAGTTGGAAGGAAATGTCTCAAGTCCTGAAGGGTCAGTCTCG GTAACCCAGGATGGCTCAGTCGCAGGGAGTCAGGGAGCCCCTGACGACCCTATTGGCCTCTTTCTGATGC GGCCACAGGATGGCGAGGTGACCGTGGGCGGCAGCATCGTCTTCTCAGCCCGAGTGGCGGGAGCCAGCCT CCTGAAACCGCCTGTAGTCAAGTGGTTTAAGGGCAAGTGGGTGGACCTGAGCAGCAAGGTGGGCCAGCAC CTGCAGCTGCACGACAGCTACGACAGAGCCAGCAAGGTCTACTTGTTTGAGTTGCACATCACAGATGCGC AGGCCACTTCTGCTGGGGGCTACCGCTGTGAGGTGTCTACCAAGGACAAATTTGACAGCTGCAACTTCAA CCTCACTGTACATGAGGCCATTGGTTCTGGAGACCTGGACCTCAGATCAGCTTTCCGACGCACGAGCCTG GCTGGAACGGGTCGGAGAACCAGTGACAGCCATGAAGATGCTGGGACTCTGGACTTTAGCTCCCTGCTGA AGAAGAGCAGTTTCCGGAGGGACTCAAAGCTAGAAGCACCCGCTGAAGAAGATGTGTGGGAGATCCTGAG ACAGGCGCCACCGTCAGAATATGAGCGCATCGCCTTCCAGCACGGAGTCACAGACCTGCGAGGCATGCTG AAGAGGCTCAAGGGCATGAAGCACGATGAAAAGAAGAGCACAGCCTTTCAGAAGAAGCTGGAGCCTGCCT ACCAGGTAAACAAGGGCCACAAGATCCGGCTTACTGTGGAACTGGCTGATCCAGACGCCGAAGTCAAGTG GCTTAAGAATGGACAGGAGATCCAGATGAGTGGCAGGTACATCTTTGAGTCCATCGGTGCCAAGCGTACC CTGACCATCAGCCAGTGCTCACTGGCTGACGATGCAGCCTACCAGTGTGTGGTGGGGGGTGAGAAATGCA GCACAGAGCTCTTTGTCAAAGAACCCCCGGTGCTGATCACTCGGTCCCTGGAAGACCAGCTAGTGATGGT GGGTCAGCGGGTGGAGTTTGAGTGTGAGGTCTCAGAAGAAGGGGCCCAAGTCAAATGGCTGAAGGATGGG GTTGAGCTGACTCGGGAGGAGACCTTCAAATACCGGTTCAAGAAAGATGGACGGAAGCACCACTTGATCA TCAACGAAGCGACCCTGGAGGACGCAGGACACTATGCAGTTCGCACAAGTGGGGGCCAAGCACTGGCTGA GCTCATTGTGCAAGAGAAGAAGCTGGAGGTCTACCAAAGCATCGCGGACCTGGCGGTGGGAGCGAAGGAC CAGGCTGTGTTTAAGTGTGAGGTTTCAGATGAGAACGTGCGTGGCGTGTGGCTGAAGAACGGGAAGGAAC TGGTGCCGGACAACCGCATAAAGGTGTCCCATATAGGCCGGGTCCACAAACTGACCATTGACGATGTCAC ACCTGCCGATGAGGCTGACTACAGTTTTGTGCCTGAAGGGTTTGCCTGCAACCTGTCTGCCAAGCTCCAC TTCATGGAGGTCAAGATTGACTTTGTGCCTAGGCAGGAACCTCCCAAGATCCACTTGGACTGTCCTGGCA GCACACCAGACACCATTGTGGTTGTTGCTGGGAACAAGTTACGCCTGGATGTCCCTATTTCTGGGGACCC TGCTCCCACTGTAATCTGGCAAAAGACTATAACACAGGGGAAGAAGGCCTCAGCTGGGCCACCCCCTGGT GCCCCAGAAGATGCAGGTGCTGATGAGGAGTGGGTGTTTGATAAGAAGCTGTTGTGTGAGACGGAGGGCC GTGTCCGTGTGGAGACCACCAAAGACCGAAGCGTCTTCACGGTCGAAGGGGCGGAGAAGGAAGATGAGGG TGTCTACACTGTCACAGTAAAGAACCCCGTGGGCGAGGACCAGGTCAACCTCACAGTCAAGGTCATTGAT GTGCCAGATGCTCCTGCAGCCCCTAAGATCAGCAACGTGGGCGAGGACTCCTGCATTGTGCAGTGGGAAC CACCTGCCTATGATGGTGGGCAGCCGGTCCTGGGATACATCCTGGAGCGCAAGAAGAAAAAGAGCTACAG GTGGATGAGGCTCAACTTCGATCTGCTGCGGGAGCTGAGCCACGAAGCCAGGCGCATGATTGAGGGTGTA GCCTATGAGATGAGAGTCTACGCGGTCAATGCTGTGGGAATGTCCAGGCCCAGCCCTGCCTCTCAGCCCT TCATGCCTATTGGGCCCCCTGGTGAACCAACTCACCTGACTGTGGAGGATGTGTCGGACACCACAGTCTC ACTCAAGTGGCGGCCCCCAGAGCGTGTGGGTGCAGGTGGCCTGGACGGATACAGTGTGGAGTATTGCCAG GAGGGCTGCTCCGAGTGGGTGACTGCTCTGCAGGGGTTGACAGAGCGCACTTCGCTGCTGGTGAAGGACC TACCCACTGGGGCACGGCTGCTGTTCCGAGTGCGGGCACACAATGTGGCAGGTCCTGGGGGCCCTATCAT CACCAAGGAGCCTGTAACAGTGCAGGAGATACTGCAGCGACCACGGCTCCAACTGCCCAGACACCTGCGC CAGACCATCCAGAAGAAAGTTGGGGAGCCTGTGAACCTCCTCATCCCTTTCCAGGGCAAACCCCGGCCTC AAGTGACCTGGACAAAAGAGGGGCAGCCACTGGCAGGCGAGGAGGTGAGCATCCGCAACAGCCCCACAGA CACAATCTTGTTCATCCGAGCTGCCCACCGCACTCACTCGGGCACCTACCAGGTGACAGTTCGCATTGAG AACATGGAGGACAAGGCAACGCTGGTCCTGCAGATCGTGGACAAGCCAAGTCCTCCCCTGGATATCCGGG TCGTTGAGACTTGGGGCTTCAGTGTGGCTCTGGAGTGGAAGCCACCCCAAGATGATGGCAATACAGAGAT CTGGGGTTATACTGTACAGAAAGCCGACAAGAAGACCATGGAGTGGTTCACTGTTTTGGAACATTACCGT CAGACTCACTGTGTGGTATCAGAGCTTATCATTGGCAATGGCTACTACTTCCGGGTCTTCAGCCATAACA TGGTGGGCTCCAGTGACAGAGCTGCTGCCACCAAGGAGCCCATCTTTATTCCGAGACCAGGCATCACATA TGAGCCACCCAAATACAAGGCCCTGGACTTCTCTGAAGCCCCAAGCTTCACCCAGCCCTTGACAAACCGC TCCATCATTGCAGGCTACAATGCCATCCTCTGCTGTGCTGTCCGGGGTAGTCCTAAGCCCAAGATTTCCT GGTTCAAGAATGGCTTGGACCTGGGAGAAGATGCTCGTTTTCGCATGTTCTGCAAGCAGGGAGTATTGAC CCTGGAGATCAGGAAGCCCTGTCCCTATGATGGGGGTGTCTATGTCTGCAGAGCCACCAACTTGCAGGGT GAGGCACAGTGTGAGTGCCGCCTGGAGGTGCGAGTTCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207982 representing NM_001106490 Red=Cloning site Green=Tags(s) MPEPGKRPVSAFTKKPRSVEVTAGSAAVFEAETERSGLKVQWQRDGSDIAANDKYGLAAEGKRHTLTVRD VGPDDQGSYAVIAGSSKVKFDLKVTEPAPPEKAESAVAPTSMEAPETPKEVPALATQLEGNVSSPEGSVS VTQDGSVAGSQGAPDDPIGLFLMRPQDGEVTVGGSIVFSARVAGASLLKPPVVKWFKGKWVDLSSKVGQH LQLHDSYDRASKVYLFELHITDAQATSAGGYRCEVSTKDKFDSCNFNLTVHEAIGSGDLDLRSAFRRTSL AGTGRRTSDSHEDAGTLDFSSLLKKSSFRRDSKLEAPAEEDVWEILRQAPPSEYERIAFQHGVTDLRGML KRLKGMKHDEKKSTAFQKKLEPAYQVNKGHKIRLTVELADPDAEVKWLKNGQEIQMSGRYIFESIGAKRT LTISQCSLADDAAYQCVVGGEKCSTELFVKEPPVLITRSLEDQLVMVGQRVEFECEVSEEGAQVKWLKDG VELTREETFKYRFKKDGRKHHLIINEATLEDAGHYAVRTSGGQALAELIVQEKKLEVYQSIADLAVGAKD QAVFKCEVSDENVRGVWLKNGKELVPDNRIKVSHIGRVHKLTIDDVTPADEADYSFVPEGFACNLSAKLH FMEVKIDFVPRQEPPKIHLDCPGSTPDTIVVVAGNKLRLDVPISGDPAPTVIWQKTITQGKKASAGPPPG APEDAGADEEWVFDKKLLCETEGRVRVETTKDRSVFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVID VPDAPAAPKISNVGEDSCIVQWEPPAYDGGQPVLGYILERKKKKSYRWMRLNFDLLRELSHEARRMIEGV AYEMRVYAVNAVGMSRPSPASQPFMPIGPPGEPTHLTVEDVSDTTVSLKWRPPERVGAGGLDGYSVEYCQ EGCSEWVTALQGLTERTSLLVKDLPTGARLLFRVRAHNVAGPGGPIITKEPVTVQEILQRPRLQLPRHLR QTIQKKVGEPVNLLIPFQGKPRPQVTWTKEGQPLAGEEVSIRNSPTDTILFIRAAHRTHSGTYQVTVRIE NMEDKATLVLQIVDKPSPPLDIRVVETWGFSVALEWKPPQDDGNTEIWGYTVQKADKKTMEWFTVLEHYR QTHCVVSELIIGNGYYFRVFSHNMVGSSDRAAATKEPIFIPRPGITYEPPKYKALDFSEAPSFTQPLTNR SIIAGYNAILCCAVRGSPKPKISWFKNGLDLGEDARFRMFCKQGVLTLEIRKPCPYDGGVYVCRATNLQG EAQCECRLEVRVPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106490
ORF Size	3822 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106490.1, NP_001099960.1
RefSeq Size	4165 bp
RefSeq ORF	3825 bp
Locus ID	295929
UniProt ID	P56741
MW	140.8 kDa
Gene Summary	Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.[UniProtKB/Swiss-Prot Function]

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